Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting

  • M. Hausmann
  • C. Cremer
  • P. Popescu
  • D. Celeda
Part of the Principles and Practice book series (PRINCIPLES)

Abstract

Fluorescence activated analysis and sorting (“flow cytometry”) offers a successful method to characterise particles in a fast, statistically quantitative way (Melamed et al., 1990). Originally developed to analyse and sort cells (Bonner et al., 1972) it is nowadays a highly versatile tool for biomedical research and routine clinical diagnostics (Darzynkiewicz et Crissman, 1990). Flow cytometry was introduced into cytogenetics about 20 years ago (Gray et al., 1975; Stubblefield et al., 1975). “Flow cytogenetics” (Gray and Langlois, 1986; Cremer et al., 1989a) contributed significantly to medicine and molecular biology.

Keywords

Acetone Pyridine Syringe Trypsin Polystyrene 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • M. Hausmann
  • C. Cremer
  • P. Popescu
  • D. Celeda

There are no affiliations available

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