Model Plants for Nitrogen Fixation

Genetic and Molecular Tools for Analysing the Infection and Infestation of Clovers
  • B. G. Rolfe
  • M. A. Djordjevic
  • J. J. Weinman
  • J. W. Redmond
  • S. Natera
  • U. Mathesius
  • N. Guerreiro
  • C. Pittock
  • A. Morris
  • L. Roddam
  • J. de Majnik
  • E. Gärtner
  • J. McIver
Conference paper
Part of the NATO ASI Series book series (volume 39)

Abstract

Trifolium repens (white clover) and Trifolium subterraneum (subterranean clover) have proved to be good model systems for the investigation of Rhizobium symbiosis and plant defence systems. Considerable research has been done on the interaction of these clovers with Rhizobium leguminosarum bv. trifolii at the practical agricultural level. This work has now been supplemented with the application of many recent molecular techniques. Our research has investigated the interactions involved in development, defence and symbiosis of clovers. We have taken the dual approach of constructing transgenic plant tools and Proteome analysis to examine the activity of phytohormones and flavonoids as well as the roles of pathogenesis-related (PR) protein genes in these biological processes.

Keywords

Transgenic plants GUS reporter gene proteome legume root morphogenesis 

Selected References

  1. (1).
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    Weinman JJ, Djordjevic MA, Howles PA, Arioli T, Lewis-Henderson W, McIver J, Oakes M, Creaser EH and BG Rolfe (1991) The use of the genus Trifolium for the study of plant-microbe interactions. Hennecke H and DPS Verma (eds.) In: Advances in Molecular Genetics of Plant-Microbe Interactions, Vol 1, 168–173, Kluwer Academic Publishers, Dordrecht.Google Scholar
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    Weinman JJ, Djordjevic MA, Creaser EH, Lawson CGR, Broderick K, Mathesius U, Gärtner E, Pittock C, de Majnik J and BG Rolfe Preparing subterranean clovers for future biotechnology: molecular analysis of genes and proteins involved in stress and defence reactions and the construction of transgenic plants. (1995) Plant Protection Quarterly 10: 47–49.Google Scholar
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    Rolfe BG and J McIver (1996) Single-leaf bioassays for the study of root morphogenesis and Rhizobium-legume nodulation. Aust. J. Plant Physiol. 23: 271–283.CrossRefGoogle Scholar
  6. (6).
    Larkin P, Gibson JM, Mathesius U, Weinman JJ, Gärtner E, Hall E, Tanner G, Rolfe BG and MA Djordjevic (1996) Transgenic white clover. Studies with the auxin-responsive promoter, GH3, in root gravitropism and lateral root development. Transgenic Research 5: 1–11.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • B. G. Rolfe
    • 1
  • M. A. Djordjevic
    • 1
  • J. J. Weinman
    • 1
  • J. W. Redmond
    • 1
  • S. Natera
    • 1
  • U. Mathesius
    • 1
  • N. Guerreiro
    • 1
  • C. Pittock
    • 1
  • A. Morris
    • 1
  • L. Roddam
    • 1
  • J. de Majnik
    • 1
  • E. Gärtner
    • 1
  • J. McIver
    • 1
  1. 1.Plant Microbe Interaction Group, Research School of Biological SciencesAustralian National UniversityCanberraAustralia

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