Abstract
The first identified protein kinase exhibiting direct activation by a Ras-related GTPase was a brain p21(Cdc42/Rac) activated kinase designated p65PAK(Manser et al 1994).The kinase(s) were originally identified in [γ32P]GTPCdc42 and [γ32P]GTP-Rac1 overlays of SDS-polyacrylamide gel fractionated brain proteins(Manser et al. 1993). The presence of similar sized target proteins in all tissues suggested that these serine/threonine protein kinases were ubiquitous. In binding the active GTP-forms of Cdc42 and Racl, the α-and γ-PAKs also decrease intrinsic GTP hydrolysis and block action of GTPase activating proteins (GAPs). This therefore favours signalling to PAK. However, βPAK may be released after activation allowing for amplification of the signal or initiation of other p21 actions(Manser et al 1995, Manser et al 1994).
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Manser, E., Lim, L. (1999). Roles of PAK Family Kinases. In: Jeanteur, P. (eds) Cytoskeleton and Small G Proteins. Progress in Molecular and Subcellular Biology, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58591-3_6
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