Microfluidic Devices on Polymer Substrates for Bioanalytical Applications
Development of capabilities to miniaturize analytical devices and components offers a number of potential benefits. Among these are the ability to reduce sample sizes, development of low cost, single-use disposable devices, and improved device portability. Extensive work has been done on producing such microanalytical systems on silicon or glass substrates using processes commonly employed in electronic chip manufacturing [1–2]. For many analytical applications, however, common polymeric materials provide acceptable substrates from which to produce components or complete analytical systems [3–4]. The low flow rate characteristic of these microfluidic devices is compatible with the electrospray ionization/mass spectrometry (ESI-MS) [5–7]. In this paper, development of microfluidic analytical devices fabricated on polymer substrates using excimer laser micromachining technology will be described. These include a microfluidic motherboard, dual-stage microdialysis chip, and a micro capillary isoelectric focusing device. The applications of these micr ode vices for cleanup, fractionation, and separation of biological samples are discussed.
KeywordsMicrofluidic Device Excimer Laser Protein Mixture Pacific Northwest National Laboratory Sample Channel
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