Electrical Activity of Individual Neurons: Patch-Clamp Techniques



During the last two decades the patch-clamp technique has become one of the major tools of modern electrophysiology. Originally used for measurements of single-channel currents (Neher and Sakmann, 1976; developed by Hamill et al., 1981), it has turned out to be a powerful method in studying cell excitability, functions and pharmacology of ionic channels as well as mechanisms of their regulation by different metabolic factors. Several recording configurations of the patch-clamp technique enable investigation of macroscopic currents of entire cells as well as elementary single-channel currents in microscopic membrane pieces (patches). An important advantage of the method is the possibility to make recordings under conditions where voltages and solutions at both sides of the membrane are controlled and can be manipulated during the experiment.


Internal Solution Patch Pipette Membrane Patch Liquid Junction Potential Perforated Patch 
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  1. Barry PH, Lynch JW (1991) Liquid junction potentials and small cell effects in patch-clamp analysis [published erratum appears in J Membr Biol 1992 Feb;125(3):286]. Journal of Membrane Biology 121: 101–117PubMedCrossRefGoogle Scholar
  2. Bottenstein JE, Sato G (1985) Cell cultures in the neurosciences. Plenum Press, New YorkCrossRefGoogle Scholar
  3. Corey DP, Stevens CF (1983) Science and technology of patch-recording electrodes. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 53–68CrossRefGoogle Scholar
  4. Edwards FA, Konnerth A, Sakmann B, Takahashi T (1989) A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system. Pflugers Archiv 414: 600–612PubMedCrossRefGoogle Scholar
  5. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Archiv 391: 85–100PubMedCrossRefGoogle Scholar
  6. Hilgemann DW (1995) The giant membrane patch. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 307–327Google Scholar
  7. Horn R, Marty A (1988) Muscarinic activation of ionic currents measured by a new whole-cell recording method. Journal of General Physiology 92: 145–159PubMedCrossRefGoogle Scholar
  8. Korn SJ, Marty A, Conner JA, Horn R (1991) Perforated patch recording. Methods in Neurosciences 4: 264–273Google Scholar
  9. Lindau M, Fernandez JM (1986) IgE-mediated degranulation of mast cells does not require opening of ion channels. Nature 319: 150–153PubMedCrossRefGoogle Scholar
  10. Marty A, Neher E (1995) Tight-seal whole-cell recording. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 31–52Google Scholar
  11. Neher E (1992) Correction for liquid junction potentials in patch clamp experiments. Methods in Enzymology 207: 123–131PubMedCrossRefGoogle Scholar
  12. Neher E, Sakmann B (1976) Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature 260: 799–802PubMedCrossRefGoogle Scholar
  13. Rae J, Cooper K, Gates P, Watsky M (1991) Low access resistance perforated patch recordings using amphotericin B. Journal of Neuroscience Methods 37: 15–26PubMedCrossRefGoogle Scholar
  14. Rae JL, Levis RA (1992) Glass technology for patch clamp electrodes. Methods in Enzymology 207: 66–92PubMedCrossRefGoogle Scholar
  15. Safronov BV, Vogel W (1995) Single voltage-activated Na+ and K+ channels in the somata of rat motoneurones. Journal of Physiology 487: 91–106PubMedGoogle Scholar
  16. Safronov BV, Wolff M, Vogel W (1997) Functional distribution of three types of Na+ channel on soma and processes of dorsal horn neurones of rat spinal cord. Journal of Physiology 503: 371–385PubMedCrossRefGoogle Scholar
  17. Sakmann B, Neher E (1995) Geometric parameters of pipettes and membrane patches. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 637–650CrossRefGoogle Scholar
  18. Sather W, Dieudonne S, MacDonald JF, Ascher P (1992) Activation and desensitization of N-methyl-D-aspartate receptors in nucleated outside-out patches from mouse neurones. Journal of Physiology 450: 643–672PubMedGoogle Scholar
  19. Standen NB, Stanfield PR (1992) Patch clamp methods for single channel and whole cell recording. In: Stamford JA (ed) Monitoring neuronal activity. Oxford University Press, New York, pp 59–83Google Scholar
  20. Stuart GJ, Dodt HU, Sakmann B (1993) Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflugers Archiv 423: 511–518PubMedCrossRefGoogle Scholar
  21. Trube G (1983) Enzymatic dispersion of heart and other tissues. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 69–76CrossRefGoogle Scholar
  22. Yellen G (1982) Single Ca2+-activated nonselective cation channels in neuroblastoma. Nature 296: 357–359PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1999

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