Fetal Liver Organ Cultures as a Tool to Study Selection Processes During B Cell Development

  • R. Ceredig
  • A. G. Rolink
  • F. Melchers
  • J. Andersson
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 246)


Recent studies concerning B cell development in the mouse embryo have indicated that the first cells committed to the B cell lineage arise in the para-aortic splanchnopleura at a time prior to the hematopoietic colonisation of the fetal liver [1]. The B lymphoid potential of such cells was revealed by in vivo transfer experiments, and has recently been confirmed by analysis in vitro [2]. Whether and in what way the mature B cell progeny of such progenitors contribute to the B cell repertoire in the developing embryo is less clear. This also raises the question of the quantitative and qualitative contribution of splanchnopleura and fetal liver-derived cells to the pool of developing B cells.


Fetal Liver Primary Lymphoid Organ Fetal Thymic Organ Culture Surrogate Light Chain preB Cell 
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  1. 1.
    . Cumano A, Dieterlen-Lievre F, and Godin I. (1996) Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura. Cell 86: 907 – 916PubMedCrossRefGoogle Scholar
  2. 2.
    Nourrit F, Doyen N, Kourilsky P, Rougeon F, and Cumano A. (1998) Extensive junctional diversity of Ig heavy chain rearrangements generated in the progeny of single fetal multipotent hematopoietic cells in the absence of selection. J Immunol 160: 4254 – 4261PubMedGoogle Scholar
  3. 3.
    Owen JJT, Cooper MD, and Raff MC. (1974) In vitro generation of B lymphocytes in mouse fetal liver, a mamalian “bursa equivalent“. Nature 249: 361–363Google Scholar
  4. 4.
    Raff MC, Owen JJ, Cooper MD, Lawton A, Megson M, and Gathings WE. (1975) Differences in susceptibility of mature and immature mouse B lymphocytes to anti-immunoglobulin-induced immunoglobulin suppression in vitro. Possible implications for B-cell tolerance to self. J Exp Med 142: 1052 – 1064PubMedCrossRefGoogle Scholar
  5. 5.
    Raff MC, Megson M, Owen JJT, and Cooper MD. (1976) Early production of intracellular IgM by B lymphocyte precursors in mouse. Nature 259: 224 – 226PubMedCrossRefGoogle Scholar
  6. 6.
    Melchers F, Rolink A, Grawunder U, Winkler TH, Karasuyama H, Ghia P, and Andersson J. (1995) Positive and negative selection events during B lymphopoiesis. Curr Opin Immunol 7: 214 – 227PubMedCrossRefGoogle Scholar
  7. 7.
    Whitlock CA, and Witte ON. (1982) Long-term culture of B lymphocytes and their precursors from murine bone marrow. Proc Natl Acad Sci USA 79: 3608 – 3612PubMedCrossRefGoogle Scholar
  8. 8.
    Hayashi S, Kunisada T, Ogawa M, Sudo T, Kodama H, Suda T, Nishikawa S, and Nishikawa S. (1990) Stepwise progression of B lineage differentiation supported by interleukin 7 and other stromal cell molecules. J Exp Med 171: 1683 – 1695PubMedCrossRefGoogle Scholar
  9. 9.
    Rolink A, Kudo A, Karasuyama H, Kikuchi Y, and Melchers F. (1991) Long-term proliferating early preB cell lines and clones with the potential to develop to surface-Ig positive mitogen- reactive B cells “in vitro” and “in vivo”. EMBO J 10: 327 – 336PubMedGoogle Scholar
  10. 10.
    Ceredig R. (1988) Differentiation potential of 14-day fetal mouse thymocytes in organ culture: Analysis of CD4/CD8-defined single positive and double negative cells. J Immunol 141: 355– 362Google Scholar
  11. 11.
    Jenkinson EJ, and Anderson G. (1994) Fetal thymic organ cultures. Curr Opin Immunol 6: 293 – 297PubMedCrossRefGoogle Scholar
  12. 12.
    Bevan MJ. (1997) In thymic selection, peptide diversity gives and takes away. Immunity 7: 175 – 178PubMedCrossRefGoogle Scholar
  13. 13.
    Ceredig R, ten Boekel E, Rolink A, Melchers F, and Andersson J. (1998) Fetal liver organ cultures allow the proliferative expansion of pre-B receptor-expressing pre-B-II cells and the differentiation of immature and mature B cells in vitro. Int Immunol 10: 49 – 59PubMedCrossRefGoogle Scholar
  14. 14.
    Owen JJT, Raff MC, and Cooper MD. (1975) Studies on the generation of B lymphocytes in the mouse embryo. Eur J Immunol 5: 468 – 473CrossRefGoogle Scholar
  15. 15.
    Rolink A, Haasner D, Nishikawa SI, and Melchers F. (1993) Changes in frequencies of clonable preB cells during life in different lymphoid organs of mice. Blood 81: 2290 – 2300PubMedGoogle Scholar
  16. 16.
    Sudo T, Nishikawa S, Ohno N, Akiyama N, Tamakoshi M, Yoshida H, and Nishikawa S. (1993) Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A 90: 9125 – 9129PubMedCrossRefGoogle Scholar
  17. 17.
    Kohler G, and Milstein C. (1976) Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol 6: 511 – 519PubMedCrossRefGoogle Scholar
  18. 18.
    Andersson J, Melchers F, and Rolink A. (1995) Stimulation by T cell independent antigens can relieve the arrest of differentiation of immature auto-reactive B cells in the bone marrow. Scand J Immunol 42: 21 – 33PubMedCrossRefGoogle Scholar
  19. 19.
    Dray S. (1962) Effect of maternal isoantibodies on the quantitative expression of two allelic genes controlling g-globulin allotypic specificities. Nature 195: 677 – 680PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • R. Ceredig
    • 1
    • 2
  • A. G. Rolink
    • 1
  • F. Melchers
    • 1
  • J. Andersson
    • 1
  1. 1.The Basel Institute for ImmunologyBaselSwitzerland
  2. 2.Université Louis PasteurStrasbourgFrance

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