Purification of Proteins for Sequencing
During the past decade, biochemical methods of protein purification have become so specialized and sophisticated that it is now difficult for the beginner, whether graduate student or specialist in another field, to follow all the minor but important details which lead to a successful procedure. It should always be kept in mind that there are many alternative ways to purify a particular protein. If a particular procedure is not working another must be tried.
KeywordsPurification Procedure Peripheral Membrane Protein Guanidinium Chloride Octyl Glucoside Inclusion Body Protein
Unable to display preview. Download preview PDF.
- Buse G, Steffens GJ, Steffens GCM, Meinecke K, Hensel S, Reumkens J (1986) Sequence analysis of complex membrane proteins (cytochrome c oxidase). In: B. Wittmann-Liebold et al. (eds) Advanced methods in protein microse-quence analysis. Springer, Berlin Heidelberg, New York, pp 340–351.CrossRefGoogle Scholar
- Harris ELV, Angal S (1990) Protein purification applications: a practical approach, IRL, Oxford.Google Scholar
- Harris ELV, Angal S (1989) Protein purification applications: a practical approach. IRL, Oxford.Google Scholar
- Marston FLA, Angal S, Lowe PA, Chan M, Hill CR (1988) Scale-up of the recovery and reactivation of recombinant proteins. Biochem Soc Tran. 16: 112–115.Google Scholar
- Marston FAO (1986) The purification of eukaryotic polypeptides synthesized in E. coli. Biochemical J 240:1–12.Google Scholar
- Schein CH (1989) Production of soluble recombinant proteins in bacteria. Biotechnology 7:1141–1149.Google Scholar
- Scopes RK (1994) Protein purification: principles and practice (3rd edn). Springer, Berlin Heidelberg New York, pp 1–380.Google Scholar