Abstract
Human viral load monitoring needs to be accurate and sensitive. Quantitative PCR assays are therefore useful. The development of real-time quantitative PCR on the LightCycler combines speed and accuracy. However, during PCR, non-specific amplification may compete with formation of specific products, leading to a reduced PCR efficiency and lower sensitivity. In this study, we demonstrate that an anti-Taq DNA polymerase antibody (TaqStart Antibody, Clontech) avoids the formation of primer-dimers and non-specific products in LightCycler PCR and improves the sensitivity of Epstein-Barr virus (EBV) and Human Herpesvirus type 8 (HHV8) DNA quantification.
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© 2001 Springer-Verlag Berlin Heidelberg
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Brengel-Pesce, K., Bargues, G., Morand, P., Seigneurin, JM. (2001). Use of TaqStart Antibody to Increase the Sensitivity of Herpesvirus Quantitative PCR on the LightCycler. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59524-0_7
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DOI: https://doi.org/10.1007/978-3-642-59524-0_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-66736-0
Online ISBN: 978-3-642-59524-0
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