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Quantification on the LightCycler

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Over the last 15 years, PCR has become an essential part of most laboratories involved in biomedical research. PCR amplification turns a few attograms of a specific fragment of nucleic acid (far too little to be analyzed directly or used in biochemical reactions) into as much as a microgram of DNA.


  • Human Immunodeficiency Virus Type
  • Fluorescence Resonance Energy Transfer
  • Noise Band
  • Amplification Curve
  • Threshold Line

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  • DOI: 10.1007/978-3-642-59524-0_3
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© 2001 Springer-Verlag Berlin Heidelberg

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Rasmussen, R. (2001). Quantification on the LightCycler. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg.

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-66736-0

  • Online ISBN: 978-3-642-59524-0

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