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Retroviral Transfer and Expression of Drug Resistance Genes in Hematopoietic Cells

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Transplantation in Hematology and Oncology

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Abstract

Genes conferring resistance to anticancer drugs are expressed in hematopoietic cells to increase the therapeutic index of chemotherapy and to establish a system for dominant selection of gene-modified cells in vivo. We have developed powerful retroviral vectors for this approach. These vectors are known as Friend mink cell focus-forming/murine embryonic stem cells virus hybrid vectors (FMEV), characterised by mediating high constitutive transgene expression in hematopoietic cells. In our recent work, we have elaborated a new generation of FMEV expressing dominant selection markers like multidrug resistance 1 (MDR1) alone or in combination with other genes at further increased efficiency and safety.

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Author information

Authors and Affiliations

  1. Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Martinistr. 52, D-20251, Hamburg, Germany

    C. Baum, M. Hildinger & W. Ostertag

Authors
  1. C. Baum
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  2. M. Hildinger
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  3. W. Ostertag
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Editor information

Editors and Affiliations

  1. Department of Internal Medicine Hematology/Oncology, University of Muenster, Albert-Schweitzer-Straße 33, 48129, Muenster, Germany

    W. E. Berdel (Prof. Dr. med.), Th. Büchner (Prof. Dr. med.), J. Kienast  & J. Van De Loo (Prof. Dr. med.) (Prof. Dr. med.),  (Prof. Dr. med.),  &  (Prof. Dr. med.)

  2. Department of Pediatrics, University of Münster, Albert-Schweitzer-Straße 33, 48129, Muenster, Germany

    H. Jürgens (Prof. Dr. med.), J. Ritter (Prof. Dr. med.) & J. Vormoor (Dr. med.) (Prof. Dr. med.),  (Prof. Dr. med.) &  (Dr. med.)

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© 2000 Springer-Verlag Berlin Heidelberg

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Baum, C., Hildinger, M., Ostertag, W. (2000). Retroviral Transfer and Expression of Drug Resistance Genes in Hematopoietic Cells. In: Berdel, W.E., et al. Transplantation in Hematology and Oncology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59592-9_34

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  • DOI: https://doi.org/10.1007/978-3-642-59592-9_34

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-64041-4

  • Online ISBN: 978-3-642-59592-9

  • eBook Packages: Springer Book Archive

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