Immune Stimulation by Oligonucleotides

  • A. M. Krieg
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 131)


As has been pointed out previously ([Stein and Cheng 1993]), DNA is a polyanion. Like other polyanions, DNA can have a broad range of biologic effects. Native or modified DNA backbones can cause immune activation which is concentration-and length-dependent and sequence-independent. However, the different backbones currently under development for antisense applications differ greatly in the degree to which they induce immune effects. As might be expected, neutral backbones have minimal, if any, immune effects (A.M. Krieg, unpublished observation). Among the charged backbones that have been carefully investigated, the greatest magnitude of nonspecific effects are seen with phosphorothioate oligodeoxynucleotides (PS-ODNs) ([Stein and Cheng 1993]).


Immune Stimulation Immune Effect Severe Combine Immunodeficiency Disease Phosphorothioate Oligodeoxynucleotides Natural Killer Lytic Activity 
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© Springer-Verlag Berlin Heidelberg 1998

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  • A. M. Krieg

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