Abstract
We studied the contribution of Hsc70 to barotolerance by measuring barotolerance in logarithmic phase cells of the Hsc70 (ssb1 and ssb2) deletion mutant and the deletion mutant which carries the Hsc70 gene on the low copy number plasmid and high copy number plasmid. The deletion mutant strain showed higher thermotolerance and slightly lower barotolerance than that of the control strain. The mutant which has Hsc70 gene on multicopy plasmid showed more barotolerance than the strain which has the gene on low copy plasmid. These results suggest that Hsc70 contributes to barotolerance. We also compared the effect of Hspl04 and Hsc70 deletion to barotolerance at different temperatures (20-35 °C). The effect of Hsp l04 deficiency was significant with increase in temperature; however, that of Hsc 70 was significant at moderate temperatures. These results suggest Hsp l04 contributes to barotolerance at higher temperature and Hsc 70 at moderate temperature.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bett, K. E. and Cappi, J. B. (1965); Effect of pressure on the viscocity of water; Nature 207,620–621.
Hayashi, R. (1989); Use of high pressure to food processing and preservation; Use of High Pressure in Food, 1–30.
Iwahashi, H, Obuchi, K, Fujii, S. and Komatsu, Y. (1997); Barotolerance is dependent on both trehalose and heat shock protein 104 but is essentially different from thermotolerance in Saccharomyces cerevisiae; Lett. Appl. Microbiol., 25, 43–47.
in preparation.
Iwahashi, H., Obuchi, K., Fujii, S. and Komatsu, Y. (1997); Effect of temperature on the role of Hspl04 and trehalose in barotolerance of Saccharomyces cerevisiae; FEBS Letters, 416, 1–5.
Iwahashi, H., Yang, W. and Tanguay, R. M. (1995); Detection and expression of the 70 kDa heat shock protein ssblp at different temperatures in Saccharomyces cerevisiae; Biochem. Biophys. Res. Com., 213, 484–48.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Iwahashi, H., Nwaka, S., Obuchi, K., Komatsu, Y. (1999). Hsc70 Is Not Essential for Thermotolerance but for Barotolerance. In: Ludwig, H. (eds) Advances in High Pressure Bioscience and Biotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60196-5_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-60196-5_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-64300-2
Online ISBN: 978-3-642-60196-5
eBook Packages: Springer Book Archive