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Oenothera Species (Evening Primrose): In Vitro Regeneration, Production of Flavonoids, Fatty Acids, and Other Secondary Metabolites

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Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 41)

Abstract

Species of the genus Oenothera L. (Onagra Miller) from the family Onagraceae are characteristic of America, the homeland of species acclimated in Europe (Szafer and Pawłowski 1959; Raven 1968). The American flora has the most numerous representatives; plants of these species can be found in natural localities (Rickett 1970, 1971), or they are grown as decorative plants with white, pink to reddish purple, or mostly bright yellow flowers (Bailey 1950; Encke 1960). A few species are also found in Russia (Shishkin and Bobrow 1949; Grossgejm 1962). At present, the genus Oenothera is believed to be distributed throughout the world with the exception of Antarctica (Rostański 1992).

Keywords

Multiple Shoot Callus Tissue Hydrolyzable Tannin Gamma Linolenic Acid Oenothera Biennis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Asanaka M, Kurimura T, Koshiura R, Okuda T, Mori M, Yokoi H (1988) Abstr, 4th Int Conf on Immunopharmacology, Osaka p 47Google Scholar
  2. Averett JE, Huang S, Wagner WL (1987) Flavonoid analysis of Oenothera speciosa (Onagraceae). Southwest Nat 32:117–120CrossRefGoogle Scholar
  3. Averett JE, Huang S, Wagner WL (1988) Flavonoid survey of Oenothera (Onagraceae): Sects. Gauropsis, Hartmannia, Kneiffia, Paradoxus and Xylopleurum. Am J Bot 75:476–483CrossRefGoogle Scholar
  4. Averett JE, Wagner WL, Huang S (1991) The flavonoids of Oenothera Sect. Megapterium (Onagraceae). Biochem Syst Ecol 19:87–89CrossRefGoogle Scholar
  5. Bailey LH (1950) The standard cyclopedia of horticulture. Macmillan, New York, pp 2328–2332Google Scholar
  6. Bajaj YPS, Furmanowa M, Olszowska O (1988) Biotechnology of the micropropagation of medicinal and aromatic plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 60–103Google Scholar
  7. Bartl S (1975) Die Gerbstoffe in der Gattung Oenothera. Dissertation, Universität Saarbrücken, SaarbrückenGoogle Scholar
  8. Biagi PL, Bordoni A, Hrelia S, Celadon M, Ricci GP, Cannella V, Patrizia A, Specchia F, Masi M (1994) The effect of gamma-linolenic acid on clinical status, red cell fatty acid composition and membrane microviscosity in infants with atopic dermatitis. Drugs Expt Clin Res 20:77–84Google Scholar
  9. Chenoy R, Hussain S, Tayob Y, O’Brien PMS, Moss MY, Morse PF (1994) Effect of oral gamolenic acid from evening primrose oil on menopausal flushing. Br Med J 308:501–503CrossRefGoogle Scholar
  10. Cohen Z, Vonshak A, Richmond A (1987) Fatty acid composition of Spirulina strains grown under various environmental conditions. Phytochemistry 26:2255–2258CrossRefGoogle Scholar
  11. Cohen Z, Norman HA, Heimer YM (1993) Potential use of substituted pyridazinones for selecting polyunsaturated fatty acid over-producing cell lines of algae. Phytochemistry 32:259–264CrossRefGoogle Scholar
  12. Corthout J, Pieters LA, Claeys M, Vanden Berghe DA, Vlietinck AJ (1991) Antiviral ellagitannins from Spondias mombin. Phytochemistry 30:1129–1130CrossRefGoogle Scholar
  13. Dickmann FA (1948) Oenothera biennis L. ais Ölpflanze. Pharmazie 3:275–276Google Scholar
  14. Duczmal KW, Ratajczak K, Ratajczak KW (1993) Zawartość kwasu γ-linolenowego w oleju z nasion trzech gatunków rodzaju Oenothera L. Herba Pol 39:179–186Google Scholar
  15. Encke F (1960) Pareys Blumengärtnerei Bd 2. Parey, Berlin, pp 197–199Google Scholar
  16. Fukuchi K, Sakagami H, Okuda T, Hatano T, Tanuma S, Kitajima K, Inoue Y, Inoue S, Ichikawa S, Nonoyama M, Konno K (1989) Inhibition of Herpes simplex virus infection by tannins and related compounds. Antiviral Res 1,1:285–298CrossRefGoogle Scholar
  17. Grossgejm AA (1962) Flora Kawkaza vol 6. Akademy of Sciences USSR Moskow pp 247–248Google Scholar
  18. Harborne JB (1991) Phytochemical methods. Chapman and Hall, LondonGoogle Scholar
  19. Harborne JB, Baxter H (1993) (eds) Phytochemical dictionary. A handbook of bioactive compounds from plants. Taylor and Francis, LondonGoogle Scholar
  20. Harte C (1994) Oenothera: contributions of a plant to biology. Monographs on theoretical and applied genetics, vol 20. Springer, Berlin Heidelberg New YorkGoogle Scholar
  21. Hatano T, Yasuhara T, Matsuda M, Yazaki K, Yoshida T, Okuda T (1989) Oenothein B, a dimeric hydrolyzable tannin of cyclic structure. Chem Pharm Bull 37:2269–2271CrossRefGoogle Scholar
  22. Hatano T, Yasuhara T, Matsuda M et al. (1990) Oenothein B, a dimeric, hydrolysable tannin with macrocyclic stucture, and accompanying tannins from Oenothera erythrosepala. J Chem Soc Perkin Trans 1, 1990:2735–2743CrossRefGoogle Scholar
  23. Heeger EF (1948) Oenothera biennis L., die Gemeine Nachtkerze, als Nutzpflanze. Pharmazie 3:273–275PubMedGoogle Scholar
  24. Hegi G (1927) Illustrierte Flora von Mittel-Europa, Bd V (2). Lehmanns, München, pp 857–873Google Scholar
  25. Hegnauer R (1969) Chemotaxonomie der Pflanzen, Bd 5. Birkhäuser, Basel, pp 222–226CrossRefGoogle Scholar
  26. Hegnauer R (1986) Chemotaxonomie der Pflanzen, Bd 7. Birkhäuser, Basel, pp 298–313Google Scholar
  27. Hojden B, Lamer-Zarawska E (1990) Badania uprawowe niektórych gatunków z rodzaju Oenothera L. Herba Pol 36:139–143Google Scholar
  28. Hopkins BJ, Scheinmann F (1971) Triterpenes in the seed oil of evening primrose, Oenothera lamarckiana. Phytochemistry 10:1956–1961CrossRefGoogle Scholar
  29. Horrobin DF (1990) Gamma linolenic acid: An intermediate in essential fatty acid metabolism with potential as an ethical pharmaceutical and as a food. In: Johnson S, Johnson FN (eds) Gamma linolenic acid. Rev Contemp Pharmacother 1:1–45Google Scholar
  30. Howard GZ, Mabry TJ (1970) Myricetin 3–0-methyl ether 3′-0-beta-D-glucoside, the major flavonoid of Oenothera speciosa (Onagraceae). Phytochemistry 9:2413–2414CrossRefGoogle Scholar
  31. Howard GZ, Mabry TJ, Raven PH (1972) Distribution of flavonoids in twenty-one species of Oenothera. Phytochemistry 11:289–291CrossRefGoogle Scholar
  32. Hudson BJF (1984) Evening primrose (Oenothera spp.) oil and seed. JAOCS 61:540–543CrossRefGoogle Scholar
  33. Ihrig M, Blume H (1994) Nachtkerzenöl Präparate: ein Qualitätsvergleich. Pharm Ztg 139:34–42Google Scholar
  34. Jankowski WJ, Stołyhwo A (1995) Unusual fatty acid composition of cuticular lipids from leaves of Oenothera. J Plant Physiol 145:215–220CrossRefGoogle Scholar
  35. Jean R, Linder R, Hoffmann F, Thomas E, Wenzel G (1976) Comportement in vitro de microspores d’Oenothera coranifera Renne. C R Acad Sci Paris 283:781–784Google Scholar
  36. Kadota S, Takamori Y, Kikuchi T, Motegi A, Ekimoto H (1990) Woodfruticosin, an inhibitor of DNA topoisomerase II from Woodfordia fruticosa Kurz. Tetrahedron Lett 31:393–396CrossRefGoogle Scholar
  37. Kagan J (1967) Myricetin 3-β-galactoside, the main flavonoid from Oenothera lavandulaefolia T. and G. Phytochemistry 6:317–318CrossRefGoogle Scholar
  38. Kakiuchi N, Hattori M, Namba T (1985) Inhibitory effect of tannins on reverse transcriptase from RNA tumor virus. J Nat Prod 48:614–621PubMedCrossRefGoogle Scholar
  39. Kleijnen J (1994) Evening primrose oil. Br Med J 309:824–825CrossRefGoogle Scholar
  40. Kowalewski Z, Kowalska M, Skrzypczakowa L (1968) Flavonols of Oenothera biennis L. Dissert Pharm Pharmacol 20:573–575Google Scholar
  41. Krzaczek T, Bogucka-Kocka A (1994) Chromatographical analysis of phenolic acids in some species of the genus Oenothera. Eur J Pharm Sci 2(1–2):124CrossRefGoogle Scholar
  42. Krzaczek T, Bogucka-Kocka A, Śnieżko R (1995) The phenolic acids of some species of the Oenothera L. genus. Acta Soc Bot Pol 64:41–44Google Scholar
  43. Krzyżaniak M, Segiet-Kujawa E (1991) Determination of γ-linolenic acid in Oenothera biennis L.. Herba Pol 37:57–62Google Scholar
  44. Lamer-Zarawska E, Hojden B (1989a) Polish Oenothera a source of essential unsaturated fatty acids (EFA). Int Symp Medicines of plant origin in modern therapy with special reference to Kampo Medicine, PragueGoogle Scholar
  45. Lamer-Zarawska E, Hojden B (1989b) Rośliny rodzaju Oenothera L. zródłem niezbęnych nienasyconych kwasów tłuszczowych. Herba Pol 35:151–156Google Scholar
  46. Lamer-Zarawska E, Hojden B, Szymczak J (1989) Badanie olejów z nasion niektórych gatunków Oenothera L. Herba Pol 35:165–170Google Scholar
  47. Lamer-Zarawska E, Hojden B, Nowak W (1990) Sposób agrotechniki prowadzacy do skrócenia okresu wegetacji 3 nowych roślin oleistych z rodzaju Oenothera L. — inventive project, AM WrocławGoogle Scholar
  48. Lamer-Zarawska E, Hojden B, Milan A (1993) Wpływ zanieczyszczeń środowiska na stopień skażenia wiesiołka (Oenothera L.) przez metale ciężkie. Herba Pol 39:217–222Google Scholar
  49. Levy A, Palevitch D, Ranen C (1993) Increasing gamma linolenic acid in evening primrose grown under hot temperatures by breeding early cultivars. Acta Hortic 330:219–223Google Scholar
  50. Madaus G (1938) Lehrbuch der Biologischen Heilmittel, Bd 3, Thieme, Leipzig, pp 2002–2005Google Scholar
  51. Madaus G (1938) Lehrbuch der Biologischen Heilmittel, Bd 3, Thieme, Leipzig, pp 2696Google Scholar
  52. Magnus V, Nigović B, Hangarter RP, Good NE (1992) N-(indol-3-ylacetyl) amino acids as sources of auxin in plant tissue culture. J Plant Growth Regul 11:19–28CrossRefGoogle Scholar
  53. Martinez LD, Noher de Halac I (1995) Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries. Plant Cell Tissue Organ Cult 42:91–96CrossRefGoogle Scholar
  54. Masquelier J, Delaunay D (1965) Bactericidal action of the phenolic acids of wine. Bull Soc Pharm Bord 104:152–160Google Scholar
  55. Middleton EJR, Kandaswami (1994) The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. In: Harborne JB (ed) The flavonoids: advances in research since 1986. Chapman and Hall, London, pp 619–652Google Scholar
  56. Miyamoto K, Kishi N, Koshiura R, Yoshida T, Hatano T, Okuda T (1987) Relationship between the structures and the antitumor activities of tannins. Chem Pharm Bull 35:814–822PubMedCrossRefGoogle Scholar
  57. Miyamoto K, Nomura M, Sasakura M, Matsui E, Koshiura R, Murayama T, Furukawa T, Hatano T, Yoshida T, Okuda T (1993a) Antitumor activity of oenothein B, a unique macrocyclic ellagitannin. Jpn J Cancer Res 84:99–103PubMedCrossRefGoogle Scholar
  58. Miyamoto K, Murayama T, Nomura M, Hatano T, Yoshida T, Furukawa T, Koshiura R, Okuda T (1993b) Antitumor activity and interleukin 1 induction by tannins. Anticancer Res 13:37–42PubMedGoogle Scholar
  59. Motoyama M, Isono Y, Mori I, Matsui E, Yokoi H, Miyamoto K, Koshiura R (1988) Antitumor activities of tannins (ellagitannin) in mice. Res Home Med 7:55–58 (in Japanese)Google Scholar
  60. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  61. Nagata T, Takebe (1971) Plating of isolated tobacco mesophyll protoplasts on agar medium. Planta 99:12–20CrossRefGoogle Scholar
  62. Nakashima H, Murakami T, Yamamoto N, Sakagami H, Tanuma S, Hatano T, Yoshida T, Okuda T (1992) Inhibition of human immunodeficiency viral replication by tannins and related compounds. Antiviral Res 18:91–103PubMedCrossRefGoogle Scholar
  63. Neumann G, Schwemmle B (1993) Flavonoids from Oenothera seedlings: identification and extranuclear control of their biosynthesis. J Plant Physiol 142:135–143CrossRefGoogle Scholar
  64. Neumann G, Schwemmle B (1994) Organ- and tissue-specific biosynthesis of flavonoids in seedlings of Oenothera odorata (Onagraceae). Bot Acta 107:95–102Google Scholar
  65. Nichols BW, Wood BJB (1968) The occurrence and biosynthesis of gamma-linolenic acid in a blue-green alga, Spirulina platensis. Lipids 3:46–50PubMedCrossRefGoogle Scholar
  66. Nishizawa M, Yamagishi T, Dutschman GE, Parker WB, Bodner AJ, Kilkuskie RE, Cheng Y-C, Lee K-H (1989) Anti-AIDS agents, 1. Isolation and characterization of four new tetragalloylquinic acids as a new class of HIV reverse transcriptase inhibitors from tannic acid. J Nat Prod 52:762–768PubMedCrossRefGoogle Scholar
  67. Nonaka G-I, Nishioka I, Nishizawa M, Yamagishi T, Kashiwada Y, Dutschman GE, Bodner AJ, Kilkuskie RE, Cheng Y-C, Lee K-H (1990) Anti-AIDS agents, 2: Inhibitory effects of tannins on HIV reverse transcriptase and HIV replication in H9 lymphocyte cells. J Nat Prod 53:587–595PubMedCrossRefGoogle Scholar
  68. Okuda T, Hatano T, Ogawa N, Kira R, Matsuda M (1984) Cornusin A, a dimeric ellagitannin forming four tautomers, and accompanying new tannins in Cornus officinalis. Chem Pharm Bull 32:4662–4665CrossRefGoogle Scholar
  69. Okuda T, Yoshida T, Hatano T (1989) Ellagitannins as active constituents of medicinal plants. Planta Med 55:117–122PubMedCrossRefGoogle Scholar
  70. Okuda T, Yoshida T, Hatano T (1993) Classification of oligomeric hydrolysable tannins and specificity of their occurrence in plants. Phytochemistry 32:507–521CrossRefGoogle Scholar
  71. Osamu Y, Tadashi F (1987) γ-Linolenic acid manufacture by Oenothera tissue culture. Jpn Kokai Tokkyo Koho JP: 62, 210, 995 (87, 210, 995); CA 1988, 108:73837Google Scholar
  72. Penso G (1983) Index plantarum medicinalium totius mundi corumque synonymorum. OEMF, Milano, pp 677–678Google Scholar
  73. Ratledge C (1991) Microorganisms for lipids. Acta Biotechnol 11:429–438CrossRefGoogle Scholar
  74. Raven PH In: Tutin TG et al. (eds) Flora Europaea, vol 2. University Press, Cambridge, pp 306–308Google Scholar
  75. Raven PH, Dietrich W, Stubbe W (1979) An outline of the systematics of Oenothera subsect Euoenothera (Onagraceae). Syst Bot 4:242–252CrossRefGoogle Scholar
  76. Renner O (1956) Europäische Wildarten von Oenothera. Planta 47:219–254CrossRefGoogle Scholar
  77. Rickett HW (1970) In: Steere WC (ed) Wild flowers of the United States. McGraw-Hill, New York pp 318–329Google Scholar
  78. Rickett HW (1971) In: Steere WC (ed) The evening-primrose family (Onagraceae). McGraw-Hill, New York, pp 294–298Google Scholar
  79. Rostański K (1965) Kilka nowych taksonów rodzaju Oenothera L.z podrodzaju Oenothera. Fragm Flor Geobot 11:499–523Google Scholar
  80. Rostanski K (1985) Zur Gliederung der Subsektion Oenothera (Sektion Oenothera, Oenothera L. Onagraceae). Feddes Reperì 96:3–14Google Scholar
  81. Rostański K, Wika S (1988) Materiały do rozmieszczenia gatunków rodzaju Oenothera L. wśrodkowej części Wyżyny Krakowsko-Wieluńskiej. Frag Flor Geobot 33:33–40Google Scholar
  82. Rostański K (1992) Some taxonomic problems within the genus Oenothera L. and the species differentiation of the Oenthera in Poland. In: Oil from Oenothera in prophylaxis and therapy. SympŁódź, Book of Abstracts, pp 2–8 (in Polish)Google Scholar
  83. Sewón P, Tyystjärvi E (1993) Stearidonic and γ-linolenic acid contents of common borage leaves. Phytochemistry 33:1029–1032CrossRefGoogle Scholar
  84. Shimizu S, Shinmen Y, Kawashima H, Akimoto K, Yamada H (1988) Fungal mycelia as a novel source of eicosapentaenoic acid. Activation of enzymes involved in eicosapentaenoic acid. Biochem Biophys Res Commun 150:335–341PubMedCrossRefGoogle Scholar
  85. Shishkin BK, Bobrow EG (1949) Flora USSR, vol 15, Academy of Sciences USSR. Moscow, pp 628–630Google Scholar
  86. Skrzypczak L, Wesołowska M, Thiem B (1988) Callus culture and micropropagation of Eustoma grandiflorum Shinn. Acta Soc Bot Pol 57:423–430Google Scholar
  87. Skrzypczak L, Skrzypczak-Pietraszek E, Lamer-Zarawska E, Hojden B (1994) Micropropagation of Oenothera biennis L. and an assay of fatty acids. Acta Soc Bot Pol 63:173–177Google Scholar
  88. Stubbe W, Herrmann RG (1982) Selection and maintenance of plastome mutants and interspecific genome/plastome hybrids from Oenothera. In: Edelman M, Hallick RB, Chua N-H (eds) Methods in chloroplast molecular biology, Elsevier Biomedical Press, Amsterdam, pp 149–165Google Scholar
  89. Su J-D, Osawa T, Kawakishi S, Namiki M (1988) Tannin antioxidants from Osbeckia chinensis. Phytochemistry 27:1315–1319CrossRefGoogle Scholar
  90. Suzuki S, Fujino H, Yamazaki N, Tatsuo Y, Yoshizaki M (1990) Propagation of Oenothera erythrosepala Borbas by shoot apex culture. Jpn J Breed 40:367–370Google Scholar
  91. Szafer W, Pawłowski B (eds) (1959) Flora Polska, vol 8 PWN, Warszawa, pp 211–219Google Scholar
  92. Szepczyńska K, Wolbiś M (1962) Fenolokwasy i flawonoidy Oenothera biennis L. 15th Naukowy Zjazd Polskiego Towarzystwa Farmaceutycznego, Postępy Nauk Farmaceutycznych, Warszawa p 220 (in Polish)Google Scholar
  93. Symposium (1992, 1995) Oil from Oenothera in prophylaxis and therapy. Łódż October 9–10; September 5–7, Books of Abstracts (in Polish)Google Scholar
  94. Takeo M, Michiko K, Yoshinori M (1987) γ-Linolenic acid ester containing oil and lipid manufacture by Oenothera tissue culture. Jjn Kokai Tokkyo Koho. JP: 62,195, 091 (87,195, 229); CA 1988,108:110878Google Scholar
  95. Thiem B, Skrzypczak-Pietraszek E, Skrzypczak L (1994) In vitro culture of some Oenothera species. 8th Int Congr Plant tissue and cell culture, Firenze, Abstracts, 176ppGoogle Scholar
  96. Thiem B, Skrzypczak L, Lamer-Zarawska E (1995) Mikrorozmn<inline>anie gatunków rodzaju Oenothera L. II Symp Oil from Oenothera in prophylaxis and therapy, Łódż, Book of Abstracts, pp 71–74 (in Polish)Google Scholar
  97. Thiem B, Skrzypczak L, Lamer-Zarawska E (1998) Fatty acids and phenolic compounds in Oenothera species from in vitro culture. Acta Soc Bot Pol (in press)Google Scholar
  98. Traitler H, Winter H, Richli U, Ingenbleek Y (1984) Characterization of γ-linolenic acid in Ribes seed. Lipids 19:923–928PubMedCrossRefGoogle Scholar
  99. Wagner H (1985) New plant phenolics of pharmaceutical interest. Annu Proc Phytochem Soc Eur 24:409–425Google Scholar
  100. Wagner WL, Stockhouse RE, Klein WM (1985) The systematics and evolution of the Oenothera caespitosa species complex (Onagraceae). Monogr Syst Bot vol 12, Missouri Botanical Garden, pp 1–103CrossRefGoogle Scholar
  101. Wolf RB, Kleiman R, England RE (1983) New sources of γ-linolenic acid. JAOCS 60:1858–1860CrossRefGoogle Scholar
  102. Wolfson R, Sears BB (1989) A reduction in the abundance of calcium oxalate crystals in Oenothera improves the yield of intact chloroplasts. Plant Cell Environ 12:109–112CrossRefGoogle Scholar
  103. Yaniv Z, Ranen C, Levy A, Palevitch D (1989) Effect of temperature on the fatty acid composition and yield of evening primrose (Oenothera lamarckiana) seeds. J Exp Bot 40:609–613CrossRefGoogle Scholar
  104. Yoshida T, Chou T, Matsuda M, Yasuhara T, Yazaki K, Hatano T, Nitta A, Okuda T (1991) Woodfordin D and Oenothein A, trimeric hydrolyzable tannins of macro-ring structure with antitumor activity. Chem Pharm Bull 39:1157–1162PubMedCrossRefGoogle Scholar
  105. Yoshida T, Chou T, Shingu T, Okuda T (1995) Oenotheins D, F and G, hydrolysable tannin dimers from Oenothera laciniata. Phytochemistry 40:555–561CrossRefGoogle Scholar
  106. Zinsmeister HD, Bartl S (1971) The phenolic compounds of Oenothera. Phytochemistry 10:3129–3132CrossRefGoogle Scholar
  107. Zinsmeister HD, Biering W (1973) Onagraceae, flavonol-glycosides in Oenothera hookeri. Phytochemistry 12:234CrossRefGoogle Scholar
  108. Zinsmeister HD, Schels H (1975) Onagraceae, flavonol-glycosides in Oenothera berteriana. Tabulated Phytochemical Reports. Phytochemistry 14:1468Google Scholar
  109. Zinsmeister HD, Kauer H, Rossmann G (1965) Über die Gerbstoffe von Oenothera hookeri. Planta 66:301–309CrossRefGoogle Scholar
  110. Zinsmeister HD, Hopf H, Kauer H, Bartl S (1970) Gerbstoff — Untersuchungen bei der Gattung Oenothera L. Planta Med 18:160–167PubMedCrossRefGoogle Scholar
  111. Zinsmeister HD, Plitzko I, Schels H (1977) Flavonol glycosides in South American species of Oenothera Sect Oenothera. Phytochemistry 16:497CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  1. 1.Department of Pharmaceutical BotanyK. Marcinkowski University of Medical Sciences in PoznańPoznańPoland

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