Purification of Proteins for Sequencing

  • D. A. Kyriakidis
Part of the Springer Lab Manual book series (SLM)

Abstract

During the past decade, biochemical methods of protein purification have become so specialized and sophisticated that it is now difficult for the beginner, whether graduate student or specialist in another field, to follow all the minor but important details which lead to a successful procedure. It should always be kept in mind that there are many alternative ways to purify a particular protein. If a particular procedure is not working another must be tried.

Keywords

HPLC Filtration Urea EDTA Glutathione 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Buse G, Steffens GJ, Steffens GCM, Meinecke K, Hensel S, Reumkens J (1986) Sequence analysis of complex membrane proteins (cytochrome c oxidase). In: B. Wittmann-Liebold et al. (eds) Advanced methods in protein microse-quence analysis. Springer, Berlin Heidelberg, New York, pp 340–351.CrossRefGoogle Scholar
  2. Enfords SO (1992) Control of in vivo proteolysis in the production of recombinant proteins. Trends in Biotechnol 10: 310–315.CrossRefGoogle Scholar
  3. Furth AJ (1980) Removing unbound detergent from hydrophobic proteins. Anal. Biochem 109, 207–215.PubMedCrossRefGoogle Scholar
  4. Harris ELV, Angal S (1990) Protein purification applications: a practical approach, IRL, Oxford.Google Scholar
  5. Harris ELV, Angal S (1989) Protein purification applications: a practical approach. IRL, Oxford.Google Scholar
  6. Hoess A, Arthur A K, Wanner G, Fanning E (1988) Recovery of soluble, biologically active recombinant proteins from total bacterial lysates using ion exchange resin. Biotechnology 6:1214–1217.CrossRefGoogle Scholar
  7. Marston FLA, Angal S, Lowe PA, Chan M, Hill CR (1988) Scale-up of the recovery and reactivation of recombinant proteins. Biochem Soc Tran. 16: 112–115.Google Scholar
  8. Marston FAO (1986) The purification of eukaryotic polypeptides synthesized in E. coli. Biochemical J 240:1–12.Google Scholar
  9. Penefsky HS, Tzagoloff A (1971) Extraction of water soluble enzymes and proteins from membranes. Methods in Enzymol 22:204–219.CrossRefGoogle Scholar
  10. Sassenfeld H M (1990) Engineering protein for purification. Trends Biotechnol 8: 88–93.PubMedCrossRefGoogle Scholar
  11. Schein CH (1989) Production of soluble recombinant proteins in bacteria. Biotechnology 7:1141–1149.Google Scholar
  12. Scopes RK (1994) Protein purification: principles and practice (3rd edn). Springer, Berlin Heidelberg New York, pp 1–380.Google Scholar
  13. van Kimmenade A, Bond M W, Schumacher JH, Laquoi C, Kastelein RA (1988) Expression, renaturation and purification of recombinant human inter-leukin 4 from E. coli. Eur J Biochem 173, 109–114PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • D. A. Kyriakidis

There are no affiliations available

Personalised recommendations