Electrophoresis of DNA and RNA Fragments
Characterization of DNA and RNA samples usually involves electrophoretic analysis. This chapter reviews basic principles and applications of electrophoresis, distinguishing between single field and pulsed field techniques. Also included is a review of the various matrices commonly used in molecular biology (agarose, acrylamide, Hydro-Link™). Methods of purification, detection and identification are discussed.
KeywordsBromphenol Blue Xylene Cyanol Ethidium Bromide Solution School ofMedicine Freeze Tube
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- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, and Struhl K (eds) (1987) Current Protocols in Molecular Biology. Wiley InterscienceGoogle Scholar
- Sambrook J, Fritsch EF, and Maniatis T (1989) Molecular cloning — A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar