Protease-Catalyzed Peptide Synthesis
Peptides play a fundamental role in the function of living systems. Since the demand for peptides is enormous, classical chemical synthesis in solution, solid-phase synthesis and recombinant techniques belong to the most important methods of peptide synthesis. Unfortunately, neither of these methods is completely free of drawbacks. Especially chemical synthesis of peptides often suffers from problems such as time-consuming side-chain protection/deprotection requirements, racemization and other side-reactions. Furthermore, in recombinant DNA methods, the incorporation of unnatural amino acids is limited in preparative scale. For these reasons, the use of suitable peptide ligases should provide an attractive alternative by combining the flexibility of chemical strategies with the advantages of the regio- and stereospecific enzymatic reactions (Jakubke 1987; Bongers and Heimer 1994; Jakubke 1994; Bordusa et al. 1997).
KeywordsPeptide Synthesis Acyl Donor Preparative Scale Acyl Transfer Peptide Bond Formation
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- Jakubke HD (1987) Enzymatic peptide synthesis. In: Udendriend S, Meienhofer J (eds) The Peptides: Analysis, Synthesis, Biology, vol 9. Academic Press, New York, pp 103–165Google Scholar
- Jakubke HD (1994) Protease-catalyzed peptide synthesis: basic principles, new synthesis strategies and medium engineering. J Chin Chem Soc 41:355–370Google Scholar
- Kemp DS (1979) Racemization in peptide synthesis. In: Gross E, Meienhofer J (eds) The Peptides: Analysis, Synthesis, Biology, vol 1. Academic Press, New York, pp 315–383Google Scholar
- Morihara K, Oka K (1977) Alpha-chymotrypsin as the catalyst for peptide synthesis. In: Nakajima H (ed) Peptide Chemistry 1976. Protein Research Foundation, Osaka, pp 9–16Google Scholar
- Schellenberger V, Görner A, Könnecke A, Jakubke HD (1991) Protease-catalyzed peptide synthesis: Prevention of side reactions in kinetically controlled reactions. Peptide Res 4:265–269Google Scholar
- Ullmann G, Jakubke HD (1993) Frozen aqueous systems: New efficient reaction media for enzyme-catalyzed peptide bond formation. In: Schneider CH, Eberle AN (eds) Peptides 1992. ESCOM Sci Publ, pp 36–37Google Scholar