Novel Microscope-Based Approaches for the Investigation of Protein-Protein Interactions in Signal Transduction

  • György Vereb
  • Christoph K. Meyer
  • Thomas M. Jovin
Part of the NATO ASI Series book series (volume 102)


In addition to the biochemical characterization of interacting proteins, the spatiotemporal localisation in situ or in vivo of the transiently associating participants of a cascade mechanism can greatly enhance our understanding of the given signal transduction process. However, the mere determination of colocalization based on fluorescent tags that are compatible with the in vivo observation does not generally suffice due to the limitation in resolution imposed by optical diffraction in conventional light microscopy.


Fluorescence Resonance Energy Transfer Scanning Force Microscopy Fluorescence Resonance Energy Transfer Efficiency Conventional Light Microscopy Fluorescence Resonance Energy Transfer Signal 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • György Vereb
    • 1
    • 2
  • Christoph K. Meyer
    • 1
  • Thomas M. Jovin
    • 1
  1. 1.Department of Molecular BiologyMax Planck Institute for Biophysical ChemistryGöttingenGermany
  2. 2.Department of BiophysicsUniversity Medical School of DebrecenDebrecenHungary

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