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Diagnosis of Helicobacter pylori Infection

  • Chapter
Book cover Gastroduodenal Disease and Helicobacter pylori

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 241))

Abstract

Success in diagnosing a disease depends to a large extent upon the choice of diagnostic techniques. Nowhere is this more obvious than in the case of gastroduodenal infection withHelicobacter pylori. H. pylori had been observed in the gastric mucosa by several independent investigators since the beginning of the twentieth century (Krienitz 1906; Luger and Neuberger 1921; Doenges 1939; Freedberg and Barron 1940), yet its existance was often doubted by the experts of the time. In 1954 Palmer reported a large study of more than 1000 patients undergoing suction biopsies of their stomachs.Palmer had specifically looked for the “spirochetes” observed by others, but found none and concluded that these organisms were just “simple contamination of the mucosal surface by swallowed spirochetes.” Palmer missed the opportunity to discover H. pylori by using an inappropriate diagnostic test. He stained his biopsies with the commonly used H&E stain. This stain is excellent for displaying tissue morphology but can be a relatively poor stain for visualizing H. pylori. Twenty-five years later Warren in Australia was studying gastric mucosa using a Warthin-Starry silver stain (Marshall 1989). This stain shows the bacteria very well, and soon he and Marshall surprised the scientific community by reporting the presence of “unidentified curved bacilli on gastric epithelium in active chronic gastritis” (Warren and Marshall 1983).

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  1. Department of Internal Medicine, College of Medicine, Texas A&M University, Central Texas Veterans Health Care System, Temple, Texas, USA

    T. U. Westblom & B. D. Bhatt

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  1. T. U. Westblom
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  1. Medical Service (111), Central Texas Veterans Health Care System, Texas A&M University, College of Medicine, 1901 S. First Street, Temple, TX, 76504, USA

    T. Ulf Westblom M.D. (Chief) (Chief)

  2. Department of Pediatrics, Division of Pediatric Gastroenterology, Rainbow Babies and Children Hospital, Case Western Reserve University, 2074 Abington Road, Cleveland, OH, 44106, USA

    Steven J. Czinn M.D.

  3. Institute of Pathology, Case Western Reserve University, Biomedical Research Building, Rm. 919, 10900 Euclid Avenue, Cleveland, OH, 44106-4943, USA

    John G. Nedrud Ph.D. (Associate Professor) (Associate Professor)

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© 1999 Springer-Verlag Berlin Heidelberg

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Westblom, T.U., Bhatt, B.D. (1999). Diagnosis of Helicobacter pylori Infection. In: Westblom, T.U., Czinn, S.J., Nedrud, J.G. (eds) Gastroduodenal Disease and Helicobacter pylori . Current Topics in Microbiology and Immunology, vol 241. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-60013-5_11

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  • DOI: https://doi.org/10.1007/978-3-642-60013-5_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64221-0

  • Online ISBN: 978-3-642-60013-5

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