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Untargeted Approaches to the Analysis of Gene Epression in Arbuscular Mycorrhiza: Analyses of Polypeptides (in Vivo and in Vitro) and of Translated mRNA Products

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Mycorrhiza Manual

Part of the book series: Springer Lab Manual ((SLM))

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Abstract

Current gel electrophoretic methods such as native or SDS-PAGE and two-dimensional 2D-PAGE have been widely utilized as untargeted approaches to study proteins in arbuscular mycorrhizas (Dumas et al. 1989; Schellenbaum et al. 1992; Arines et al. 1993; Dumas-Gaudot et al. 1994; Simoneau et al. 1994; Samra et al. 1996). Although 1D-PAGE has been useful to demonstrate differences in polypeptide patterns between uninoculated and several plants inoculated with different AM fungi (Dumas et al. 1989; Schellenbaum et al. 1992; Arines et al. 1993), the low resolution of this technique quickly limited its application in detecting mycorrhiza-specific polypeptides. For this reason, we have adopted 2DPAGE protocols to study modifications in root polypeptide profiles with mycorrhizal development (Tahiri-Alaoui et al. 1991; Dumas-Gaudot et al. 1994; Gianinazzi-Pearson 1995; Samra et al. 1996).

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Authors and Affiliations

  1. Laboratoire de Phytoparasitologie, INRA/CNRS, C.M.S.E., I.N.R.A., BV 1540, 21034, Dijon Cedex, France

    E. Dumas-Gaudot, A. Samra, V. Gianinazzi-Pearson & S. Gianinazzi

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  1. E. Dumas-Gaudot
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  2. A. Samra
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  3. V. Gianinazzi-Pearson
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  4. S. Gianinazzi
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Editors and Affiliations

  1. School of Life Sciences, Jawaharlal Nehru University, 110067, New Delhi, India

    Ajit Varma

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© 1998 Springer-Verlag Berlin Heidelberg

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Dumas-Gaudot, E., Samra, A., Gianinazzi-Pearson, V., Gianinazzi, S. (1998). Untargeted Approaches to the Analysis of Gene Epression in Arbuscular Mycorrhiza: Analyses of Polypeptides (in Vivo and in Vitro) and of Translated mRNA Products. In: Varma, A. (eds) Mycorrhiza Manual. Springer Lab Manual. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60268-9_20

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  • DOI: https://doi.org/10.1007/978-3-642-60268-9_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-62437-0

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