In vitro Engineered Cartilage for Reconstructive Surgery, Using Biocompatible, Resorbable Fibrin Glue/Polymer Structures
Tissue engineering, one of the most challenging fields in research, offers new revolutionary perspectives in solving problems concerning tissue replacement. Current practical approaches in cartilage engineering still face problems with three-dimensional cell distribution, or they require components for cell immobilization which raise biocompatibility problems. In this study, we shall present a model using cells crosslinked by fibrin within biocompatible resorbable polymers. Both components have been in clinical use for a long time. Immunohistochemical procedures have shown that this model provides optimal requirements for in vitro cartilage production. Cartilage-specific extracellular components like proteoglycan, chondroitin sulfate and collagen II have been immunochemically characterizized. Histomorphological methods showed a mechanically stable tissue compound for at least six weeks. We suggest that this model fulfillls all the biocompatible requirements for in vitro production of autologous, individually shaped cartilage transplants for reconstructive surgery.
KeywordsFibrin Glue Engineer Cartilage Plasma Protein Fraction Dedifferentiated Chondrocytes Cartilage Engineering
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