Retroviral Transfer and Expression of Drug Resistance Genes in Hematopoietic Cells

  • C. Baum
  • M. Hildinger
  • W. Ostertag

Abstract

Genes conferring resistance to anticancer drugs are expressed in hematopoietic cells to increase the therapeutic index of chemotherapy and to establish a system for dominant selection of gene-modified cells in vivo. We have developed powerful retroviral vectors for this approach. These vectors are known as Friend mink cell focus-forming/murine embryonic stem cells virus hybrid vectors (FMEV), characterised by mediating high constitutive transgene expression in hematopoietic cells. In our recent work, we have elaborated a new generation of FMEV expressing dominant selection markers like multidrug resistance 1 (MDR1) alone or in combination with other genes at further increased efficiency and safety.

Keywords

Toxicity Leukemia Recombination Aldehyde Sarcoma 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • C. Baum
    • 1
  • M. Hildinger
    • 1
  • W. Ostertag
    • 1
  1. 1.Heinrich-Pette-Institut für Experimentelle Virologie und ImmunologieHamburgGermany

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