In-vivo Optical Imaging of Cortical Architecture and Dynamics

  • Amiram Grinvald
  • D. Shoham
  • A. Shmuel
  • D. Glaser
  • I. Vanzetta
  • E. Shtoyerman
  • H. Slovin
  • C. Wijnbergen
  • R. Hildesheim
  • A. Arieli


A number of new imaging techniques are available to scientists to visualize the functioning brain directly, revealing unprecedented details. These imaging techniques have provided a new level of understanding of the principles underlying cortical development, organization and function. In this chapter we will focus on optical imaging in the living mammalian brain, using two complementary imaging techniques. The first technique is based on intrinsic signals. The second technique is based on voltage-sensitive dyes. Currently, these two optical imaging techniques offer the best spatial and temporal resolution, but also have inherent limitations. We shall provide a few examples of new findings obtained mostly in work done in our laboratory. The focus will be upon the understanding of methodological aspects which in turn should contribute to optimal use of these imaging techniques. General reviews describing earlier work done on simpler preparations have been published elsewhere (Cohen, 1973; Tasaki and Warashina, 1976; Waggoner and Grinvald, 1977; Waggoner, 1979; Salzberg, 1983; Grinvald, 1984; Grinvald et al., 1985; De Weer and Salzberg, 1986; Cohen and Lesher, 1986; Salzberg et al., 1986; Loew, 1987; Orbach, 1987; Blasdel, 1988, 1989; Grinvald et al., 1988; Kamino, 1991; Cinelli and Kauer, 1992; Frostig, 1994).


Optical Imaging Optical Signal Local Field Potential Ocular Dominance Intrinsic Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Amiram Grinvald
  • D. Shoham
  • A. Shmuel
  • D. Glaser
  • I. Vanzetta
  • E. Shtoyerman
  • H. Slovin
  • C. Wijnbergen
  • R. Hildesheim
  • A. Arieli

There are no affiliations available

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