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Characterisation of the actin-binding protein Insertin

  • Andreas Teubner
  • Helmet E. Meyer
  • Albrecht Wegner
Conference paper
Part of the NATO ASI Series book series (volume 102)

Abstract

Insertin is an actin-binding protein which copurifies with vinculin from chicken gizzard. It binds to the barbed ends of actin filaments thereby modulating the polymerisation rate. In contrast to other barbed-end proteins (“capping proteins”) insertin does not fully suppress the polymerisation, but retards it maximally to one fifth of the normal rate of polymerisation. Thus, insertin is able to permit actin polymerisation while bound to the filament ends and is therefore a good candidate for fine tuning actin polymerisation. Localisation of insertin in focal adhesion plaques (dynamic cell-substrate contacts of migrating cells) underlines this potenial role and raises the question of possible interactions with other focal adhesion proteins.

Keywords

Focal Adhesion Northern Blot Analysis Focal Adhesion Protein Chicken Gizzard cDNA Library Screening 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Davis, et al., Science, 252, 712–715, 1991.PubMedCrossRefGoogle Scholar
  2. 2.
    Weigt et al., J. Mol. Biol., 227, 593–595, 1992PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Andreas Teubner
    • 1
  • Helmet E. Meyer
    • 1
  • Albrecht Wegner
    • 1
  1. 1.Institute for Physiological ChemistryRuhr-University BochumBochumGermany

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