Abstract
Primary cultures of astroblasts were prepared from cerebral cortices of 14- to 16-day-old Wistar rat embryos (E 14–16) as described previously (Wunderlich et al. 1994). Meninges were detached and cortices, suspended in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco, Eggenstein, Germany), were triturated with fire-polished Pasteur pipettes. Debris and cell aggregates were removed by passage through 60-μm gaze. Cell suspension was centrifuged and resuspended in DMEM supplemented with 10% fetal calf serum (FCS; Gibco) before being plated into plastic culture flasks.
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© 1997 Springer-Verlag Berlin Heidelberg
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Stichel-Gunkel, C.C. (1997). Materials and Methods. In: The Role of Microenvironment in Axonal Regeneration. Advances in Anatomy Embryology and Cell Biology, vol 137. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60781-3_3
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DOI: https://doi.org/10.1007/978-3-642-60781-3_3
Publisher Name: Springer, Berlin, Heidelberg
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