The elucidation of the mechanism of enzyme reaction generally requires knowledge of the maximum values of the thermodynamic parameters. High hydrostatic pressure associated with temperature can lead to such data. A description of these approaches is given together with applications where data treatment can be achieved using the general “induced-fit” theory which implies that the binding of substrate to enzyme is a two-step process.


Reversed Micelle High Hydrostatic Pressure Transition State Theory Subzero Temperature Transition State Theory 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • C. Balny
    • 1
  1. 1.INSERM U 128, IFR 24, CNRSMontpellier Cedex 5France

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