Abstract

The elucidation of the mechanism of enzyme reaction generally requires knowledge of the maximum values of the thermodynamic parameters. High hydrostatic pressure associated with temperature can lead to such data. A description of these approaches is given together with applications where data treatment can be achieved using the general “induced-fit” theory which implies that the binding of substrate to enzyme is a two-step process.

Keywords

Entropy Peroxide Ethyl Enthalpy DMSO 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • C. Balny
    • 1
  1. 1.INSERM U 128, IFR 24, CNRSMontpellier Cedex 5France

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