Retroviral Transfer and Expression of Drug Resistance Genes in Hematopoietic Cells
Genes conferring resistance to anticancer drugs are expressed in hematopoietic cells to increase the therapeutic index of chemotherapy and to establish a system for dominant selection of gene-modified cells in vivo. We have developed powerful retroviral vectors for this approach. These vectors are known as Friend mink cell focus-forming/murine embryonic stem cells virus hybrid vectors (FMEV), characterised by mediating high constitutive transgene expression in hematopoietic cells. In our recent work, we have elaborated a new generation of FMEV expressing dominant selection markers like multidrug resistance 1 (MDR1) alone or in combination with other genes at further increased efficiency and safety.
KeywordsHematopoietic Cell Long Terminal Repeat Internal Ribosomal Entry Site Alternative Splice Site Cancer Drug Resistance
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