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Development of Quantitative PCR Tests for the Detection of the Orthopox Virus Adsorption Protein Gene (ORF D8L) on the LightCycler

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Rapid Cycle Real-Time PCR

Abstract

The genus Orthopoxvirus (OPV) includes several species of well-known pathogens [13, 21]. Although the Variola and Alastrim viruses were eradicated by a worldwide compulsory vaccination with vaccinia virus, monkeypox and, especially in Europe, cowpox virus strains may cause diseases in humans. Human cowpox is a relatively rare zoonosis and cows are currently not known to be involved. In recent years, virus strains have drawn the attention of the medical profession by causing localized and generalized skin infections in humans [5, 6, 12, 14, 17, 24] as well as in cats [7, 10, 22] or zoo-animals like large felides or elephants [3, 4, 18, 25]. Usually, a low infectivity for healthy persons is observed with benign skin lesions. However, in immunocompromised and non-vaccinated individuals the infection can lead to severe diseases which may end lethally [8, 11, 12].

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Authors and Affiliations

  1. Senator-Gerauer-Str. 23, 85586, Poing, Germany

    Claus-Peter Czerny

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  1. Claus-Peter Czerny
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  2. Michaela Alex
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  3. Jana Pricelius
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  4. Christiane Zeller-Lue
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Editor information

Editors and Affiliations

  1. Institut für Immunologie, Ruprecht Karls-Universität Heidelberg, Im Neuenheimer Feld 305, 69120, Heidelberg, Germany

    Stefan Meuer

  2. Department of Pathology, University of Utah Medical School, 84132, Salt Lake City, UT, USA

    Carl Wittwer

  3. Sendai, Japan, 2-3-36 Ohgimachi, 983-0034, Miyagino-ku, Sendai, Japan

    Kan-Ichi Nakagawara

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© 2001 Springer-Verlag Berlin Heidelberg

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Czerny, CP., Alex, M., Pricelius, J., Zeller-Lue, C. (2001). Development of Quantitative PCR Tests for the Detection of the Orthopox Virus Adsorption Protein Gene (ORF D8L) on the LightCycler. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59524-0_40

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  • DOI: https://doi.org/10.1007/978-3-642-59524-0_40

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-66736-0

  • Online ISBN: 978-3-642-59524-0

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