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Isolation and Characterization of Eukaryotic Translation Initiation, Elongation and Termination Factors

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Post-transcriptional Control of Gene Expression

Part of the book series: NATO ASI Series ((ASIH,volume 97))

Abstract

Initiation factors were first isolated from mammalian cells. Using a cell-free system containing salt-washed rat ribosomal subunits, rabbit globin mRNA, charged tRNAs, ATP, GTP and purified translation elongation factors, Schreier, Erni and Staehelin (Schreier et al., 1977) defined a number of proteins from a rabbit ribosomal salt wash fraction, which were required to promote globin synthesis in vitro. These proteins were purified to homogeneity and shown to be required for the binding of initiator Met-tRNA and mRNA to ribosomes (Trachsel et al., 1977, for a review, see Hershey, 1991). These factors and factors isolated in other laboratories together with some of their properties are listed in Table 1.

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Author information

Authors and Affiliations

  1. Institute of Biochemistry and Molecular Biology, University of Berne, Bühlstrasse 28, 3012, Berne, Switzerland

    H. Trachsel

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  1. H. Trachsel
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Editors and Affiliations

  1. Biological Laboratories, Harvard University, 16 Divinity Avenue, 02138, Cambridge, MA, USA

    Orna Resnekov

  2. Department of Microbiology and Genetics, Vienna Biocenter, Dr. Bhor-Gasse 9, A-1030, Vienna, Austria

    Alexander von Gabain

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© 1996 Springer-Verlag Berlin Heidelberg

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Trachsel, H. (1996). Isolation and Characterization of Eukaryotic Translation Initiation, Elongation and Termination Factors. In: Resnekov, O., von Gabain, A. (eds) Post-transcriptional Control of Gene Expression. NATO ASI Series, vol 97. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60929-9_14

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  • DOI: https://doi.org/10.1007/978-3-642-60929-9_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64609-6

  • Online ISBN: 978-3-642-60929-9

  • eBook Packages: Springer Book Archive

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