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Maturation Phenomenon in Cerebral Ischemia II pp 33–40Cite as

Is Apoptosis Involved in Glutamate Neurotoxicity?

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Summary

Recent studies on glutamate neurotoxicity suggest that apoptosis plays a role in excitotoxic neuronal death. The present study was designed to examine the possible involvement of apoptosis in such death.

In the first part of the study we employed Nomarski optics combined with a digital image processor to examine the acute type of glutamate neurotoxicity, and found a rapid change in the nucleus followed by cellular swelling. These changes could be prevented by treatment with MK-801, a N-methyl-d-aspartate (NMDA) receptor antagonist. These results indicate that morphological changes mediated by overactivation of NMDA receptor does not fulfill the morphological criteria of apoptosis, but do suggest that the nuclear disintegration is involved as a leading step in acute-type glutamate excitotoxicity.

In the second part of the study, we examined the effects of a peptide inhibitor for interleukin-l β-converting enzyme (ICE) and a free radical scavenger (MCI-186) on the delayed type of glutamate neurotoxicity. Whereas the ICE inhibitor failed to show a beneficial effect, MCI-186 significantly reduced the excitotoxic damage, suggesting that apoptosis may be involved in the delayed type of neuronal death, and that free radical production may be related to this process.

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Author information

Authors and Affiliations

  1. Department of Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, 15-45 Yushima, Bunkyo-ku, Tokyo, 113, Japan

    J. Ikeda, I. Morita & S. Murota

  2. Asai Hospital, 38-1 Katoku, Togane-shi Chiba, 283, Japan

    J. Ikeda & K. Asai

  3. Photon Medical Center Hamanatsu University School of Medicine, 3600 Handa-maclin, Shizuoka, 431-31, Japan

    S. Terakawa

  4. Department of Neurosurgery, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku Tokyo, 113, Japan

    K. Hirakawa

Authors
  1. J. Ikeda
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  2. S. Terakawa
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  3. K. Asai
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  4. I. Morita
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  5. S. Murota
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  6. K. Hirakawa
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Editor information

Editors and Affiliations

  1. Department of Neurosurgery, Musashino Red Cross Hospital, 1-26-1, Kyonan-cho, 180, Musashino-shi, Tokyo, Japan

    Umeo Ito

  2. Faculty of Medicine Department of Neurosurgery, University of Tokyo, 7-3-1, Hongo, 113, Bunkyo-ku, Tokyo, Japan

    Takaaki Kirino

  3. Department of Neuropathology Medical Research Institute, Tokyo Medical and Dental University, Yushima 1-5-45, 113, Bunkyo-ku, Tokyo, Japan

    Toshihiko Kuroiwa

  4. National Institutes of Health, Bethesda, MD, 20892-4128, USA

    Igor Klatzo (Former Chief of the Laboratory of Neuropathology and Neuroanatomical Sciences) (Former Chief of the Laboratory of Neuropathology and Neuroanatomical Sciences)

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© 1997 Springer-Verlag Berlin Heidelberg

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Ikeda, J., Terakawa, S., Asai, K., Morita, I., Murota, S., Hirakawa, K. (1997). Is Apoptosis Involved in Glutamate Neurotoxicity?. In: Ito, U., Kirino, T., Kuroiwa, T., Klatzo, I. (eds) Maturation Phenomenon in Cerebral Ischemia II. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60546-8_5

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  • DOI: https://doi.org/10.1007/978-3-642-60546-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-61673-3

  • Online ISBN: 978-3-642-60546-8

  • eBook Packages: Springer Book Archive

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