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Stimulation of Growth and Proliferation of Human Cultured Keratinocytes by KGF-transfected Cells in vitro

  • J. Kopp
  • F. P. Dai
  • P. Kulmburg
  • E. Tanczos
  • C. Andree
  • X. Y. Jiao
  • Y. Chen
  • R. Horch
  • G. B. Stark
Conference paper

Abstract

Keratinocyte Growth Factor (KGF), a 28 kD polypeptide induces mitosis, proliferation and migration of keratinocytes. The objective of this study was the establishment of two stably KGF-transfected, immortalized cell lines, Ha-CaT-keratinocytes and KMST-6-fibroblasts, by liposome mediated gene transfer. Transfection effectivity, gene integration and configuration of the transgenic protein was investigated by ELISA, DANN-PCR and ß-Gal-staining. Most effective KGF producing clones were tested by a colorimetrie XTT-test, demonstrating a significant acceleration of cell proliferation and mitosis of human keratinocytes in a Air Liquid Interface (ALI) test system.

Keywords

Keratinocyte Growth Factor Immortalize Cell Line HaCaT Keratinocytes Peptide Growth Factor Polypyrimidine Tract Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • J. Kopp
  • F. P. Dai
  • P. Kulmburg
  • E. Tanczos
  • C. Andree
  • X. Y. Jiao
  • Y. Chen
  • R. Horch
  • G. B. Stark

There are no affiliations available

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