Electrical Activity of Individual Neurons: Patch-Clamp Techniques
During the last two decades the patch-clamp technique has become one of the major tools of modern electrophysiology. Originally used for measurements of single-channel currents (Neher and Sakmann, 1976; developed by Hamill et al., 1981), it has turned out to be a powerful method in studying cell excitability, functions and pharmacology of ionic channels as well as mechanisms of their regulation by different metabolic factors. Several recording configurations of the patch-clamp technique enable investigation of macroscopic currents of entire cells as well as elementary single-channel currents in microscopic membrane pieces (patches). An important advantage of the method is the possibility to make recordings under conditions where voltages and solutions at both sides of the membrane are controlled and can be manipulated during the experiment.
KeywordsInternal Solution Patch Pipette Membrane Patch Liquid Junction Potential Perforated Patch
Unable to display preview. Download preview PDF.
- Hilgemann DW (1995) The giant membrane patch. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 307–327Google Scholar
- Korn SJ, Marty A, Conner JA, Horn R (1991) Perforated patch recording. Methods in Neurosciences 4: 264–273Google Scholar
- Marty A, Neher E (1995) Tight-seal whole-cell recording. In: Sakmann B, Neher E (eds) Single-channel recording. Plenum Press, New York, pp 31–52Google Scholar
- Standen NB, Stanfield PR (1992) Patch clamp methods for single channel and whole cell recording. In: Stamford JA (ed) Monitoring neuronal activity. Oxford University Press, New York, pp 59–83Google Scholar