Abstract
Leaves of Atropa belladonna L. (Solanaceae) are mainly used as a source for the tropane alkaloids, hyoscyamine and scopolamine, which are not easily produced by chemical synthesis. Thus because of its high morphogenetic potential, the in vitro culture of A. belladonna has been actively investigated as an alternative to field cultivation and as a possible source for tropane alkaloid production (for review see Bajaj and Simola 1991) Several studies have confirmed that the product levels in unorganized A. belladonna callus and suspension cultures are generally very low and that root morphology is required for synthesis of tropane alkaloid in vitro. However, molecular genetic technology is now available to achieve transformation and regeneration of transgenic A. belladonna plants in which morphological traits and/or particular metabolic step(s) are artificially modified. The hairy root system is demonstrated to be much more genetically stable than dedifferentiated normal (callus) or transformed (crown gall) plant cells. In this chapter some important recent studies related to the genetic transformation of A. belladonna are discussed.
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Jaziri, M., Yoshimatsu, K., Shimomura, K. (1999). Genetic Transformation of Atropa belladonna . In: Bajaj, Y.P.S. (eds) Transgenic Medicinal Plants. Biotechnology in Agriculture and Forestry, vol 45. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58439-8_5
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DOI: https://doi.org/10.1007/978-3-642-58439-8_5
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