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Mutation Detection by Fluorescent Hybridization Probe Melting Curves

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Rapid Cycle Real-Time PCR

Abstract

Sequence analysis of nucleic acids is used in many fields of science and for a variety of purposes. Although direct sequencing remains the gold standard for initially characterizing a new region of DNA, it is not a practical solution for the routine analysis of a sequence once it is known. For this reason, molecular techniques that facilitate the analysis of variants in established sequence have widespread application and continue to be developed. Conventionally, the target is first amplified by PCR and then further processed for mutation detection. Recently developed instrumentation couples rapid PCR with fluorescent hybridization probes, allowing target amplification and product analysis to be done consecutively, without sample handling and within 30 min [1].

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Author information

Authors and Affiliations

  1. Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, 84132, USA

    Philip S. Bernard

Authors
  1. Philip S. Bernard
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  2. Astrid Reiser
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  3. Gregory H. Pritham
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Editor information

Editors and Affiliations

  1. Institut für Immunologie, Ruprecht Karls-Universität Heidelberg, Im Neuenheimer Feld 305, 69120, Heidelberg, Germany

    Stefan Meuer

  2. Department of Pathology, University of Utah Medical School, 84132, Salt Lake City, UT, USA

    Carl Wittwer

  3. Sendai, Japan, 2-3-36 Ohgimachi, 983-0034, Miyagino-ku, Sendai, Japan

    Kan-Ichi Nakagawara

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© 2001 Springer-Verlag Berlin Heidelberg

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Bernard, P.S., Reiser, A., Pritham, G.H. (2001). Mutation Detection by Fluorescent Hybridization Probe Melting Curves. In: Meuer, S., Wittwer, C., Nakagawara, KI. (eds) Rapid Cycle Real-Time PCR. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-59524-0_2

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  • DOI: https://doi.org/10.1007/978-3-642-59524-0_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-66736-0

  • Online ISBN: 978-3-642-59524-0

  • eBook Packages: Springer Book Archive

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