Delivery Systems for Osteoinductive Proteins

Conference paper


Bone morphogenetic proteins (BMPs) have been investigated for three decades. The fact that demineralized bone and extracts of demineralized bone are osteoinductive was first shown by Urist [1]. The temporal sequence of bone induction was described by Reddi and co-workers [2]. Of the various growth and differentiative factors known, only the native and recombinant BMPs have shown the ability to singularly induce de novo bone formation both in vitro and in vivo. Their principle function is to induce transformation of undifferentiated mesenchymal cells into chondroblasts and/or osteoblasts.


Bone Morphogenetic Protein Fibrin Glue Collagen Matrix Glass Ceramic Collagen Sponge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Urist MR (1965) Bone formation by autoinduction. Science 150:893–899.PubMedCrossRefGoogle Scholar
  2. 2.
    Reddi AH, Wientroub S, Muthukumaran N (1987) Biologic principles of bone induction. Orthop Clin North Am 189:207–212.Google Scholar
  3. 3.
    Hollinger JO, Seyfer AE (1994) Bioactive factors and biosynthetic materials in bone grafting. Clin Plast Surg 21:415–418.PubMedGoogle Scholar
  4. 4.
    Urist MR, Iwata H, Ceccotti PC, Dorfman RL, Boyd SD, McDowell RM, Chien C (1973) Bone morphogenesis in implants of insoluble bone gelatine. Proc Natl Acad Sci USA 70:3511–3515.PubMedCrossRefGoogle Scholar
  5. 5.
    Urist MR, Lietze A, Dawson E (1984) β-tricalcium phosphate delivery system for bone morphogenetic protein. Clin Orthop 187:277–280.PubMedGoogle Scholar
  6. 6.
    Hotz G, Herr G (1994) Bone subsitute with osteoinductive biomaterials: current and future clinical applications. Int J Oral Maxillofac Surg 23:413–417.PubMedCrossRefGoogle Scholar
  7. Hotz G (1991) Knochenersatz mit osteoplastischen und osteoinduktiven Biomaterialien unter Verwendung des Fibrinklebesystems. (Immuno, Vienna, ISSN 0177–6967) Ellipse 29, 435–440.Google Scholar
  8. 8.
    Hotz G (1993) The osteoinductive potential of bone morphogenetic gelatin complexed with hydroxylapatite and fibrin glue. In: Schlag G, Redl H. (eds) Wound healing. Springer, Berlin Heidelberg New York London Paris, pp 127–135.Google Scholar
  9. 9.
    Kawamura M, Urist MR (1988) Human fibrin is a physiologic delivery system for bone morphogenetic protein. Clin Orthop 235:302–310.PubMedGoogle Scholar
  10. Hotz G, Reif D, Herr G (1996) Evaluation of absorbable glass ceramics as carriers for BMP. Cranio Maxillofac Surg 24, suppl. 1:56.Google Scholar
  11. 11.
    Cobb CM, Howell BE, Cray RC, Weatherford TW (1976) Potential of elastin and collagen as initiators of in vivo calcification. Oral Surg 41:24–31.PubMedCrossRefGoogle Scholar
  12. 12.
    Deporter DA, Shiga A, Melcher AH, Howley TP (1985) The effect of skin collagen (Zy-derm) on the healing of experimental defects on the rat calvarium. In Dixon AD, Sarrnat BG (eds) Normal and abnormal growth: basic and clinical research. Liss AR, New York, pp 353–363.Google Scholar
  13. 13.
    Werntz J, Lane JM, Piez K, Seyedin S, Burstein A (1986) Repair of segmental bone defects with collagen and marrow. ORS 32nd Annual Meeting, New Orleans, Louisiana, Feb 17–20, p 108.Google Scholar
  14. 14.
    Deatherage JR, Miller EJ (1987) Packaging and delivery of bone induction factors in a collagenous implant. Coll Rel Res 7:225–231.Google Scholar
  15. 15.
    Hotz G, Herr G, Krempien B, Schulze-Icking P (1995) Kollagen-Formkörper als Wirkstoffdepot für osteoinduktive Proteine. Z Zahnärztl Implantol 11:170–175.Google Scholar
  16. 16.
    Landsmann R, Reddi AH (1989) In vivo analysis of the half-life of the osteoinductive potential of demineralized bone matrix using diffusion chambers. Calcif Tissue Int 45:348–353.CrossRefGoogle Scholar
  17. 17.
    Hollinger JO, Leong K (1996) Poly (a-hydroxy acids): carriers for bone morphogenetic proteins. Biomaterials 17:187–194.PubMedCrossRefGoogle Scholar
  18. 18.
    Bessho K, Tagawa, T, Murata, M (1989) Purification of bone morphogenetic protein derived from bovine bone matrix. Biochem Biophys Res Commun 165:595–601.PubMedCrossRefGoogle Scholar
  19. 19.
    Takaoka K, Koezuka M, Nakahara H (1991) Telopeptide-depleted bovine skin collagen as a carrier for bone morphogenetic protein. J Orthop Res 9:902–907.PubMedCrossRefGoogle Scholar
  20. 20.
    Ripamonti U (1991) The morphogenesis of bone in replicas of porous hydroxylapatite obtained from conversion of calcium carbonate exoskeletons of coral. J Bone Joint Surg 5:692–703.Google Scholar
  21. 21.
    Katoh T, Sato K, Kawamura M, Iwata H, Miura T (1993) Osteogenesis in sintered bone combined with bovine bone morphogenetic protein. Clin Orthop 287:266–275.PubMedGoogle Scholar
  22. 22.
    Kawamura M, Iwata H, Sato K, Miura T (1987) Chondroosteogenetic response to crude bone matrix proteins bound to hydroxylapatite. Clin Orthop 217:281–292.PubMedGoogle Scholar
  23. 23.
    Yamazaki Y, Oida S, Akimoto Y, Shioda S (1988) Response of the mouse femoral muscle to an implant of a composite of bone morphogenetic protein and plaster of paris. Clin Orthop 234:240–246.PubMedGoogle Scholar
  24. 24.
    Schmitz JP, Hollinger JO (1988) A preliminary study of the osteogenic potential of a biodegradable alloplastic-osteoinductive alloimplant. Clin Orthop 237:245–255.PubMedGoogle Scholar
  25. 25.
    Lovell TP, Dawson EG, Nilsson OS, Urist MR (1989) Augmentation of spinal fusion with bone morphogenetic protein in dogs. Clin Orthop 243:266–274.PubMedGoogle Scholar
  26. 26.
    Takaoka K, Nakahara H, Yoshikawa H, Masuhara K, Tsuda T, Ono K (1988) Ectopic bone induction on and in porous hydroxyl apatite combined with collagen and bone morphogenetic protein. Clin Orthop 234:250–254.PubMedGoogle Scholar
  27. 27.
    Damien CJ, Parsons JR, Benedict JJ, Weisman DS (1990) Investigation of a hydroxyapatite and calcium sulfate composite supplemented with an osteoinductive factor. J Biomed Mater Res 24:639–654.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • G. Hotz

There are no affiliations available

Personalised recommendations