Genotyping of Angiotensin-Converting Enzyme and Angiotensinogen Polymorphisms with the LightCycler System
The renin-angiotensin system regulates blood pressure, and maintains electrolyte homeostasis in humans . In this system, angiotensinogen (AGT) is catalyzed by renin to form angiotensin I, which is then cleaved by angiotensin-converting enzyme (ACE) to yield angiotensin II, a potent vasopressor and effector on renal function . Recently, several studies have focused on the correlation between physiological disorders and the genetic variation of peptides in the reninangiotensin system. A specific mutation in the angiotensinogen gene was reported to be associated with essential hypertension [2,3]. Individuals with homozygous deletion alleles of ACE were reported to have a higher level of serum ACE and an increased risk of ischemic heart disease, sudden death, left ventricular hypertrophy, increased blood glucose levels, diabetic nephropathy, and premature death [4–7]. Detection of mutations in genes that constitute the renin-angiotensin system may be important in the prevention and control of disorders in the cardiovascular system, glucose metabolism, and urinary function.
KeywordsDeletion Allele Angiotensinogen Gene Increase Blood Glucose Level LightCycler System Reninangiotensin System
Unable to display preview. Download preview PDF.
- 1.Hardman JG, Limbird LE, Molinof PB, Ruddon RW, Gilman AG (1996) Renin and angiotensin. In: The pharmacological basis of therapeutics, (9th edn. McGraw-Hill, New York, pp 733–758Google Scholar
- 4.Cambien F, Poirier O, Lecerf L, Evans A, Cambou JP, Arveiler D, Luc G, Bird JM, Bara L, Ricard S, Tiret L, Amouyel P, Alhenc-Gelas F, Soubrier F (1992) Deletion polymorphysm in the gene for angiotensin-converting enzyme is a potent risk factor for myocardial infarction. Nature 359: 641–644PubMedCrossRefGoogle Scholar
- 9.Sambrook J, Fritsch EF, Maniatis T (1989) Appendix E: Commonly used techniques in molecular cloning. In: Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
- 11.Caplin BE, Rasmussen RP, Bernard PS, Wittwer CT (1999) Light CyclerTM Hybridization probes. Biochemica 1: 5–8Google Scholar
- 12.Wetmur JG (1995) Formation and structure of nucleic acid hybrids. In: Molecular biology and biotechnology: a comprehensive desk reference. Wiley-VCH, New York, pp 605–608Google Scholar
- 13.Blake RD (1995) Denaturation of DNA. In: Molecular biology and biotechnology: a comprehensive desk reference. Wiley-VCH, New York, pp 207–210Google Scholar