Fetal Liver Organ Cultures as a Tool to Study Selection Processes During B Cell Development
Recent studies concerning B cell development in the mouse embryo have indicated that the first cells committed to the B cell lineage arise in the para-aortic splanchnopleura at a time prior to the hematopoietic colonisation of the fetal liver . The B lymphoid potential of such cells was revealed by in vivo transfer experiments, and has recently been confirmed by analysis in vitro . Whether and in what way the mature B cell progeny of such progenitors contribute to the B cell repertoire in the developing embryo is less clear. This also raises the question of the quantitative and qualitative contribution of splanchnopleura and fetal liver-derived cells to the pool of developing B cells.
KeywordsFetal Liver Primary Lymphoid Organ Fetal Thymic Organ Culture Surrogate Light Chain preB Cell
Unable to display preview. Download preview PDF.
- 3.Owen JJT, Cooper MD, and Raff MC. (1974) In vitro generation of B lymphocytes in mouse fetal liver, a mamalian “bursa equivalent“. Nature 249: 361–363Google Scholar
- 4.Raff MC, Owen JJ, Cooper MD, Lawton A, Megson M, and Gathings WE. (1975) Differences in susceptibility of mature and immature mouse B lymphocytes to anti-immunoglobulin-induced immunoglobulin suppression in vitro. Possible implications for B-cell tolerance to self. J Exp Med 142: 1052 – 1064PubMedCrossRefGoogle Scholar
- 10.Ceredig R. (1988) Differentiation potential of 14-day fetal mouse thymocytes in organ culture: Analysis of CD4/CD8-defined single positive and double negative cells. J Immunol 141: 355– 362Google Scholar