Electrophoresis of DNA and RNA Fragments

Part of the Springer Lab Manual book series (SLM)

Abstract

Characterization of DNA and RNA samples usually involves electrophoretic analysis. This chapter reviews basic principles and applications of electrophoresis, distinguishing between single field and pulsed field techniques. Also included is a review of the various matrices commonly used in molecular biology (agarose, acrylamide, Hydro-Link™). Methods of purification, detection and identification are discussed.

Keywords

Formaldehyde Migration Microwave EDTA Agarose 

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References

  1. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, and Struhl K (eds) (1987) Current Protocols in Molecular Biology. Wiley InterscienceGoogle Scholar
  2. Sambrook J, Fritsch EF, and Maniatis T (1989) Molecular cloning — A laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  3. Church GM and Gilbert W (1984) Proc Natl Acad Sci USA 81:1991–1995PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  1. 1.School of Medicine, Division of NephrologyThe Johns Hopkins UniversityBaltimoreUSA

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