Abstract
In the present study we investigated the effects of norepinephrine and vasoactive intestinal peptide on the intracellular calcium concentration ([Ca2+]i) and on phosphorylation of the transcription factor CREB in isolated rat pinealocytes. Norepinephrine evoked a biphasic calcium response via activation of α1-adrenergic receptors in virtually all pinealocytes identified by the S-antigen immunoreaction. This response involved mobilization of calcium from intracellular calcium stores and calcium influx into the cell. Moreover, norepinephrine induced phosphorylation of CREB via activation of β-adrenergic receptors. In contrast, vasoactive intestinal peptide did not cause an elevation of [Ca2+]i, but induced pCREB immunoreactivity in a subpopulation of the pinealocytes, suggesting their functional heterogeneity.
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References
Chik CL, Ho AK, Klein DC (1988) Dual receptor regulation of cyclic nucleotides: α1adrenergic potentiation of vasoactive intestinal peptide stimulation of pinealocyte cAMP. Endocrinology 122: 1646–1651
De Groot RP, Sassone-Corsi P (1993) Hormonal control of gene expression: multiplicity and versatility of cyclic adenosine 3’, 5’-monophosphate-responsive nuclear regulators. Mol Endocrinol 7: 145–153
Gonzalez GA, Montminy MR (1989) Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133. Cell 59: 675–680
Kaneko T, Cheng PY, Oka H, Oda T, Yanaihara N, Yanaihara C (1980) Vasoactive intestinal polypeptide stimulates adenylate cyclase and serotonin N-acetyltransferase activities in rat pineal gland in vitro. Biochem Res 1: 84–87
Klein DC (1985) Photoneural regulation of the mammalian pineal gland. In: Evered D, Clark S (ed) Photoperiodism, melatonin and the pineal gland. Pitman, London, pp 38–56
Korf HW (1996) Innervation of the pineal gland. In: Burnstock G (ed) Handbook of the autonomic nervous system. Harwood, Chur, pp 129–180
Korf HW, Oksche A, Ekstrom P, Zigler JS, Gery I, Klein DC (1986) Pinealocyte projections into the mammalian brain revealed with S-antigen antiserum. Science 231: 735–737
Letz B, Schomerus C, Maronde E, Korf HW, Korbmacher C (1997) Stimulation of a nicotinic ACh receptor causes depolarization and activation of L-type Ca2+ channels in rat pinealocytes. J Physiol 499: 329–340
Olcese J, McArdle C, Mikkelsen J, Hannibal J (1996) PACAP and type I PACAP receptors in the pineal gland. Ann NY Acad Sci 805: 595–600
Roseboom PH, Klein DC (1995) Norepinephrine stimulation of pineal cyclic AMP response element-binding protein phosphorylation: involvement of a ?-adrenergic/cyclic AMP mechanism. Mol Pharmacol 47: 439–449
Schaad NC, Parfitt A, Russell JT, Schaffner AE, Korf HW, Klein DC (1993) Single cell Ca2+ analysis and biochemical characterization of pinealocytes immobilized with a novel attachment peptide preparation. Brain Res 614: 251–256
Schaad NC, Vanacek J, Rodriguez IR, Klein DC, Holtzclaw L, Russell JT (1995) Vasoactive intestinal peptide elevates pinealocyte intracellular calcium concentrations by enhancing influx: evidence for involvement of a cyclic GMP-dependent mechanism. Mol Pharmacol 47: 923–933
Schomerus C, Ruth P, Korf HW (1994) Photoreceptor-specific proteins in the mammalian pineal organ: immunocytochemical data and functional considerations. Acta Neurobiol Exp 54 (Suppl): 9–17
Schomerus C, Laedtke E, Korf HW (1995) Calcium responses of isolated, immuno-cytochemically identified rat pinealocytes to noradrenergic, cholinergic and vasopressinergic stimulations. Neurochem Int 27: 163–175
Schomerus C, Maronde E, Laedtke E, Korf HW (1996) Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) induce phosphorylation of the transcription factor CREB in subpopulations of rat pinealocytes: immunocytochemical and immunochemical evidence. Cell Tissue Res 286: 305–313
Sheng M, Greenberg, ME (1990) The regulation and function of c-fos and other immediate early genes in the nervous system. Neuroscience 4: 477–485
Stehle JH, Foulkes NS, Molina CA, Simonneaux V, Pevet P, Sassone-Corsi P (1993) Adrenergic signals direct rhythmic expression of transcriptional repressor CREM in the pineal gland. Nature 356: 314–320
Sugden AL, Sugden D, Klein DC (1986) Essential role of calcium influx in the adrenergic regulation of cAMP and cGMP in rat pinealocytes. J Biol Chem 261: 11608–11612
Tamotsu S, Schomerus C, Stehle JH, Roseboom P, Korf HW (1995) Norepinephrine-induced phosphorylation of the transcription factor CREB in isolated rat pinealocytes: an immunocytochemical study. Cell Tissue Res 282: 219–226
Wicht H, Korf HW, Schaad NC (1993) Morphological and immunocytochemical heterogeneity of cultured pinealocytes from one-week and two-month-old rats: planimetric and densitometric investigations. J Pineal Res 14: 128–137
Yamamoto KK, Gonzalez GA, Biggs WH, Montminy MR (1988) Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. Nature 334: 494–498
Yuwiler A (1983) Vasoactive intestinal peptide stimulation of pineal serotonin-N-acetyltransferase activity. J Neurochem 41: 146–153
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© 1997 Springer-Verlag Berlin Heidelberg
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Schomerus, C., Korf, HW. (1997). Noradrenergic and VIPergic Signal Transduction in Rat Pinealocytes. In: Korf, HW., Usadel, KH. (eds) Neuroendocrinology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60915-2_26
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DOI: https://doi.org/10.1007/978-3-642-60915-2_26
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