Quantification of Residual Tumor Cells in Monoclonal B-cell Lymphoma
With the advent of more effective therapeutic modalities, better methods to evaluate and quantify minimal residual disease (MRD) in patients with malignant lymphoma are needed. These methods should be highly sensitive in detecting very low amounts of malignant cells and should be specific for the malignant clone. In addition, these methods should allow the quantification of residual tumor cells. At present, the highest sensitivity is reached with PCR, allowing the detection of one malignant cell in 106 normal cells . The clone-specific hypervariable complementarity determining regions (CDRs) of the immunoglobulin heavy chain locus (IgVH) provide a useful marker for monitoring MRD in B-cell lymphoma during and after treatment [2, 3].
KeywordsLymphoma EDTA Leukemia Agarose MgCl
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