Transgenic Rice (Oryza sativa)

  • S. Yokoi
  • K. Toriyama
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 46)

Abstract

Rice (Oryza sativa L.) is one of the most important cereals in the world, and extensive studies have been carried out on tissue culture and transformation (see Hasezawa et al. 1989; Bajaj 1991; Uchimiya and Toriyama 1991). However, transformation of rice by Agrobacterium-mediated gene transfer has not been successful until recently, and gene transfer methods developed for rice were restricted to direct gene transfer into protoplasts or particle bombardment methods of direct DNA transfer into intact cells of embryogenic callus or suspension cells (Table 1).

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References

  1. Aldemita RR, Hodges TK (1996)Agrobacterium tumefaciens-mediated transformation of japonica and indica rice varieties. Planta 199:612–617.CrossRefGoogle Scholar
  2. An G, Ebert PR, Mitra A, Ha SB (1988) Binary vectors. In: Gelvin SB, Schilperoort RA (eds) Plant molecular biology manual. Kluwer, Dordrecht, pp A3/1–19.Google Scholar
  3. Bajaj YPS (ed) (1991) Rice. Biotechnology in agriculture and forestry, vol 14. Springer, Berlin Heidelberg New York.Google Scholar
  4. Battraw MJ, Hall TC (1990) Histochemical analysis of CaMV 35S promoter-ß-glucuronidase geneexpression in transgenic rice plants. Plant Mol Biol 15:527–538.Google Scholar
  5. Chan MT, Chang HH, Ho SL, Tong WF, Yu SM (1993) Agrobacterium-mediated production of transgenic rice plants expressing a chimeric a-amylase promoter/ß-glucuronidase gene. Plant Mol Biol 22:491–506.PubMedCrossRefGoogle Scholar
  6. Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated byAgrobacterium tumefaciens. Plant Physiol 115:971–980.PubMedGoogle Scholar
  7. Chilton MD, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW (1974)Agrobacterium tumefaciensDNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sei USA 71:3672–3676.CrossRefGoogle Scholar
  8. Chu CC, Wang CC, Sun CS, Hsu C, Yin C, Chu CY, Bi FY (1975) Establishment of an efficientmedium for anther culture of rice through comparative experiments on the nitrogen sources.Sei Sin 18:659–668.Google Scholar
  9. Cornejo M-J, Luth D, Blankenship KM, Anderson OD, Blechl AE (1993) Activity of a maize ubiq- uitin promoter in transgenic rice. Plant Mol Biol 23:567–581.PubMedCrossRefGoogle Scholar
  10. Dong J, Teng W, Buchholz WG, Hall TC (1996)Agrobacterium-mediated transformation of javanica rice. Molec Breed 2:267–276.CrossRefGoogle Scholar
  11. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158.PubMedCrossRefGoogle Scholar
  12. Hasezawa S, Baba A, Syoni K (1989) Protoplast culture and transformation studies on rice. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 9. Plant protoplasts and genetic engineering II. Springer, Berlin Heidelberg New York, pp 107–121.Google Scholar
  13. Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated byAgrobacteriumand sequence analysis of the boundaries of the T-DNA. Plant J 6:271–282.PubMedCrossRefGoogle Scholar
  14. Hiei Y, Komari T, Kubo T (1997) Transformation of rice mediated by Agrobacterium. Plant Mol Biol 35:205–218.PubMedCrossRefGoogle Scholar
  15. Hood EE, Helmer GL, Fraley RT, Chilton MD (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol 168:1291–1301.Google Scholar
  16. Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T (1996) High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol 14:745–750.PubMedCrossRefGoogle Scholar
  17. Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T (1996) Vectors carrying two separate T-DNAs for cotransformation of higher plants mediated by Agrobacterium tumefaciensand segregation of transformants free from selection markers. Plant J 10:165–174.Google Scholar
  18. Li Z, Murai N (1995) Agronomic trait evaluation of field-grown transgenic rice plants containing the hygromycin resistance gene and the maize Activatorelement. Plant Sei 108:219–227.CrossRefGoogle Scholar
  19. Li Z, Burow MD, Murai N (1990) High frequency generation of fertile transgenic rice plants after PEG-mediated protoplast transformation. Plant Mol Biol Rep 8:276–291.CrossRefGoogle Scholar
  20. Matsuki R, Onodera H, Yamauchi T, Uchimiya H (1989) Tissue-specific expression of the rolC promoter of the Ri plasmid in transgenic rice plants. Mol Gen Genet 220:12–16.CrossRefGoogle Scholar
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497.CrossRefGoogle Scholar
  22. Ohta S, Mita S, Hattori T, Nakamura K (1990) Construction and expression in tobacco of a ß-glucuronidase (GUS) reporter gene containing an intron within the coding sequence. Plant Cell Physiol 31:805–813.Google Scholar
  23. Park SH, Pinson SRM, Smith RH (1996) T-DNA integration into genomic DNA of rice following Agrobacteriuminoculation of isolated shoot apices. Plant Mol Biol 32:1135–1148.PubMedCrossRefGoogle Scholar
  24. Raineri DM, Bottino P, Gordon MP, Nester EW (1990) Agrobacterium-mediated transformation of rice (Oryza sativa L.). Bio/Technology 8:33–38.CrossRefGoogle Scholar
  25. Rashid H, Yokoi S, Toriyama K, Hinata K (1996) Transgenic plant production mediated by Agrobacterium in Indicarice. Plant Cell Rep 15:727–730.CrossRefGoogle Scholar
  26. Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Fertile transgenic rice plants regenerated from transformed protoplasts. Nature 338:274–276.CrossRefGoogle Scholar
  27. Tada Y, Sakamoto M, Fujiwara T (1990) Efficient gene introduction into rice by electroporation and analysis of transgenic plants: use of electroporation buffer lacking chloride ions. Theor Appl Genet 80:475–480.CrossRefGoogle Scholar
  28. Tada Y, Sakamoto M, Matsuoka M, Fujimura T (1991) Expression of a monocot LHCP promoter in transgenic rice. EMBO J 10:1803–1808.Google Scholar
  29. Tanaka A, Mita S, Ohta S, Kyozuka J, Shimamoto K, Nakamura K (1990) Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron. Nucleic Acids Res 18:6767–6770.PubMedCrossRefGoogle Scholar
  30. Terada R, Shimamoto K (1990) Expression of CaMV35S-GUS gene in transgenic rice plants. Mol Gen Genet 220:389–392.Google Scholar
  31. Terada R, Nakayama T, Iwabuchi M, Shimamoto K (1993) A wheat histone H3 promoter confers cell division-dependent and -independent expression of the gusA gene in transgenic rice plants. Plant J 3:241–252.Google Scholar
  32. Toki S (1997) Rapid and efficient Agrobacterium-mediated transformation in rice. Plant Mol Biol Rep 15:16–21.CrossRefGoogle Scholar
  33. Toki S, Takamatsu S, Nojiri C, Ooba S, Anzai H, Iwata M, Christensen AH, Quail PH, Uchimiya H (1992) Expression of a maize ubiquitin gene promoter-barchimeric gene in transgenic rice plants. Plant Physiol 100:1503–1507.PubMedCrossRefGoogle Scholar
  34. Toriyama K, Hinata K (1985) Cell suspension and protoplast culture in rice. Plant Sei 41:179–183.CrossRefGoogle Scholar
  35. Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Transgenic rice plants after direct gene transfer into protoplasts. Bio/Technology 6:1072–1074.CrossRefGoogle Scholar
  36. Uchimiya H, Toriyama K (1991) Transformation in rice. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 14. Rice. Springer, Berlin Heidelberg New York, pp 415–421.Google Scholar
  37. Yokoi S, Tsuchiya T, Toriyama K, Hinata K (1997) Tapetum-specific expression of the Osg6B promoter-ß-glucuronidase gene in transgenic rice. Plant Cell Rep 16:363–367.Google Scholar
  38. Yokoi S, Higashi S-I, Kishitani S, Murata N, Toriyama K (1998) Introduction of the cDNA for Arabidopsisglycerol-3-phosphate acyltransferase (GPAT) confers unsaturation of fatty acids and chilling tolerance of photosynthesis on rice. Mol Breed 4:269–275.CrossRefGoogle Scholar
  39. Zhang W, Wu R (1988) Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants. Theor Appl Genet 76:835–840.CrossRefGoogle Scholar
  40. Zheng Z, Sumi K, Tanaka K, Murai N (1995) The bean seed storage protein ß-phaseolin is synthesized, processed, and accumulated in the vacuolar type-II protein bodies of transgenic rice endosperm. Plant Physiol 109:777–786.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • S. Yokoi
    • 1
  • K. Toriyama
    • 1
  1. 1.Laboratory of Plant Breeding and Genetics, Faculty of AgricultureTohoku UniversitySendaiJapan

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