Skip to main content

Microbiological Aspects

  • Chapter
Madkour’s Brucellosis

Abstract

The genus Brucella consists of a small coherent group of very closely related bacteria. Largely on the basis of preferred natural host, which shows a fairly close correlation with phage sensitivity and ability to oxidise certain substrates, the genus has been divided into six species, three of which have been subdivided into biovars(Corbel and Brinley Morgan 1984). On genetic grounds there is little justification for the recognition of separate species within the genus as no evidence of subdivision has been detected by DNA-DNA hybridisation. Indeed, on this basis it has been suggested that the classification should be amended to recognise that the genus comprises a single species, Brucella melitensis, with the currently recognised nomen species identified as its biovars (Verger et al. 1985). However, recent studies on the genomic structure have indicated that the current types are probably derived from an ancestral form most closely resembling B. suis biovar 3 (Jumas-Bilak et al. 1998). Thus on genetic grounds the various members of the genus should be classified as variants of B. suis. However, no system of nomenclature based on genetic structure has yet been proposed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Advisory Committee on Dangerous Pathogens (1993) Categorisationof biological agents according to hazard and categories of containment. HSE Books, Sudbury.

    Google Scholar 

  • Allardet Servent A., Bourg G., Ramuz M., Pages M., Bellis M., Roizes G. (1988) DNA polymorphism in strains of the genus Brucella. Journal of Bacteriology, 170, 4603–4607.

    PubMed  CAS  Google Scholar 

  • Anon. (1998) The development of new/improved brucellosis vaccines: report of a WHO Meeting. WHO/EMC/ZDI/98. 14.

    Google Scholar 

  • World Health Organisation, Geneva.

    Google Scholar 

  • Bachrach, G., Banai M., Bardenstein S., Hoida G., Genizi A., Bercovier H. (1994) Brucella ribosomal protein L7/L12 is a major component in the antigenicity of Brucellin INRA for delayed-type hypersensitivityin brucella-sensitized guinea pigs. Infection and Immunity, 62, 5361–5366.

    PubMed  CAS  Google Scholar 

  • Barham W., Church P., Brown J., Paparello S., (1993) Misidentification of Brucella species with use of rapid bacterial identification systems. Clinical Infectious Diseases, 17, 1068–1069.

    Article  PubMed  CAS  Google Scholar 

  • Brew S.D., Perett L.L., Stack J.A, MacMillan A.P., Staunton, N.J. (1999) Human exposure to Brucella recovered from a sea mammal. Veterinary Record, 144, 483.

    PubMed  CAS  Google Scholar 

  • Brodie J., Sinton, G.P. (1975) Fluid and solid media for isolation of Brucella abortus. Journal of Hygiene, Cambridge, 74, 359–367.

    Article  CAS  Google Scholar 

  • Broughton E.S., Jahans K.L. (1997) The differentiation of Brucella species by substrate specific tetrazolium reduction Veterinary Microbiology, 51, 253–271.

    Article  Google Scholar 

  • Bundle D.R., Cherwonogrodzky J.W., Caroff M., Perry M.B. (1987) The lipopolysaccharides of Brucella abortus and B. melitensis. Annales de Vlnstitut Pasteur/Microbiologie, 138, 92–98.

    Article  CAS  Google Scholar 

  • Castañeda, M.R. (1947) A practical method for routine blood cultures in brucellosis. Proceedings of the Society for Experimental Biology and Medicine, N.Y., 64, 114–115

    Google Scholar 

  • Collins, C.H. (1983) Laboratory-Acquired Infections. History, Incidence, Causes and Prevention, Butterworths, London

    Google Scholar 

  • Corbel, M.J. (1973) The direct fluorescent antibody test for detection of Brucella abortus in bovine abortion material. Journal of Hygiene, Cambridge, 71, 123–129

    Article  CAS  Google Scholar 

  • Corbel, M.J. (1985) Recent advances in the study of Brucella antigens and their serological cross-reactions. Veterinary Bulletin, 55, 927–942

    Google Scholar 

  • Corbel M.J. (1987) Brucella phages: Advances in the development of a reliable phage typing system for smooth and non-smooth Brucella isolates. Annales de l’Institut Pasteur/Microbiologie, 138, 70–75.

    Article  CAS  Google Scholar 

  • Corbel, M.J. and Thomas, E.L. (1983) The Brucella-phages: Their Properties, Charactisation and Applications, 2nd edn. Ministry of Agriculture, Fisheries and Food, London

    Google Scholar 

  • Corbel, M.J. and Brinley Morgan, W.J. (1984). Genus Brucella Meyer and Shaw 1920,173. In Bergey’s Manual of Systematic Bacteriology, Vol. 1 (eds N.R. Krieg and T.G. Holt ), Williams and Wilkins, Baltimore and London, pp. 377–388

    Google Scholar 

  • Corbel, M.J., Bracewell, C.D., Thomas, E.L. and Gill, K.P.W. (1979) Techniques in the identification and classification of Brucella species. In Identification Methods for Microbiologists, 2nd edn (eds F.A. Skinner and D.W. Lovelock ), Academic Press, London and New York, pp. 71–122

    Google Scholar 

  • De Ley, J., Mannheim, W., Segers, P. et al. (1983) Taxonomy of the genus Bordetella. In Les Bacilles ä Gram Negatif d’Interet Medical et en Sante Publique, Taxonomie, Identifications, Applications (ed. L.H. Leclerc ), INSERM, Paris, pp. 245–251

    Google Scholar 

  • Denoel P.A., Zygmunt M.S., Weynants V., Tibor A., Lichtfouse B., Briffeuil P., Limet J.N., Letesson J.-J. (1995) Cloning and sequencing of the bacterioferritin gene of Brucella melitensis 16M strain. FEBS Letters, 361, 238–242.

    Article  PubMed  CAS  Google Scholar 

  • FAO/WHO Expert Committee on Brucellosis. Sixth Report (1986) Technical Report Series No. 740. World Health Organisation, Geneva

    Google Scholar 

  • Farrell, I.D. (1974) The development of a new selective medium for the isolation of Brucella abortus from contaminated sources. Research in Veterinary Science, 16, 280–286.

    PubMed  CAS  Google Scholar 

  • Ficht T.A., Husseinen H.S., Derr J., Bearden S.W. (1996) Species-specific sequences at the omp 2 locus of Brucella type strains. International Journal of Systematic Bacteriology, 46, 329–331.

    Article  PubMed  CAS  Google Scholar 

  • Fox K.F., Fox A., Nagpal M., Steinberg P., Heroux K. (1998) Identification of Brucella by ribosomal-spacer-region PCR and differentiation of Brucella canis from other Brucella spp. pathogenic for humans by carbohydrate profiles. Journal of Clinical Microbiology, 36, 3217–3222.

    PubMed  CAS  Google Scholar 

  • Gedikoglu S., Helvaci H., Ozakun, C., Gokirmak, F., Kilicturgay, K. (1996) Detection of Brucella melitensis by BACTEC NR 730 and BACTEC 9120 systems. European Journal of Epidemiology 12, 649–650.

    Article  PubMed  CAS  Google Scholar 

  • Goldbaum FA, Velikovsky C A, Baldi P C, Mortl S, Bacher A, Fossati CA (1999) The 18-kDa cytoplasmic protein of Brucella species-an antigen useful for diagnosis-is a lumazine synthase. Journal of Medical Microbiology 48, 833–839.

    Article  PubMed  CAS  Google Scholar 

  • Hailing S M, Zehr E S (1990) Polymorphism in Brucella spp. due to highly repeated DNA. Journal of Bacteriology, 172, 6637–6640.

    Google Scholar 

  • Jumas-Bilak E, Michaux-Charachon S, Bourg G, O’Callaghan D, Ramuz M (1998) Differences in the chromosome number and genome rearrangements in the genus Brucella. Molecular Microbiology 27, 99–106.

    Article  PubMed  CAS  Google Scholar 

  • Kolman S, Maayan M C, Gotesman G, Rosenszayn L A, Wolach, B and Lang R (1991) Comparison of the BACTEC and lysis concentration methods for recovery of Brucella from clinical specimens. European Journal of Clinical Microbiology and Infectious Diseases, 10, 647–648.

    Article  CAS  Google Scholar 

  • Michaux-Charachon S, Bourg G, Jumas-Bilak E, Guigue-Talet P, Allardet-ServentA, O’Callaghan D, Ramuz M (1997) Genome structure and phylogeny in the genus Brucella. Journal of Bacteriology 179, 3244–3249.

    PubMed  CAS  Google Scholar 

  • Navas E, Guerrero A, Cobo J and Loza E (1992) Faster isolation of Brucella spp. from blood by Isolator compared with BACTEC NR. Diagnostic Moicrobiology and Infectious Disease 16, 79–81.

    Article  Google Scholar 

  • Sangari FJ, Aguero J (1994) Identification of Brucella abortus B19 strain by the detection of DNA polymorphism at the ery locus. Vaccine, 12, 435–438.

    Article  PubMed  CAS  Google Scholar 

  • Sulkin, SE and Pike, RM (1951) Laboratory-acquired infections. Journal of the American Medical Association, 147, 1740–1745

    PubMed  CAS  Google Scholar 

  • Verger, JM, Grimont, F, Grimont, PAD and Grayon M. (1985) Brucella; a monospecific genus as shown by deoxyribonucleic acid hybridization. International Journal of Systematic Bacteriology, 35, 292–295

    Google Scholar 

  • Verger, J M, Grayon M, Chaslus-Dancla E, Meurisse M, Lafont J P (1993) Conjugative transfer and in vitro/in vivo stability of the broad host range Inc PR751 plasmid in Brucella spp. Plasmid, 29, 142–146.

    Article  PubMed  CAS  Google Scholar 

  • World Health Organisation (1993) Laboratory Biosafety Manual. 2nd Edition. World Health Organisation, Geneva

    Google Scholar 

  • Yagupsky P (1999) Minireview. Detection of Brucellae in blood cultures. Journal of Clinical Microbiology 37, 3437–3442.

    PubMed  CAS  Google Scholar 

  • Yagupsky P, Peled N, Press J, Abramson O, Abu-Rashid M (1997) Comparison of BACTEC 9240 Peds Plus medium and Isolator 1.5 microbial tube for detection of Brucella melitensis from blood cultures. Journal of Clinical Microbiology 35, 1382–1384.

    PubMed  CAS  Google Scholar 

  • Yanagi, M, Yamasato, K (1993) Phylogenetic analysis of the family Rhizobiaceae and related bacteria by sequencing of 16 S rRNA gene using PCR and DNA sequencer. FEMS Microbiology Letters 107, 115–120.

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Rights and permissions

Reprints and permissions

Copyright information

© 2001 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Corbel, M.J. (2001). Microbiological Aspects. In: Madkour’s Brucellosis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59533-2_6

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-59533-2_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-67272-2

  • Online ISBN: 978-3-642-59533-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics