Stimulation of Growth and Proliferation of Human Cultured Keratinocytes by KGF-transfected Cells in vitro

  • J. Kopp
  • F. P. Dai
  • P. Kulmburg
  • E. Tanczos
  • C. Andree
  • X. Y. Jiao
  • Y. Chen
  • R. Horch
  • G. B. Stark
Conference paper


Keratinocyte Growth Factor (KGF), a 28 kD polypeptide induces mitosis, proliferation and migration of keratinocytes. The objective of this study was the establishment of two stably KGF-transfected, immortalized cell lines, Ha-CaT-keratinocytes and KMST-6-fibroblasts, by liposome mediated gene transfer. Transfection effectivity, gene integration and configuration of the transgenic protein was investigated by ELISA, DANN-PCR and ß-Gal-staining. Most effective KGF producing clones were tested by a colorimetrie XTT-test, demonstrating a significant acceleration of cell proliferation and mitosis of human keratinocytes in a Air Liquid Interface (ALI) test system.


Keratinocyte Growth Factor Immortalize Cell Line HaCaT Keratinocytes Peptide Growth Factor Polypyrimidine Tract Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Aaronson SA, Rubin JS, Finch PW, Wong J, Marchese C, Falco J, Taylor WG, Kraus MH (1990) Growth factor-regulated pathways in epithelial cell proliferation. [Review]. Am Rev Respir Dis 142:S7–10PubMedGoogle Scholar
  2. 2.
    Boukamp P, Petrussevska RT, Breitkreutz D, Hornung J, Markham A, Fusenig NE (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 106 (3):761ffGoogle Scholar
  3. 3.
    Boyce ST, Ham RG (1983) Calcium-regulated differentiation of normal human keratinocytes in chemically definied clonal culture and serum-free serial cultures. J Invest Dermatol, 81:33s–40sPubMedCrossRefGoogle Scholar
  4. 4.
    Brown GL, Curtsinger L, Jurkiewicz MJ, Nahai F, Schultz G (1991) Stimulation of healing of chronic wounds by epidermal growth factor. Plast Reconstr Surg 88:189 ffGoogle Scholar
  5. 5.
    Dlugosz AA, Cheng C, Denning MF, Dempsey PJ, Coffey RJ Jr, Yuspa SH (1994) Keratinocyte growth factor receptor ligands induce transforming growth factor alpha expression and activate the epidermal growth factor receptor signaling pathway in cultured epidermal keratinocytes. Cell Growth Differ 5:1283–1292PubMedGoogle Scholar
  6. 6.
    Finch PW, Lengel C, Chedid M (1995) Cloning and characterization of the promoter region of the human keratinocyte growth factor gene. J Biol Chem 270:11230–11237PubMedCrossRefGoogle Scholar
  7. 7.
    Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H (1995) Transcriptional activation by tetracyclines in mammalian cells. Science 268:1766–1769PubMedCrossRefGoogle Scholar
  8. 8.
    Kanzler MH, Gorsulowsky DC, Swanson NA (1986) Basic mechanisms in the healing of cutaneous wounds. J Dermatol Surg Oncol 12:1156PubMedGoogle Scholar
  9. 9.
    Latkowski JM, Freedberg IM, Blumenberg M (1995) Keratinocyte growth factor and keratin gene regulation. J Dermatol Sci 9:36–44PubMedCrossRefGoogle Scholar
  10. 10.
    Namba M, Nishitani K, Hyodoh F, Fukushima F, Kimoto T (1985) Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays. Int J Cancer 35:275–80PubMedCrossRefGoogle Scholar
  11. 11.
    Pessa ME, Bland KI, Copeland EM III (1987) Growth factors and determinants of wound repair. J Surg Res 42:207PubMedCrossRefGoogle Scholar
  12. 12.
    Rubin JS, Bottaro DP, Chedid M, Miki T, Ron D, Cheon G, Taylor WG, Fortney E, Sakata H, Finch PW et al (1995) Keratinocyte growth factor [Review]. Cell Biol Int 19:399–411PubMedCrossRefGoogle Scholar
  13. 13.
    Rubin JS, Osada H, Finch PW, Taylor WG, Rudikoff S, Aaronson SA (1989) Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc Natl Acad Sci USA 86:802–806PubMedCrossRefGoogle Scholar
  14. 14.
    Sotozono C, Kinoshita S, Kita M, Imanishi J (1994) Paracrine role of keratinocyte growth factor in rabbit corneal epithelial cell growth. Exp Eye Res 59:385–391PubMedCrossRefGoogle Scholar
  15. 15.
    Sporn MB, Roberts AB (1986) Peptide growth factors and inflammation, tissue repair and cancer. J Clin Invest 78:329PubMedCrossRefGoogle Scholar
  16. 16.
    Steenfos HH (1992) Growth factors and formation granulation tissue. Thesis University Göteborg Google Scholar
  17. 17.
    Witherell GW, Gil A, Wimmer E (1993) Interaction of polypyrimidine tract binding protein with the encephalomyocarditis virus mRNA internal ribosomal entry site. Biochemistry 32:268–8275CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. Kopp
  • F. P. Dai
  • P. Kulmburg
  • E. Tanczos
  • C. Andree
  • X. Y. Jiao
  • Y. Chen
  • R. Horch
  • G. B. Stark

There are no affiliations available

Personalised recommendations