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In Vitro Protein Production for Structure Determination with the RTS System

  • Ho S. Cho
  • Jeffrey G. Pelton
  • Weiru Wang
  • Hisao Yokota
  • David E. Wemmer

Abstract

The goal of the Berkeley Structural Genomics Center is to determine the structures of all proteins encoded in the genomes of Mycoplasma pneumoniae and Mycoplasma genatalium or structural homologs from other organisms. To achieve this goal, we are working to develop high-throughput methods for protein expression for use in X-ray and NMR structure determination. In collaboration with Roche Molecular Biochemicals (Penzburg, Germany), we are investigating the usefulness of the Rapid Translation System (RTS) of in vitro protein production to generate target proteins in quantities suitable for structure determination. Very encouraging results have been obtained with the test protein phosphoserine phosphatase (PSP) from Methanococcus jannaschii (Mj).

Keywords

Mycoplasma Pneumoniae Roche Molecular Biochemical Phosphate Dihydrate Total Recording Time Hanging Drop Vapor Diffusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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    Wang W, Kim R, Jancarik J, Yokota H, Kim S-H (2001) Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 Å. Structure Fold Design 9:65–72CrossRefGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Ho S. Cho
    • 1
  • Jeffrey G. Pelton
    • 1
  • Weiru Wang
    • 2
  • Hisao Yokota
    • 1
  • David E. Wemmer
    • 1
    • 2
  1. 1.Physical Biosciences DivisionLawrence Berkeley National LaboratoryBerkeleyUSA
  2. 2.Department of ChemistryUniversity of CaliforniaBerkeleyUSA

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