Recombinant Expression of Functional Active MIA (Melanoma Inhibitory Activity) Protein for Mutation Analysis Using the RTS System

  • Anja Katrin Bosserhoff

Abstract

MIA (Melanoma Inhibitory Activity) is a protein secreted from human melanoma cell lines which was cloned recently (Blesch et al. 1994). MIA is expressed only in and secreted from malignant melanomas and chondrocytes (Bosserhoff et al. 1997, 1999). As MIA expression in chondrocytes is dependent on the differentiation status of the cells, it was also named CD-RAP (cartilage-derived retinoic acid-sensitive protein). Recent evidence indicates an important role in tumour progression and metastasis, as MIA mediates detachment of melanoma cells from extracellular matrix molecules such as fibronectin (Stoll et al. 2001). MIA expression levels closely parallel the capability of melanoma cells to form metastases in syngeneic animals (Guba et al. 2000; Bosserhoff et al. 2001). Further, increased MIA serum levels have been used as a reliable and clinically useful marker to detect and monitor metastatic disease in patients with malignant melanomas (Bosserhoff et al. 1997).

Keywords

HPLC Penicillin Disulfide Streptomycin BCIP 

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References

  1. Blesch A, Bosserhoff AK, Apfel R, Behl C, Hessdörfer B, Schmitt A, Jachimczak P, Lottspeich F, Schlingensiepen H, Buettner R, Bogdahn U (1994) Cloning of a novel malignant melanoma-derived growth regulatory protein, MIA. Cancer Res 54: 5695–5701Google Scholar
  2. Bosserhoff AK, Kaufmann M, Kaluza B, Bartke I, Zirngibl H, Hein R, Stolz W, Buettner R (1997) MIA, a novel serum marker for progression of malignant melanoma. Cancer Res 57: 3149–3153PubMedGoogle Scholar
  3. Bosserhoff AK, Moser M, Hein R, Landthaler M, Buettner R (1999) In situ expression patterns of melanoma-inhibiting activity ( MIA) in malignant melanomas and breast cancers. J Pathology 187: 446–454Google Scholar
  4. Bosserhoff AK, Echtenacher B, Hein R, Buettner R (2001) Functional role of MIA in regulating metastasis of malignant melanoma cells in an in vivo model. Melanoma Res 11: 417–421PubMedCrossRefGoogle Scholar
  5. Guba M, Bosserhoff AK, Steinbauer M, Abels C, Anthuber M, Buettner R, Jauch KW (2000) Over-expression of Melanoma Inhibitory Activity (MIA) enhances extravasation and metastasis of A-mel 3 melanoma cells in vivo. Brit J Cancer 83: 1216–1222PubMedCrossRefGoogle Scholar
  6. Jacob K, Bosserhoff AK, Wach F, Knuchel R, Klein EC, Hein R, Buettner R (1995) Characterization of selected strongly and weakly invasive sublines of a primary human melanoma cell line and isolation of subtractive cDNA clones. Int J Cancer 60: 668–675PubMedCrossRefGoogle Scholar
  7. Stoll R, Renner C, Ambrosius D, Golob M, Voelter W, Buettner R, Holak TA, Bosserhoff AK (2001) Structure and dynamics of human melanoma inhibitory activity (MIA) protein in solution: implications for metastasis. EMBO 20: 340–349CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • Anja Katrin Bosserhoff
    • 1
  1. 1.Institut für Pathologie, Molekulare PathologieUniversität RegensburgRegensburgGermany

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