Tissue Engineering of Skeletal Muscle Using Micropatterned Biomaterials
Muscle transfer is a common procedure in plastic and reconstructive as well as other surgeries but is associated with a risk of morbidity for the donor area. Fabricating skeletal muscle tissue in vitro offers an alternative to this procedure. The key to creating tissue engineered skeletal muscle is fabricating my fibers aligned parallel to each other in culture. Micro patterned biomaterials composed of alternating adhesive and non-adhesive regions are a system which allows control of the architecture neo-muscle in two dimensions. The aim of this present study is to identify the effects of a series of un-micro patterned model biomaterials on the attachment, morphology, and proliferation of myoblasts, as well as to evaluate how the evolution of micropat-terns of these cells depends on the geometry of the micro pattern and the density of initial plating. We find that attachment, projected area, and proliferation for myoblasts are much greater on adhesive biomaterials than on non-adhesive biomaterials. Further, myoblasts on micro patterned biomaterials attach selectively to adhesive regions. Our studies suggest that two-dimensional control of the architecture of tissue culture of myoblasts can form the bases for tissue engineering skeletal muscle.
KeywordsTissue Engineer Plating Density Initial Plating Adhesive Region Skeletal Muscle Satellite Cell
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