Low temperature fermentation of wine and beer by cold-adapted and immobilized yeast cells

  • M. Kanellaki
  • A. A. Koutinas

Abstract

Over the last 20 years considerable research and development have been made in wine making and brewing technology with the aim of improving the productivity and quality, taste and aroma of wine and beer. Use of selected cultures and genetically modified yeasts with desired traits, use of immobilized cells on several supports, enzymatic treatments, addition of adjuncts to malt, modern development in fermentor design and low-temperature fermentation are some technological innovations in alcoholic fermentation. Among these technological innovations, lowtemperature fermentation by cold-adapted and immobilized yeast cells is reviewed here.

Keywords

Sugar Cellulose Glycerol Sludge Aeration 

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References

  1. 1.
    Ough CS, Amerine MA. Controlled fermentation. VI. Effects of temperature and handling on rates, composition, and quality of wines. Am J Enol Viticult 1961; 12:117–128.Google Scholar
  2. 2.
    Ough CS. Fermentation rates of grape juice. I. Effects of temperature and composition on white juice fermentation rates. Am J Enol Viticult 1964; 15:167–177.Google Scholar
  3. 3.
    Nagodawithana TW, Castellano C, Steinkraus KH. Effect of dissolved oxygen, temperature, initial cell count and sugar concentration on the viability of Saccharomyces cerevisiae in rapid fermentation. Appl Microbiol 1974; 28:383–391.Google Scholar
  4. 4.
    Van Uden N, Da Cruz Duarte H. Effects of ethanol on the temperature profile of Saccharomyces cerevisiae. Z Allg Mikrobiol 1981; 21:743–750.CrossRefGoogle Scholar
  5. 5.
    Laluce C, Palmieri MC, Lopes da Cruz RC. Growth and fermentation characteristics of new selected strains of Saccharomyces at high temperatures and high cell densities. Biotechnol Bioeng 1991; 37:528–536.CrossRefGoogle Scholar
  6. 6.
    Hacking AJ, Taylor IWF, Hanas CM. Selection of yeast able to produce ethanol from glucose at 40°C. Appl Microbiol Biotechnol 1984; 19:361–363.CrossRefGoogle Scholar
  7. 7.
    Perego L Jr, Cabral de S Dias JM, Koshimizu LH, De Melo Cruz MR, Borzani W, Vairo MLR. Influence of temperature, dilution rate and sugar concentration on the establishment of steady-state in continuous ethanol fermentation of molasses. Biomass 1985; 6:247–256.CrossRefGoogle Scholar
  8. 8.
    Caro I, Perez L, Cantero D. Development of a kinetic model for the alcoholic fermentation of must. Biotechnol Bioeng 1991; 38:742–748.CrossRefGoogle Scholar
  9. 9.
    Ough CS. Fermentation rates of grape juice. II. Effects of initial °Brix, pH, and fermentation temperature. Am J Enol Viticult 1966; 17:20–26.Google Scholar
  10. 10.
    Bertrand G, Silberstein L. Does fermentation of sugar normally produce methanol? Compt Rend 1950; 230:800–803.Google Scholar
  11. 11.
    Lee CH, Robinson WB, Van Buren JP, Acree TE, Stoewsand GS. Methanol in wines in relation to processing and variety. Am J Enol Viticult 1975; 26:184–187.Google Scholar
  12. 12.
    Stella C, Testa F, Viviani C, Sabatelli MP. Heat treatment of grapes: effect on wines from different varieties. Vignevini 1991; 18:47–50.Google Scholar
  13. 13.
    Gardner N, Rodrigue N, Champagne CP. Combined effects of sulfites, temperature, and agitation time on production of glycerol in grape juice by Saccharomyces cerevisiae. Appl Environ Microbiol 1993; 59:2022–2028.Google Scholar
  14. 14.
    Ough CS, Fong D, Amerine MA. Glycerol in wine: determination and some factors affecting formation. Am J Enol Viticult 1972; 23:1–5.Google Scholar
  15. 15.
    Rankine BC, Bridson DA. Glycerol in Australian wines and factors influencing its formation. Am J Enol Viticult 1971; 22:6–12.Google Scholar
  16. 16.
    Castellari L, Magrini A, Passarelli P, Zambonelli C. Effect of must fermentation temperature on minor products formed by cryotolerant and non-cryotolerant Saccharomyces cerevisiae strains. Ital J Food Sci 1995; 7:125–132.Google Scholar
  17. 17.
    Giudici P, Zambonelli C, Passarelli P, Castellari L. Improvement of wine composition with cryotolerant Saccharomyces strains. Am J Enol Vitic 1995; 46:143–147.Google Scholar
  18. 18.
    Ough CS, Guymon JF, Crowell EA. Formation of higher alcohols during grape juice fermentations at various temperatures. J Food Sci 1966; 31:620–625.CrossRefGoogle Scholar
  19. 19.
    Stewart GG, Russell I. Centenary review. One hundred years of yeast research and development in the brewing industry. J Inst Brew 1986; 92:537–558.Google Scholar
  20. 20.
    Ruzic N. Effect of temperature on yeast activity and chemical composition of wine. Zbomik Radova 1991; 22:35–44.Google Scholar
  21. 21.
    Bertolini L, Zambonelli C, Giudici P, Castellari L. Higher alcohols production by cryotolerant Saccharomyces strains. Am J Enol Viticult 1996; 47:343–345.Google Scholar
  22. 22.
    Bakoyianis V, Kana K, Kaliafas A, Koutinas AA. Low temperature wine making by kissirissupported biocatalyst: Volatile by-products. J Agric Food Chem 1993; 41:465–468.CrossRefGoogle Scholar
  23. 23.
    Bakoyianis V. Formation of volatile by-products in the continuous wine making by immobilized cells on mineral kissiris, y-alumina and calcium alginates and an application in industrial scale pilot plant of a fermentation in the presence of kissiris. PhD Thesis, University of Patras, Greece, 1995:140–150.Google Scholar
  24. 24.
    Killian E, Ough CS. Fermentation esters - formation and retention as affected by fermentation temperature. Am J Enol Viticult 1979; 30:301–305.Google Scholar
  25. 25.
    Gomez E, Laencina J, Martinez A. Vinification effects on changes in volatile compounds in wine. J Food Sci 1994; 59:406–409.CrossRefGoogle Scholar
  26. 26.
    Martini A. Origin and domestication of the wine yeast Saccharomyces cerevisiae. J Wine Res 1993; 4:165–176.CrossRefGoogle Scholar
  27. 27.
    Polsinelli M, Romano P, Suzzi G, Mortimer R. Multiple strains of Saccharomyces cerevisiae on a single vine. Lett Appl Microbiol 1996; 23:110–114.CrossRefGoogle Scholar
  28. 28.
    Versavaund A, Courcoux P, Roulland C, Dulau L, Hallet J-N. Genetic diversity and geographical distribution of wild Saccharomyces cerevisiae strains from the wine-producing area of Charentes, France. Appl Environ Microbiol 1995; 61:3521–3529.Google Scholar
  29. 29.
    Brown CM, Campbell I, Priest FG. Introduction to Biotechnology. Oxford: Blackwell Scientific Publications, 1987:93.Google Scholar
  30. 30.
    Heard GM, Fleet GH. Growth of natural yeast flora during the fermentation of inoculated wines. Appl Environ Microbiol 1985; 50:727–728.Google Scholar
  31. 31.
    Gao C, Fleet. GH. The effects of temperature and pH on the ethanol tolerance of the wine yeasts, Saccharomyces cerevisiae, Candida stellata and Kloeckera apiculata. J Appl Bacteriol 1988; 65:405–409.CrossRefGoogle Scholar
  32. 32.
    Kunkee RE. Selection and modification of yeasts and lactic acid bacteria for wine fermentation. Food Microbiol 1984; 1:315–332.CrossRefGoogle Scholar
  33. 33.
    Heard GM, Fleet GH. The effects of temperature and pH on the growth of yeast species during the fermentation of grape juice. J Appl Bacteriol 1988; 65:23–28..CrossRefGoogle Scholar
  34. 34.
    Koutinas AA, Pefanis ST. Biotechnology of Foods and Drinks. University of Patras, Greece, 1992:149–153.Google Scholar
  35. 35.
    Argiriou T, Kalliafas A, Psarianos K, Kana K, Kanellaki M, Koutinas AA. New alcohol resistant strains of Saccharomyces cerevisiae species for potable alcohol production using molases. Appl Biochem Biotechnol 1992; 36:153–161.CrossRefGoogle Scholar
  36. 36.
    Argiriou T, Kaliafas A, Psarianos K, Kanellaki M, Voliotis S, Koutinas AA. Psychrotolerant Saccharomyces cerevisiae strains after an adaptation treatment for low temperature wine making. Process Biochem 1996; 31:639–643.CrossRefGoogle Scholar
  37. 37.
    Miklos I, Sipiczki M, Benko Z. Osmotolerant yeasts isolated from Tokaj wines. J Basic Microbiol 1994; 34:379–385.CrossRefGoogle Scholar
  38. 38.
    Kishimoto M, Goto S. Growth temperatures and electrophoretic karyotyping as tools for practical discrimination of Saccharomyces bayanus and Saccharomyces cerevisiae. J Gen Appl Microbiol 1995; 41:239–247.CrossRefGoogle Scholar
  39. 39.
    Castellari L, Pacchioli G, Zambonelli C, Tini V, Grazia L. Isolation and initial characterization of cryotolerant Saccharomyces strains. Ital J Food Sci 1992; 4:179–186.Google Scholar
  40. 40.
    Hantula J, Kurki A, Vuoriranta P, Bamford DH. Rapid classification of bacterial strains by SDS-polyacrylamide gel electrophoresis: population dynamics of the dominant dispersed phase bacteria of activated sludge. Appl Microbiol Biotechnol 1991; 34:551–555.CrossRefGoogle Scholar
  41. 41.
    Nikolova P, Ward OP. Production of L-phenyl-acetyl carbinol by biotransformation product and by-product formation and activities of the key enzymes in wild-type and ADH isoenzyme mutants of Saccharomyces cerevisiae. Biotechnol Bioeng 1991; 38:493–498.CrossRefGoogle Scholar
  42. 42.
    Giolfi G. Acetaldehyde formation in relation to yeast nitrogen nutrition during alcoholic fermentation. Riv Vitic Enol 1983; 36:431.Google Scholar
  43. 43.
    Ferreira da Silva L, Kamiya NF, Oliveira MS, Alterthum E Comparison of preservation methods applied to yeasts used for ethanol production in Brazil. Rev Microbiol Sao Paulo 1992; 23:177–182.Google Scholar
  44. 44.
    Campbell I. Culture, storage, isolation and identification of yeasts. In: Campbell I, Duffus JH, eds. Yeast: A Practical Approach, 2nd ed. Oxford: IRLPress, 1991:1–2.Google Scholar
  45. 45.
    Berny JF, Hennebert GL. Viability and stability of yeast cells and filamentous fungus spores during freeze-drying. Effects of protectants and cooling rates. Mycologia 1991; 83:805–815.CrossRefGoogle Scholar
  46. 46.
    Hough JS. The Biotechnology of Malting and Brewing, 1st paperback ed. Cambridge: Cambridge University Press, 1991:102.Google Scholar
  47. 47.
    Loureiro V. Portuguese contribution on immobilized yeast cell, for sparkling wine production. In: Colagrande O, ed. Sviluppi della Biotecnologia nella Produzione dello Spumante Classico. Atti del 4° Simposio Internationale sul Vino, Pavia. Pinerolo Italy: Chiriotti Publishers, 1990:74–77.Google Scholar
  48. 48.
    Smith D. Tolerance to freezing and thawing. In: Jennings DH, ed. Stress Tolerance of Fungi, 1st ed. New York: Marcel Dekker, Inc, 1993:146, 151.Google Scholar
  49. 49.
    Argiriou T, Kanellaki M, Voliotis S, Koutinas AA. Kissiris-supported yeast cells: High biocatalytic stability and productivity improvement by successive preservations at 0°C. J Agric Food Chem 1996; 44:4028–4031.CrossRefGoogle Scholar
  50. 50.
    Atlas RM, Bartha R. Effects of abiotic factors and environmental extremes on microorganisms. In: Brady EB, senior ed. Microbial Ecology. Fundamentals and Applications, 3rd ed. USA: The Benjamin/Cummings Publishing Company. 1993:215.Google Scholar
  51. 51.
    Russell PD. Fermenter and bio-reactor design. In: King RD, Cheetham PSJ, eds. Food Biotechnology 1. London New York: Elsevier Applied Science, 1987:6–8.Google Scholar
  52. 52.
    Mauricio JC, Moreno J, Medina M, Ortega J. Fermentation of Pedro Ximenez musts at various temperatures and different degrees of ripeness. Belg J Food Chem Biotechnol 1986; 41:71–76.Google Scholar
  53. 53.
    Kusewicz D. Characteristics of fermentation abilities in some varieties of wine yeast of cryophilic types. Acta Aliment Pol 1975; 1:235–246.Google Scholar
  54. 54.
    Kishimoto M, Shinohara T, Soma E, Goto S. Selection and fermentation properties of cryophilic wine yeasts. J Ferment Bioeng 1993; 75:451–453.CrossRefGoogle Scholar
  55. 55.
    Bakoyianis V, Kanellaki M, Kaliafas A, Koutinas AA. Low temperature wine making by immobilized cells on mineral kissiris. J Agric Food Chem 1992; 40:1293–1296.CrossRefGoogle Scholar
  56. 56.
    Bardi EP, Koutinas AA. Immobilization of yeast on defignified cellulosic material for room temperature and low temperature wine making. J Agric Food Chem 1994; 42:221–226.CrossRefGoogle Scholar
  57. 57.
    Bardi EP, Bakoyianis V, Koutinas AA, Kanellaki M. Room temperature and low temperature wine making using yeast immobilized on gluten pellets. Process Biochem 1996; 31:425–430.CrossRefGoogle Scholar
  58. 58.
    Jackson RS. Wine Science. Principles and Applications, 1st ed. London: Academic Press, 1994:245.Google Scholar
  59. 59.
    Kishimoto M. Fermentation characteristics of hybrids between the cryophilic wine yeast Saccharomyces bayanus and the mesophilic wine yeast Saccharomyces cerevisiae. J Ferment Bioeng 1994; 77:432–435.CrossRefGoogle Scholar
  60. 60.
    Hara S, Iimura Y, Oyama H, Kozeki T, Kitano K, Otsuka KI. The breeding of cryophilic killer wine yeasts. Agric Biol Chem 1981; 45:1327–1334.CrossRefGoogle Scholar
  61. 61.
    Eustace R, Thornton RJ. Selective hybridization of wine yeasts for higher yields of glycerol. Can J Microbiol 1987; 33:112–117.CrossRefGoogle Scholar
  62. 62.
    Thornton RJ. Selective hybridization of pure culture wine yeasts. II. Improvement of fermentation efficiency and inheritance of SO2 tolerance. Eur J Appl Microbiol Biotechnol. 1982; 14:159–164.CrossRefGoogle Scholar
  63. 63.
    Watari J, Takata Y, Ogawa M, Nishikawa N, Kaminura M. Molecular cloning of a flocculation gene in Saccharomyces cerevisiae. Plasmid DNA purification from E. coli culture. Agr Biol Chem 1989; 53:901–903.CrossRefGoogle Scholar
  64. 64.
    Guerzoni ME, Marchetti R, Giudici P. Modifications of aroma components of wines obtained by fermentation with Saccharomyces cerevisiae mutants. Bull OW 1985; 58:228–234.Google Scholar
  65. 65.
    Lee S, Villa K, Patino H. Yeast strain development for enhanced production of desirable alcohols/esters in beer. J Amer Soc Brew Chem 1995;53:153–156.Google Scholar
  66. 66.
    Navarro JM, Durant G. Modification of yeast metabolism by immobilization onto porous glass. Eur J Appl Microbiol 1977; 4: 243–254.CrossRefGoogle Scholar
  67. 67.
    Doran PM, Bailey JE. Effects of immobilization on growth, fermentation properties and macromolecular composition of Saccharomyces cerevisiae attached to gelatin. Biotechnol Bioeng 1986; 28:73–87.CrossRefGoogle Scholar
  68. 68.
    Gallazo JL, Bailey JE. Fermentation pathway kinetics and metabolic flux control in suspended and immobilized Saccharomyces cerevisiae. Enzyme Microbiol Technol 1990; 12:162–172.CrossRefGoogle Scholar
  69. 69.
    Hilge-Rotmann B, Rehm HJ. Comparison of fermentation properties and specific enzyme activities of free and calcium-alginate-entrapped Saccharomyces cerevisiae. Appl Microbiol Biotechnol 1990; 33:54–58.CrossRefGoogle Scholar
  70. 70.
    Shimobayashi Y, Tominaga K. Application of biotechnology in the food industry. I. Brewing of white wine by a bioreactor. Hokaidoritsu Kogyo Shikenjo Hokoku 1986; 285:199–204.Google Scholar
  71. 71.
    Nakanishi K, Yokotsuka K. Fermentation of white wine from Koshu grape using immobilized yeast. Nippon Shokuhin Kogyo Gakkaishi 1987; 34:362–369.CrossRefGoogle Scholar
  72. 72.
    Mori S. Fruit wine or sake manufacture by bioreactor. Jpn Kokai Tokkyo Koho JP 6261, 577; 18 March.1987.Google Scholar
  73. 73.
    Fumi M, Trioli G, Colagrande O. Preliminary assessment on the use of immobilized yeast cells in sodium alginate for sparkling wine processes. Biotechnol Lett 1987; 9:339–342.CrossRefGoogle Scholar
  74. 74.
    Hamdy MK. Method for rapidly fermenting alcoholic beverages. Patent Cooperation Treaty Int Appl WO 9005, 189; 17 May 1990.Google Scholar
  75. 75.
    Ageeva NM, Merzhanian AA, Sobolev EM. Effect of yeast adsorption on the functional activity of the yeast cells and composition of wine. Mikrobiologiya 1985; 54:830–834.Google Scholar
  76. 76.
    Otsuka K. Wine making. Jpn Kokai Tokkyo Koho 80, 159, 789; 12 December 1980.Google Scholar
  77. 77.
    Lommi H, Advenainen J. Method using immobilized yeast to produce ethanol and alcoholic beverages. Eur Pat Appl EP 361, 165; 4 April 1990.Google Scholar
  78. 78.
    Kolpakchi AP, Isaeva VS, Zhvirblyanskaya A Yu, Kazantsev EN, Serova EN, Rattel NN. Fixation of brewer’s yeast to polymer materials. Prikl. Biokhim. Microbiol. 1976; 12:866–870.Google Scholar
  79. 79.
    Kolpakchi AP, Isaeva VS, Kazantsev EN, Fertman GI. Improvement in the fermentation of brewing wort with yeast fixed on a support. Ferment Spirt Prom - st 1980; 2:9–14.Google Scholar
  80. 80.
    Pardonova B, Polednikova M, Sedova H, Kahler M, Ludvik J. Biocatalyst for beer production. Brauwissenschaft 1982; 35:254–258.Google Scholar
  81. 81.
    Shindo S, Kamimura M. Immobilization of yeast with hollow PVA gel beads. J Ferment Bioeng 1990; 70:232–234.CrossRefGoogle Scholar
  82. 82.
    Moll M. Continuous brewing of beer. US Patent No 4,009,286, 1977.Google Scholar
  83. 83.
    Moll M, Duteutre B. Continuous beer preparation using adsorbed yeasts. Cell Immobilisees Colloque, Paris, France, 1979:173–185.Google Scholar
  84. 84.
    Godtfredsen SE, Ottesen M, Svensson B. Application of immobilized yeast and yeast comobilized with amyloglucosidase in the brewing process. Proc Congr Eur Brew Cony, Copenhagen Denmark 1981; 18:505–511.Google Scholar
  85. 85.
    Linko YY, Linko P. Continuous ethanol production by immobilized yeast reactor. Biotechnol Lett 1981; 3:21–26.CrossRefGoogle Scholar
  86. 86.
    Onaka T, Nakanishi K, Inoue T, Kubo S. Beer brewing with immobilized yeast. Bio/Technology 1985; 3:467–470.CrossRefGoogle Scholar
  87. 87.
    Nakanishi K, Murayama H, Sato H, Nagara A, Yasui T, Mitsui S. Continuous beer brewing with yeast immobilized on granular ceramic. Hakko Kogaku Kaishi 1989; 67:509–514.Google Scholar
  88. 88.
    Bardi EP, Koutinas AA, Soupioni MJ, Kanellaki ME. Immobilization of yeast on delignified cellulosic material for low temperature brewing. J Agric Food Chem 1996; 44:463–467.CrossRefGoogle Scholar
  89. 89.
    Bardi E, Koutinas AA, Kanellaki M. Room and low temperature brewing with yeast immobilized on gluten pellets. Process Biochem 1997; 32:691–696.CrossRefGoogle Scholar
  90. 90.
    Kana K, Kanellaki M, Psarianos C, Koutinas AA. Ethanol production by Saccharomyces cere-visiae immobilized on mineral kissiris. J Ferment Bioeng 1989; 68:144–147.CrossRefGoogle Scholar
  91. 91.
    Koutinas AA, Gourdoupis C, Psarianos C, Kaliafas A, Kanellaki M. Continuous potable alcohol production by immobilized Saccharomyces cerevisiae on mineral kissiris. Appl Biochem Biotechnol 1991; 30:203–216.CrossRefGoogle Scholar
  92. 92.
    Koutinas AA, Kanellaki M. Continuous potable alcohol production by Zymomonas mobilis on y-alumina pellets. J Food Sci 1990; 55:525–527,531.CrossRefGoogle Scholar
  93. 93.
    Iconomou L, Kanellaki M, Voliotis S, Agelopoulos K, Koutinas AA. Continuous wine making by delignified cellulosic materials supported biocatalyst. Appl Biochem Biotechnol 1996; 60:303–313.CrossRefGoogle Scholar
  94. 94.
    Bardi EP. Wine making and brewing by immobilized cells on delignified cellulosic material and gluten pellets. PhD Thesis, University of Patras, Greece, 1997.Google Scholar
  95. 95.
    Bakoyianis V, Koutinas AA, Agelopoulos K, Kanellaki M. Comparative study of kissiris, y-alumina and Ca-alginate as supports of cells for batch and continuous wine making at low temperatures. J Agric Food Chem 1997; 45:4884–4888.CrossRefGoogle Scholar
  96. 96.
    Bardi E, Koutinas AA, Psarianos C, Kanellaki M. Volatile by-products formed in low-temperature wine -making using immobilized yeast cells. Process Biochem 1997; 32:579–584.CrossRefGoogle Scholar
  97. 97.
    Suomalanien H, Lehtonen M. The production of aroma compounds by yeast. J Inst Brew 1979; 85:149–156.Google Scholar
  98. 98.
    Nykanen L. Formation and occurrence of flavor compounds in wine and distilled alcoholic beverages. Amer J Enol Viticult 1986; 37:84–96.Google Scholar
  99. 99.
    Rapp A, Mandery H. Wine aroma. Experentia 1986; 42:873–884.Google Scholar
  100. 100.
    Stashenko H, Macku C, Takayuki S. Monitoring volatile chemicals formed from must during yeast fermentation. J Agric Food Chem 1992; 40:2257–2259.CrossRefGoogle Scholar
  101. 101.
    Shinohara T, Saito K, Yanagida F, Goto S. Selection and hybridization of wine yeasts for improved winemaking properties: Fermentation rate and aroma productivity. J Ferment Bioeng 1994; 77:428–431.CrossRefGoogle Scholar
  102. 102.
    Wagener WWD, Wagener GWW. The influence of esters and fusel alcohols content upon the quality of dry white wines. S Mr J Agric Sci 1968; 11:469–476.Google Scholar
  103. 103.
    Soles RM, Ough CS, Kunkee RE. Ester concentration differences in wine fermented by various species and strains of yeasts. Am J Enol Viticult 1982; 33:94–98.Google Scholar
  104. 104.
    Holloway P, Subden RE. Volatile metabolites produced in a Riesling must by wild yeast isolates. Can Inst Sci Technol J 1991; 24:57–59.Google Scholar
  105. 105.
    Cabezudo MD, Gorostiza EF, Herraiz M, Fernandez-Biarange J, Garcia-Dominguez JA, Mol-era MJ. Mixed columns made to order in gas chromatography. IV. Isothermal selective separation of alcoholic and acetic fermentation products. J Chromat Sci 1978; 16:61–67.Google Scholar
  106. 106.
    Lee CY, Acree TE, Butts RM. Determination of methanol in wine by gas chromatography. Anal Chem 1975; 47:747–748.CrossRefGoogle Scholar
  107. 107.
    Gallazzo JL, Bailey JE. In vivo nuclear magnetic resonance analysis of immobilization effects on glucose metabolism of yeast Saccharomyces cerevisiae. Biotechnol Bioeng 1989; 33:1283–1289.CrossRefGoogle Scholar
  108. 108.
    Hough JS, Briggs DE, Stevens R, Young TW. Malting and Brewing Science, vol. 2. New York: Chapman and Hall Ltd Eds. 1982, reprinted 1987:692.Google Scholar
  109. 109.
    Pajunen E, Gronqvist A, Lommi H Continuous secondary fermentation and maturation of beer in an immobilized yeast reactor. MBAA Technical Quart 1989; 26:147–151.Google Scholar
  110. 110.
    Yamauchi Y, Okamoto T, Murayama H, Kajino K, Nagara A, Noguchi K. Rapid maturation of beer using an immobilized yeast bioreactor. 2. Balance of total diacetyl reduction and regeneration. J Biotechnol 1995; 38:109–116.CrossRefGoogle Scholar
  111. 111.
    Russell I, Stewart GG. Contribution of yeast and immobilization technology to flavor development in fermented beverages. Food Technol 1992; 46:146–150.Google Scholar
  112. 112.
    Norton S, D’Amore T. Physiological effects of yeast cell immobilization: Application for brewing. Enzyme Microbiol Technol 1994; 16:365–375.CrossRefGoogle Scholar
  113. 113.
    Bardi EP, Soupioni M, Koutinas AA, Kanellaki M. Effect of temperature on the formation of volatile by-products in brewing by immobilized cells. Food Biotechnol 1996; 10:203–217.CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • M. Kanellaki
    • 1
  • A. A. Koutinas
    • 1
  1. 1.Department of Chemistry, Section of Analytical, Environmental and Applied ChemistryUniversity of PatrasPatrasGreece

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