Wound Healing, Fluid and Protein Balance in Severe Burns Following Topical Application of Clotting Factors
Aim of the study was to investigate whether a combination of clotting factors with homologous full thickness skin grafts would accelerate wound healing and diminish fluid and protein losses following severe third degree burns.
To this purpose in vivo studies where performed using Wistar rats. All animals had third degree burns extending 20% of total body surface. Group A received a full thickness skin graft with topical application of clotting factors to the wound bed, group B received a skin graft without clotting factors, and group C received no transplant.
The results show that the topical application of clotting factors (Tissucol®) significantly accelerated wound healing as well as blood volume and intravascular albumin normalization following burns.
Conclusion: Wound bed sealing by use of topical application of clotting factors may become a reliable tool in burn treatment to enhance recovery following third degree burns.
KeywordsFormalin Catheter Albumin Citrate Syringe
Unable to display preview. Download preview PDF.
- 2.Bader A, Rinkes IHB, Closs EI, Ryan CM, Toner M, Cunningham JM, Tompkins RG, Yarmush ML (1992) A stable long-term hepatocyte culture system for studies of physiologic processes: Cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotechnol Progress 8:219–225.CrossRefGoogle Scholar
- Burke JF (1979) Primary burn excision and immediate grafting: a method shortening illness. J Trauma 14:389–394 d.Google Scholar
- 5.Burke JF (1979) Fluid therapy to reduce morbidity. J Trauma 11:856–886.Google Scholar
- 9.Gallico CG, O’Connor N, Compton C (1984) Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 7:448–451 10.Google Scholar