Peritoneal B-1 Cells Switch in vivo to IgA and these IgA Antibodies can bind to Bacteria of the Normal Intestinal Microflora
A prominent feature of the mucosal immune system is formed by IgA secreting cells located in the lamina propria, immediately beneath the epithelium. In the intestine of the mouse at least 15 million cells secrete IgA into the surrounding connective tissue . Subsequently, secretory IgA is transported across the single layer of epithelial cells and pumped into the gut lumen. Here, IgA can bind to food antigens and to the numerous microorganisms (in humans about 1011 bacteria per gram colon contents!). This interaction may help to prevent invasion and infection of the body with microbes, e.g. by preventing binding to the mucus layer and the epithelial cells (‘immune exclusion’).
KeywordsSCID Mouse Fecal Bacterium Isotype Switching Germline Transcript Immune Exclusion
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- 4.Kroese FGM, Kantor AB, and Herzenberg LA. (1994). The role of B-l cells (Ly-1 B cells) in mucosal immune responses. In: Ogra PL, Lamm ME, McGhee JR, Mestecky J, Strober W, Bienenstock J (Eds) Handbook of Mucosal Immunology. Academic Press, Inc. San Diego, pp 217 – 224.Google Scholar
- 9.Wetzel GD. (1990). Interleukin 5 regulation of peritoneal B-cell proliferation and antibody secretion. Scand. J. Immunol. 31: 91 – 101Google Scholar
- 15.Bos NA, Bun JCAM, Popma SH, Cebra ER, Deenen GJ, Cammen MJFvd, Kroese FGM, and Cebra JJ. (1996). Monoclonal Immunoglobulin A derived from peritoneal B cells is encoded by both germ line and somatically mutated VH genes and reactive with commensal bacteria. Inf Immun 64: 616 – 623.Google Scholar
- 16.Quan CP, Berneman A, Pires R, Avrameas S, and Bouvet JP. (1997). Natural polyreactive secretory immunoglobulin A autoantibodies as a possible barrier to infection in humans. Inf Immun 65: 3997 – 4004.Google Scholar
- 17.Marcotte H, and Lavoie MC. (1996). No apparent influence of immuno-globulins on indigenous oral and intestinal microbiota of mice. Inf Immun 64: 4694 – 4699.Google Scholar
- 20.Bos NA, Rijsemus AN, Van de Werf M, Schut F, and Welling GW. 1997. Usage of 16S rRNA in situ hybridisation to study bacterial population dynamics in host-flora interactions. In: Hashimoto K, Sakakibara B, Tazume S, Shimizu K (Ed) Germfree life and its ramifications. XIIISG Publishing Committee, Shiozawa, pp 231 – 235.Google Scholar
- 22.Michetti P, Mahan MJ, Slauch JM, Mekalanos JJ, and Neutra MR. (1992). Monoclonal secretory immunoglobulin A protects mice against oral challlenge with the invasive pathogen Salmonella typhimurium. Inf Immun 60: 1786 – 1792.Google Scholar
- 23.Apter FM, Lenger WI, Finkelstein RA, Mekalanos JJ, and Neutra MR. (1993). Monoclonal immunoglobulin a antibodies directed against cholera toxin prevent the toxin-induced chloride secretory response and block toxin binding to intestinal epithelial cells in vitro. Inf Immun 61: 5271 – 5278.Google Scholar
- 25.Shroff KE, Meslin K, and Cebra JJ. (1995). Commensal enteric bacteria engender a self- limiting humoral mucosal immune response while permanently colonizing the gut. Inf Immun 63: 3904 – 3913.Google Scholar