Advertisement

Detection of Minimal Tumor Cell Populations by FICTION

  • J. Deerberg
  • A. Rosenwald
  • K. Weber-Matthiesen
  • B. Schlegelberger
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 38)

Abstract

Sensitive techniques for the detection of minimal tumor cell populations are gaining in importance. In hematological disorders they are required for monitoring residual disease after therapy, detection of minimal bone marrow infiltration by malignant lymphoma cells and exclusion of tumor cell contamination after leukapheresis. The applicability of the combination of immunophenotyping and interphase cytogenetics, the FICTION technique, to detection of minimal tumor cell populations is demonstrated in a case of Hodgkin’s disease.

Keywords

Minimal Residual Disease Chromosome Aberration Acute Lymphatic Leukemia Cytospin Slide Mosome Aberration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cremer T, Landegent J, Bruckner A, Scholl H, Schardin M, Devilee P, Pearson P, Van der Ploog M (1986) Detection of chromosome aberrations in the human interphase nucleus by visualization of specific target DNAs. Hum Genet 74: 346–352PubMedCrossRefGoogle Scholar
  2. 2.
    Weber-Matthiesen K, Winkemann M, Muller Hermelink A, Schlegelberger B, Grote W (1992) Simultaneous fluorescence immunophenotyping and interphase cytogenetics: a contribution to the characterization of tumor cells. J Histochem Cytochem 40: 171–175PubMedCrossRefGoogle Scholar
  3. 3.
    Weber-Matthiesen K, Deerberg J, Muller-Hermelink A, Schlegelberger B, Grote W (1993) Rapid immunophenotypic characterization of chromosomally aberrant cells by the new FICTION method. Cytogenet Cell Genet 63: 123–125CrossRefGoogle Scholar
  4. 4.
    Weber-Matthiesen K, Muller-Hermelink A, Deerberg J, Scherthan H, Schlegelberger B, Grote W (1993) Discrimination of distinct subpopulations within a tumor with double immunophenotyping and interphase cytogenetics. J Histochem Cytochem 41: 1641–1644PubMedCrossRefGoogle Scholar
  5. 5.
    Weber-Matthiesen K, Pressl S, Schlegelberger B, Grote W (1993) Combined immunophenotyping and interphase cytogenetics on cryostat sections by the new FICTION method. Leukemia 7: 646–649PubMedGoogle Scholar
  6. 6.
    Tilly H, Bastard C, Delastre T, Duval C, Bizet M, Lenormand B, Dauce J-P, Monconduit M, Piquet H (1991) Cytogenetic studies in untreated Hodgkin’s disease. Blood 77: 1298–1304PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • J. Deerberg
    • 1
  • A. Rosenwald
    • 1
  • K. Weber-Matthiesen
    • 1
  • B. Schlegelberger
    • 1
  1. 1.Department of Human GeneticsUniversity of KielGermany

Personalised recommendations