Advertisement

DNA Extraction from Mammals

  • E. D’Ambrosio
  • E. Pascale
Part of the Springer Lab Manuals book series (SLM)

Abstract

DNA fingerprinting methods based on PCR amplification using arbitrary primers such as RAPD (Williams et al. 1990), AP-PCR (Welsh and McClelland 1990) and DAF (Caetano-Anollés et al. 1991) require minimal amounts of DNA (generally a few ng per reaction); however, it has been shown that these methods may be significantly influenced by the quality of the template. The choice of an extraction procedure yielding high molecular weight DNA (free of contaminants which can interfere with the amplification process) is, therefore, an essential prerequisite in obtaining reliable DNA fingerprints. Furthermore, it is important that the same extraction procedure is used for all DNAs to be compared.

Keywords

Arbitrary Primer Full Speed Capillary Pipette Saturated Phenol Benchtop Centrifuge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Caetano-Anollés G, Bassam BJ, Gresshoff PM (1991) DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/Technology 9:553 – 557PubMedCrossRefGoogle Scholar
  2. Micheli MR, Bova R, Pascale E, D’Ambrosio E (1994) Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method. Nucleic Acids Res 22:1921–1922PubMedCrossRefGoogle Scholar
  3. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleate cells. Nucleic Acids Res 16:1215PubMedCrossRefGoogle Scholar
  4. Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218PubMedCrossRefGoogle Scholar
  5. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • E. D’Ambrosio
  • E. Pascale

There are no affiliations available

Personalised recommendations