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Fibrin Glue as a Carrier for Cultured Human Keratinocytes versus Cultured Epidermal Skin Grafts in Athymic Mice Full-thickness Wounds

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Biological Matrices and Tissue Reconstruction

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Summary

Cultured epidermal sheet autografts (CEA) have become a standard method to treat extensive burns and have been utilized to resurface chronic ulcers. However, several problems associated with the laboratory steps in processing such grafts, their handling, the graft take rates and the clinical outcome have still not been solved. To circumvent some of these disadvantages the subsequent transplantation after preconditioning the wounds with allografts, the in vitro fabrication of composite grafts, and the evolution of new techniques to deliver cultured keratinocytes have been continuously developed. Fibrin glue, frequently used in skin grafting procedures in burn patients, may provide an appropriate carrier to deliver cultured keratinocytes onto a wound bed. We were able to show clinically that transplanting cultured human keratinocytes suspended as single cells in a fibrin glue matrix (KFGS) in combination with meshed allografts leads to stable wound closure in burn patients. In an athymic mouse model with reproducible, standardized, full-thickness wounds, this new technique was compared directly to CEA. Whereas the time for reepithelialization was similar in both groups, reconstitution of the dermo-epidermal junction zone — as shown by electron microscopy and immuno-histochemistry — was significantly enhanced by the fibrin-glue suspension technique. Therefore, it may be concluded that the new KFGS technique is not only available earlier than sheet grafts and simplifies the application, but is also able to transfer single, undifferentia ed keratinocytes which are in the state of mitosis and active proliferation.

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Authors
  1. R. Horch
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  2. H. Bannasch
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  3. C. Andree
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  4. J. Kopp
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  5. G. B. Stark
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Editors and Affiliations

  1. Abt. Plastische und Handchirurgie, Chirurgische Universitätsklinik Freiburg, Hugstetter Straße 55, D-79106, Freiburg, Germany

    G. Björn Stark , Raymund Horch  & Eszter TÁczos ,  & 

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© 1998 Springer-Verlag Berlin Heidelberg

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Horch, R., Bannasch, H., Andree, C., Kopp, J., Stark, G.B. (1998). Fibrin Glue as a Carrier for Cultured Human Keratinocytes versus Cultured Epidermal Skin Grafts in Athymic Mice Full-thickness Wounds. In: Stark, G.B., Horch, R., TÁczos, E. (eds) Biological Matrices and Tissue Reconstruction. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60309-9_10

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  • DOI: https://doi.org/10.1007/978-3-642-60309-9_10

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64347-7

  • Online ISBN: 978-3-642-60309-9

  • eBook Packages: Springer Book Archive

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