Skip to main content

Laser in Assisted Reproduction

  • Chapter
Book cover Manual on Assisted Reproduction

Abstract

Lasers were initially used for the treatment of male subfertility. The aim was to overcome fertilization failures in patients with severe oligoasthenoteratozoospermia (OAT). In these patients, the zona pellucida was found to be an obstacle which the low number of slowly moving spermatozoa present in OAT patients were unable to overcome. One approach was to create an artificial opening into the zona pellucida which should allow sperm cells to gain access to the perivitelline space and to fertilize the oocyte. Several methods for opening the zona pellucida have been proposed, based on mechanical or chemical methods. Mechanical zona opening involves the use of a sharp glass capillary mainly to cut or dissect the zona (zona cutting, Tsunoda et al. 1986; partial zona dissection, Malter and Cohen 1989). The chemical opening of the zona is achieved with acidic Tyrode’s solution (Gordon and Talansky 1987). The use of these techniques have been described in detail in the literature (Depypere et al. 1988, Cohen 1992).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Antinori S, Selman HA, Caffa B eta1 (1996a) Zona opening of human embryos using a non-contact UV laser for assisted hatching in patients with poor prognosis of pregnancy. Hum Reprod 11:2488–2492

    Article  PubMed  CAS  Google Scholar 

  • Antinori S, Panci C, Selman HA etal (1996b) Zona thinning with the use of laser: a new approach to assisted hatching in humans. Hum Reprod 11:590–594

    Article  PubMed  CAS  Google Scholar 

  • Araujo E, Tadir Y, Patrizio P etal (1994) Relative force of human epididymal sperm. Fertil Steril 62:585–590

    PubMed  Google Scholar 

  • Ashkin A, Dziedzic JM (1987) Optical trapping and manipulation of viruses and bacteria. Science 235:1517–1520

    Article  PubMed  CAS  Google Scholar 

  • Ashkin A, Dziedzic JM, Yamane T (1987) Optical trapping and manipulation of single cells using infrared laser beams. Nature 330:769–771

    Article  PubMed  CAS  Google Scholar 

  • Bider D, Livshits A, Yonish M et al (1997) Assisted hatching by zona drilling of human embryos in women of advanced age. Hum Reprod 12:317–320

    Article  PubMed  CAS  Google Scholar 

  • Boada M, Carrera M, De La Iglesia C et al (1997) Successful use of a laser for human embryo biopsy in preimplantation genetic diagnosis: report of two cases. J Assist Reprod Genet 15:301–305

    Google Scholar 

  • Burkman LJ, Coddington CC, Franken DR, Kruger TF, Rosenwaks Z, Hodgen GD (1988) The hemizona assay (HZA): development of a diagnostic test for the binding of human spermatozoa to the human hemizona pellucida to predict fertilization potential. Fertil Steril 49:688–697

    PubMed  CAS  Google Scholar 

  • Check JH, Hoover L, Nazari A, O’Shaughnessy A, Summers D (1996) The effect of assisted hatching on pregnancy rates after frozen embryo transfer. Fertil Steril 65:254–257

    PubMed  CAS  Google Scholar 

  • Clement-Sengewald A, Schütze K, Ashkin A et al (1996) Fertilization of bovine oocytes induced solely with combined laser microbeam and optical tweezers. J Assist Reprod Genet 13:259–265

    Article  PubMed  CAS  Google Scholar 

  • Cohen J (1991) Assisted hatching of human embryos. J In Vitro Fertil Embryo Transf 8:179–190

    Article  CAS  Google Scholar 

  • Cohen J (1992) Zona pellucida micromanipulation and consequences for embryonic development and implantation. In: Cohen J, Malter HE, Talansky BE, Grifo J (eds) Micromanipulation of human gametes and embryos. Raven, New York, pp 191–222

    Google Scholar 

  • Cohen J, Feldberg D (1991) Effects of the size and number of zona pellucida openings on hatching and trophoblast outgrowth in the mouse embryo. Mol Reprod Dev 30:70–78

    Article  PubMed  CAS  Google Scholar 

  • Cohen J, Alikani M, Trowbridge J, Rosenwaks Z (1992) Implantation enhancement by selec-tive assisted hatching using zona drilling of human embryos with poor prognosis. Hum Reprod 7:685–691

    PubMed  CAS  Google Scholar 

  • Cohen J, Garrisi GJ, Congedo-Ferrara TA et a1 (1997) Cryopreservation of single human spermatozoa. Hum Reprod 12:994–1001

    Article  PubMed  CAS  Google Scholar 

  • Cole RJ (1967) Cinemicrographic observation on the trophoblast and zona pellucida of the mouse blastocyst. J Embryol Exp Morphol 17:481–490

    PubMed  CAS  Google Scholar 

  • Colon JM, Sarosi P, McGovern PGet a1 (1992) Controlled micromanipulation of human sperm in three dimensions with an infrared laser optical trap: effect on sperm velocity. Fertil Steril 57:695–698

    PubMed  CAS  Google Scholar 

  • Dantas ZN, Araujo E, Tadir Y et al (1995) Effect of freezing on the relative escape force of sperm as measured by a laser optical trap. Fertil Steril 63:185–188

    PubMed  CAS  Google Scholar 

  • DeFelici M, Siracusa G (1982) Spontaneous hardening of the zona pellucida of mouse oocytes during in vitro culture. Gamete Res 6:107–113

    Article  Google Scholar 

  • Depypere HAT, McLaughlin KJ, Seamark RF et al (1988) Zona cutting and zona drilling for assisted fertilization in the mouse. J Reprod Fertil 84:205–211

    Article  PubMed  CAS  Google Scholar 

  • Downs SM, Schroeder AC, Eppig JJ (1986) Serum maintains the fertilizability of mouse oocytes matured in vitro by preventing hardening of the zona pellucida. Gamete Res 15:115–122

    Article  Google Scholar 

  • El-Danasouri I, Westphal LM, Neev Y et al (1993) Zona opening with 308 nm XeCI excimer laser improves fertilization by spermatozoa from long-term vasectomized mice. Hum Re-prod 8:464–466

    CAS  Google Scholar 

  • Enginsu ME, Schütze K, Bellanca S et al (1995) Micromanipulation of mouse gametes with laser microbeam and optical tweezers. Hum Reprod 10:1761–1764

    PubMed  CAS  Google Scholar 

  • Feichtinger W, Obruca A, Brunner M (1995) Sex chromosomal abnormalities and intracytoplasmic injection. Lancet 346:1566

    Article  PubMed  CAS  Google Scholar 

  • Gamzu R, Yogev L, Amit A et al (1994) The hemizona assay is of good prognostic value for the ability of sperm to fertilize oocytes in vitro. Fertil Steril 62:1056–1059

    PubMed  CAS  Google Scholar 

  • Germond M, Nocera D, Senn A, Rink K, Delacrétaz G, Fakan S (1995) Microdissection of mouse and human zona pellucida using a 1.48-µm diode laser beam: efficacy and safety of the procedure. Fertil Steril 25:604–611

    Google Scholar 

  • Germond M, Nocera D, Senn Aet a1 (1996) Improved fertilization and implantation rates after non-touch zona pellucida microdrilling of mouse oocytes with a 1.48-gm diode laser beam. Hum Reprod 11:1043–1048

    Article  PubMed  CAS  Google Scholar 

  • Germond M, Primi MP, Senn A et al (1998) Diode laser for assisted hatching: preliminary results of a multicentric prospective randomized study. Hum Reprod 13 Abstract Book 1:84–85

    Google Scholar 

  • Gordon JW, Talansky BE (1987) Assisted fertilization by zona drilling: a mouse model for correction of oligospermia. J Exp Zool 239:347–381

    Article  Google Scholar 

  • Grifo J (1992) Preconception and preimplantation genetic diagnosis: polar body, blastomere, and trophectoderm biopsy. In: Cohen J, Malter HE, Talansky BE, Grifo J (eds) Micromanipulation of human gametes and embryos. Raven, New York, pp 191–222

    Google Scholar 

  • Handyside A, Kontogianni E, Hardy K, Winston RML (1990) Pregnancies from biopsied human preimplantation embryos sexed with a Y-specific DNA amplification. Nature 344:768–780

    Article  PubMed  CAS  Google Scholar 

  • Hellebault S, De Sutter P, Dozortsev D et a1 (1996) Does assisted hatching improve implantation rates after in vitro fertilization or intracytoplasmic sperm injection in all patients? A prospective randomized study. J Assist Reprod Genet 13:19–22

    Article  Google Scholar 

  • Inoue M, Wolf DP (1976) Comparative solubility properties of the zonae pellucidae of unfertilized and fertilized mouse ova. Biol Reprod 11:558–565

    Article  Google Scholar 

  • Jean M, Barriere P, Mirallie S (1996) Intracytoplasmic sperm injection without polyvinylpyrrolidone: an essential precaution? Hum Reprod 11:23–32

    Article  Google Scholar 

  • Jean M, Barriere P, Mirallie S (1997) Development of a successful ICSI programme without the use of PVP. Hum Reprod 12:1115–1116

    Article  Google Scholar 

  • Kochevar IE (1989) Cytotoxicity and mutagenicity of excimer laser radiation. Lasers Surg Med 9:440–445

    Article  PubMed  CAS  Google Scholar 

  • König K, Tadir Y, Patrizio P et al (1996) Effects of ultraviolet exposure and near infrared laser tweezers on human spermatozoa. Hum Reprod 11:2162–2164

    Article  PubMed  Google Scholar 

  • Lanzendorf SE, Nehchiri F, Mayer JF et al (1998) A prospective, randomized, double-blind study for the evaluation of assisted hatching in patients with advanced maternal age. Hum Reprod 13:409–413

    Article  PubMed  CAS  Google Scholar 

  • Liu HC, Cohen J, Alikani M et a1 (1993) Assisted hatching facilitates earlier implantation. Fertil Steril 60:871–875

    PubMed  CAS  Google Scholar 

  • Magli MC, Gianaroli L, Ferraretti AP et al (1998) Rescue of implantation potential in embryos with poor prognosis by assisted hatching. Hum Reprod 13:1331–1335

    Article  PubMed  CAS  Google Scholar 

  • Malter HE, Cohen J (1989) Partial zona dissection of the human oocytes: a non-traumatic method using a micromanipulation to assist zona pellucida penetration. Fertil Stern 51:139–148

    CAS  Google Scholar 

  • McDermott A, Ray B (1996) Intracytoplasmic sperm injection without polyvinylpyrrolidone: an essential precaution? Hum Reprod 11:2332

    Article  PubMed  CAS  Google Scholar 

  • McLaren (1968) A study of blastocysts during delay and subsequent implantation in lactating mice. J Endocrinol 42:453–463

    Article  PubMed  CAS  Google Scholar 

  • Montag M, van der Ven H (1999) Laser assisted hatching in assisted reproduction. Croatian Mea J 40:398–403

    CAS  Google Scholar 

  • Montag M, van der Ven K, Delacrétaz G, Rink K, van der Ven H (1997) Efficient preimplantation genetic diagnosis using laser assisted microdissection of the zona pellucida for polar body biopsy followed by primed in situ labeling (PRINS). J Assist Reprod Genet 14:476

    Google Scholar 

  • Montag M, van der Ven K, Delacrétaz G, Rink K, van der Ven H (1998) Laser-assisted microdissection of zona pellucida facilitates polar body biopsy. Fertil Steril 69:539–542

    Article  PubMed  CAS  Google Scholar 

  • Montag M, Dieckmann U, Rink K et a1 (1999a) Laser-assisted cryopreservation of single human spermatozoa in cell-free zona pellucida. Andrologia 32:49–53

    Article  Google Scholar 

  • Montag M, Lemola R, van der Ven H (1999b) Fast and efficient production of equally sized hemizonae using a 1.48-µm diode laser system and their use in the hemizona assay. Hum Reprod 14 (Abstract book 1):144

    Article  Google Scholar 

  • Montag M, Rink K, Descloux L et al (1999c) The use of a 1.48-µm diode laser system in assisted reproduction: laser-drilling of the zona pellucida and laser-assisted immobilization of spermatozoa. Assist Reprod Rev 9:205–213

    Google Scholar 

  • Montag M, Rink K, Delacrétaz G, van der Ven H (1999d) Hardness of the zona pellucida at different stages of mouse embryonic development in vitro and in vivo as measured indirectly by a 1.48-µm diode laser. Hum Reprod 14 (Abstract book 1):168

    Google Scholar 

  • Munné S, Dailey T.Sultan KM et a1 (1995) The use of first polar bodies for preimplantation diagnosis of aneuploidy. Mol Hum Reprod 10:1014–1020

    Google Scholar 

  • Neev J, Tadir Y, Asch R et al (1992a) Laser zona dissection using short power UV lasers. Proc Soc Photo-optical Instrumentation (SPIE) 1650:62–69

    Google Scholar 

  • Neev J, Tadir Y, Ho P et a1 (1992b) Microscope delivered ultraviolet laser zona dissection: principles and practices. J Assist Reprod Genet 9:513–523

    Article  Google Scholar 

  • Neev J, Gonzalez A, Licciardi F et al (1993) Opening of the mouse zona pellucida by laser without a micromanipulator. Hum Reprod 8:939–944

    PubMed  CAS  Google Scholar 

  • Obruca A, Strohmer H, Sakkas D et al (1994) Use of lasers in assisted fertilization and hatching. Hum Reprod 9:1723–1726

    PubMed  CAS  Google Scholar 

  • Palanker D, Ohad S, Lewis A et a1 (1991) Technique for cellular microsurgery using the 193-nm excimer laser. Lasers Surg Med 11:580–586

    Article  PubMed  CAS  Google Scholar 

  • Palermo G, Joris H, Devroey P, Van Steirteghem A (1992) Pregnancies after intracytoplas-mic sperm injection of a single spermatozoon into an oocyte. Lancet 340:17–18

    Article  PubMed  CAS  Google Scholar 

  • Reubinoff BE, Shushan A (1996) Preimplantation diagnosis in older patients: to biopsy or not to biopsy. Hum Reprod 11:2071–2075

    Article  PubMed  CAS  Google Scholar 

  • Rink K, Delacrétaz G, Salathé RP et al (1994) 1.48-µm diode laser microdissection of the zona pellucida of mouse zygotes. Proc SPIE 213A:412–422

    Google Scholar 

  • Rink K, Delacrétaz G, Salathé RP et al (1996) Non-contact microdrilling of mouse zona pellucida with an objective-delivered 1.48-µm diode laser. Lasers Surg Med 18:52–62

    Article  PubMed  CAS  Google Scholar 

  • Sanchez R, Finkenzeller C, Schill WB, Miska W (1995) Comparison of two methods to obtain hemizonae pellucidae for sperm function tests. Hum Reprod 10:2945–2947

    PubMed  CAS  Google Scholar 

  • Schiewe MC, Hazeleger NL, Sclimenti C, Balmaceda JP (1995) Physiological characterization of blastocyst hatching mechanisms by use of a mouse antihatching model. Fertil Steril 63:288–294

    PubMed  CAS  Google Scholar 

  • Schoolcraft WB, Schlenker T, Gee M et al (1994) Assisted hatching in the treatment of poor prognosis in vitro fertilization candidates. Fertil Steril 62:551–554

    PubMed  CAS  Google Scholar 

  • Schoysman R, Vanderzwalmen P, Segal-Bertin G, van de Casseye M (1993) Successful fertilization by testicular spermatozoa in an in-vitro fertilization programme. Hum Reprod 8:1339–1340

    PubMed  CAS  Google Scholar 

  • Schütze K, Clement-Sengewald A, Ashkin A (1994) Zona drilling and sperm insertion with combined laser microbeam and optical tweezers. Fertil Steril 61:783–786

    PubMed  Google Scholar 

  • Stein A, Rufas O, Amit S et al (1995) Assisted hatching by partial zona dissection of human pre-embryos in patients with recurrent implantation failure after in-vitro fertilization. Fertil Steril 63:838–841

    PubMed  CAS  Google Scholar 

  • Strehler E, Baccetti B, Sterzik K et al (1998) Detrimental effects of polyvinylpyrrolidone on the ultrastructure of spermatozoa (Notulae seminologicae 13). Hum Reprod 13:120–123

    Article  PubMed  CAS  Google Scholar 

  • Strohmer H, Feichtinger W (1992) Successful clinical application of laser for micromanipulation in an in vitro fertilization program. Fertil Steril 58:212–214

    PubMed  CAS  Google Scholar 

  • Tadir Y, Wright W, Berns M (1989a) Cell micromanipulation with laser beams. In: Capitanio GL, Asch RH, De Cecco L et al (eds) G.I.F.T. from basics to clinics. Raven, New York, pp 359–368

    Google Scholar 

  • Tadir Y, Wright WH, Vafa O et al (1989b) Micromanipulation of sperm by a laser generated optical trap. Fertil Steril 52:870–873

    PubMed  CAS  Google Scholar 

  • Tadir Y, Wright W, Vafa O et al (1991) Micromanipulation of gametes using laser micro beams. Hum Reprod 6:1011–1016

    PubMed  CAS  Google Scholar 

  • Tournaye H, Devroey P, Liu J et al (1994) Microsurgical epididymal sperm aspiration and intracytoplasmic injection: a new effective approach to infertility as a result of congenital bilateral absence of the vas deferens. Fertil Steril 61:1045–1051

    PubMed  CAS  Google Scholar 

  • Tsunoda Y, Yasiu T, Nakamura K et al (1986) Effect of cutting the zona pellucida on the pro-nuclear transplantation in the mouse. J Exp Zool 240:119–125

    Article  PubMed  CAS  Google Scholar 

  • Tucker MJ, Cohen J, Massey JB et a1 (1991) Partial zona dissection of the zona pellucida of frozen thawed human embryos may enhance blastocyst hatching, implantation, and pregnancy rates. Am J Obstet Gynecol 165:341–345

    PubMed  CAS  Google Scholar 

  • Tucker MJ, Morton PC, Wright G et al (1996) Enhancement of outcome from intracytoplasmic sperm injection: does co-culture or assisted hatching improve implantation rates? Hum Reprod 11:2434–2437

    Article  PubMed  CAS  Google Scholar 

  • Verlinsky Y, Ginsberg N, Lifchez A et al (1990) Analysis of the first polar body: preconception genetic diagnosis. Hum Reprod 5:826–829

    PubMed  CAS  Google Scholar 

  • Verlinsky Y, Kuliev A (1996) Preimplantation diagnosis of common aneuploidies in infertile couples of advanced maternal age. Hum Reprod 11:2076–2077

    Article  PubMed  CAS  Google Scholar 

  • Westphal LM, ElDanasouri I, Shimizu S et al (1993) Exposure of human spermatozoa to the cumulus-oophorus results in increased relative force as measured by a 760-nm laser optical trap. Hum Reprod 8:1083–1086

    PubMed  CAS  Google Scholar 

  • Wright RW, Watson JG, Chaykin S (1978) Factors influencing the in vitro hatching of mouse blastocysts. Anim Reprod Sci 1:181–188

    Article  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Montag, M., Rink, K., Delacrétaz, G., van der Ven, H. (2000). Laser in Assisted Reproduction. In: Rabe, T., Diedrich, K., Strowitzki, T. (eds) Manual on Assisted Reproduction. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58341-4_18

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-58341-4_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-63543-4

  • Online ISBN: 978-3-642-58341-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics