Abstract
The wealth of sequence data generated in the last 20 years has revealed the existence of many multigene families (MacIntyre 1994; Ohta 1991). Genes within a family typically share short stretches of conserved primary sequence, usually as part of conserved functional domains. Genes within a family may also play similar or overlapping roles in diverse tissues and processes. In some cases, individual genes within a family are differentially expressed, with up-regulation coinciding with a requirement for the gene product in a specific biological process. Examples include the differential expression of homeobox containing genes during development (Lawrence and Morata 1994; Scott and Weiner 1984) and altered abundance of members of the cyclin gene family during various phases of the cell cycle (Pines 1995; Hunt 1991). Thus, for processes in which specific gene families are suspected to play a role, an assay that allows for the rapid detection and cloning of differentially expressed family members would be of great value. Targeted RNA fingerprinting (TRF) was developed to respond to such a need.
Keywords
- DEPC Treated Water
- Reverse Transcription Primer
- Amplification Buffer
- Specific Gene Family
- Conserve Code Region
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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© 1997 Springer-Verlag Berlin Heidelberg
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Stone, B. (1997). Targeted RNA Fingerprinting. In: Micheli, M.R., Bova, R. (eds) Fingerprinting Methods Based on Arbitrarily Primed PCR. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60441-6_38
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DOI: https://doi.org/10.1007/978-3-642-60441-6_38
Publisher Name: Springer, Berlin, Heidelberg
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