DNA Extraction from Mammals
Part of the
Springer Lab Manuals
book series (SLM)
DNA fingerprinting methods based on PCR amplification using arbitrary primers such as RAPD (Williams et al. 1990), AP-PCR (Welsh and McClelland 1990) and DAF (Caetano-Anollés et al. 1991) require minimal amounts of DNA (generally a few ng per reaction); however, it has been shown that these methods may be significantly influenced by the quality of the template. The choice of an extraction procedure yielding high molecular weight DNA (free of contaminants which can interfere with the amplification process) is, therefore, an essential prerequisite in obtaining reliable DNA fingerprints. Furthermore, it is important that the same extraction procedure is used for all DNAs to be compared.
KeywordsArbitrary Primer Full Speed Capillary Pipette Saturated Phenol Benchtop Centrifuge
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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