Abstract
Ovarian hormone stimulation is accompanied in most assisted reproduction therapies by the monitoring of follicular growth using ultrasonographic investigations. In addition, the amount of estrogen and luteinizing hormone in peripheral blood plasma is assessed to determine the optimal time for ovulation induction. The therapeutic goal is to achieve an appropriate number of mature oocytes, for example, for in vitro fertilization or intracytoplasmic sperm injection (ICSI), because these seem to guarantee superior-quality embryos. However, well-monitored ovarian stimulation does not necessarily always guarantee mature oocytes. Since no reliable biochemical tests are available to assess follicular fluid components in the punctured fluid material, direct microscopic assessment of the morphology of the oocytes remains the most practical and promising method of evaluation. This assessment leads to a scoring of the oocyte and the resulting embryos, which bears a relation to the subsequent pregnancies. The use of a widely accepted score and photo/video documentation would be an accurate base for a comparison between different laboratories. The cumulus-oocyte complex, which has to be seen as a functional unit, contains the cumulus cells, the densely packed corona radiata cells, and the oocyte itself with the zona pellucida (Fig. 1).
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References
Balakier H, Caspar RF (1991) A morphologic study of unfertilized oocytes and abnormal embryos in human in vitro fertilization. J In Vitro Fertil Embryo Transf 8:73–79
Bavister BD (1995) Culture of preimplantation embryos: facts and artefacts Hum Reprod Update 1:91–148
Bedford JM (1998) Mammalian fertilization misread? Sperm penetration of the eutherian zona pellucida is unlikely to be a lytic event. Biol Reprod 59:1275–1287
Behboodi E, Anderson GB, Bondurant RH, Cargil SL, Kreuscher BR, Medrano JF, Murray JD (1995) Birth of large calves that developed from in-vitro-derived bovine embryos. Theriogenology 44:227–232
Behr B (1999) Bastocyst culture and transfer. Hum Reprod 14:5–6
Behr B, Pool TB, Milki AA, Moore D, Gebhardt J, Dasig D (1999) Preliminary clinical experience with human blastocyst development in vitro without co-culture. Hum Reprod 14:454–457
Beier HM (1992) Die molekulare Biologie der Befruchtungskaskade and der beginnenden Embryonalentwicklung. Ann Anat 174:491–508
Bertrand E, Van Den Bergh M, Englert Y (1995) Does zona pellucida thickness influence the fertilization rate? Hum Reprod 10:1189–1193
Bled JD, Wassarman PM (1986) Autoradiographic visualization of the mouse egg’s sperm receptor bound to sperm. J Cell Biol 102:1363–1371
Bolton VN, Wren ME, Parsons JH (1991) Pregnancies after in vitro fertilization and transfer of human blastocysts. Fertil Steril 55:830–832
Chan PJ (1987) Developmental potential of human oocytes according to zona pellucida thickness. J In Vitro Fertil Embryo Transf 4:237–241
Claman P, Arrant DR, Seibel MM, Wang TA, Oskowitz SP, Taymor ML (1987) The impact of embryo quality and quantity on implantation and the establishment of viable pregnancies. J In Vitro Fertil Embryo Transf 4:218–222
Clark DA, Lee S, Fishell S (1989) Immunosuppressive activity in human in-vitro fertilization (IVF) culture supernatants and prediction of outcome of embryo transfer: a multicenter trial. J In Vitro Fertil Embryo Transf 6:51–58
Cohen J, Gilligan A, Willadsen S (1998) Culture and quality control of embryos. Hum Re-prod 13 [Suppl 3]:137–144
Conaghan J, Hardy K, Handyside MH, Winston RML, Leese HJ (1993) Selection criteria for human embryo transfer - a comparison of pyruvate uptake and morphology. J Assist Re-prod Genet 10:21–30
Cummins JM, Breen TM, Harrison KL, Shaw JM, Wilson LM, Hennessey JF (1986) A formula for scoring human embryo growth rates in in vitro fertilization: its value in predicting pregnancy and in comparison with visual estimates of embryo quality. J In Vitro Fertil Embryo Transf 3:284–295
Desai NN (1998) The road of blastocyst transfer. Hum Reprod 13:3292–3295
De Vos A, Van Steirteghem A (2000) Tona hardening, zona drilling and assisted hatching - new achievements in assisted reproduction. Cells Tissue Organs 166:220–227
Ding J Rana N, Dmowski WP (1999) Intracytoplasmic sperm injection into zona-free human oocytes results in normal fertilization and blastocyst development. Hum Reprod 14:476–478
Dozortsev D, Rybouchkin A, De Sutter P, Qian C, Dhont M (1995) Human oocyte activation following intracytoplasmic injection: the role of sperm cell. Hum Reprod 10:403–407
Edwards RG (1980) Conception in the human female. Academic, London
Edwards RG, Hollands P (1988) New advances in human embryology: implications of the preimplantation diagnosis of genetic disease. Hum Reprod 3:549–556
Edwards RG, Beard HK (1997) Oocyte polarity and cell determination in early mammalian embryos. Mol Hum Reprod 3:863–905
Edwards RG, Beard HK (1999) Is the success of human IVF more a matter of genetics and evolution than growing blastocysts? Hum Reprod 14:1–4
Englert Y, Puissant F, Camus M, Degueldre M, Leroy F (1986) Factors leading to tripronucleate eggs during human in-vitro fertilization. Hum Reprod 1:117–119
Eppig JJ, Schultz RM, O’Brien M, Chesnel F (1994) Relationship between the developmental programs controlling nuclear and cytoplasmic maturation of mouse oocytes. Develop Biol 164:1–9
Farin PW, Farin CE (1995) Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development. Biol Reprod 52:676–682
Fischer B, Schumacher A, Hegele-Hartung C, Beier HM (1988) Potential risk of light androom temperature exposure to preimplantation embryos. Fertil Steril 50:938–944
Flaherty SP, Payne D, Swann NJ, Matthews CD (1995) Aetiology of failed and abnormal fertilization after intracytoplasmic sperm injection. Hum Reprod 10:2623–2629
Gardner DK, Lane M (1997) Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum Reprod Update 3:367–382
Gardner DK, Lane M (1998) Culture of viable human blastocysts in defined sequential serum-free media. Hum Reprod 13 [Suppl 3]:148–159
Gardner DK, Schoolcraft WB (1998) No longer neglected: the human blastocyst. Hum Re-prod 13:3289–3292
Gardner DK, Schoolcraft WB, Wagley L, Schlenker T. Stevens J, Hesla J (1998) A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization. Hum Reprod 13:3434–3440
Giorgetti C, Terriou P, Auquier P, Hans E, Spach J-L, Salzmann J, Roulier R (1995) Embryo score to predict implantation after in-vitro fertilization: based on 957 single embryo transfers. Hum Reprod 10:2427–2431
Grillo JM, Gamerre M, Lacroix O, Noizet A, Vitry G (1991) Influence of the morphological aspect of embryos obtained by in vitro fertilization on their implantation rate. J In Vitro Fertil Embryo Transf 8:317–321
Grundker C, Kirchner C (1996) Influence of uterine growth factors on blastocyst expansion and trophoblast knob formation in the rabbit. Early Pregnancy 2:264–270
Harvey MB, Kaye PL (1992) Insulin-like growth factor-1 stimulates growth of mouse preimplantation embryos in vitro. Mo1 Reprod Dev 31:195–199
Healer A, Krusche CA, Beier HM (1998) Insulin and insulin-like growth factor-1 promote rabbit blastocyst development and prevent apoptosis. Biol Reprod 59:1302–1310
Hill GA, Freeman M, Bastias MC, Rogers BJ, Herbert CM III, Osteen KG, Wentz AC (1989) The influence of oocyte maturity and embryo quality on pregnancy rate in a program for in vitro fertilization-embryo transfer. Fertil Steril 52:801–806
Holm P, Walker SK, Seamark RF (1996) Embryo viability, duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo. J Reprod Fertil 107:175–181
Hrabe de Angelis M, Grundker C, Herrmann BG, Kispert A, Kirchner C (1995) Promotion of gastrulation by maternal growth factor in cultured rabbit blastocysts. Cell Tissue Res 282:147–154
Hyttel P, Greve T, Callesen H (1988) Ultrastructure of in-vivo fertilization in superovulated cattle. J Reprod Fertil 82:1–13
Jones GM, Trounson AO, Gardner DK, Kausche A, Lolatgis N, Wood C (1998) Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod 13:169–177
Jurisicova A, Varmuza S, Casper RF (1996) Programmed cell death and human embryo fragmentation. Mol Hum Reprod 2:93–98
Kerr JFR, Winterford CM, Harmon BV (1994) Morphological criteria for identifying apoptosis. In: Celis JE (ed) Cell biology: a laboratory handbook. Academic, New York, pp 319–329
Kodama H, Fukuda J, Karube H, Matsui T, Shimizu Y, Tasdemir M, Tasdemir I, Tanaka T (1995) Prospective evaluation of simple morphological criteria for embryo selection in double embryo transfer cycles. Hum Reprod 10:2999–3003
Larson JL, Miller DJ (1997) Sperm from a variety of mammalian species express beta 1,4galactosyltransferase on their surface. Biol Reprod 57:442–453
Leese HJ, Hooper MAK, Edwards RG, Ashwood-Smith MJ (1986) Uptake of pyruvate by early human embryos determined by a non-invasive technique. Hum Reprod 1:181–182
Magnusson C, Hillensjö T, Hamberger L, Nilsson L (1986) Oxygen consumption by human oocytes and blastocysts grown in vitro. Hum Reprod 1:183–184
Menezo YJ, Ben Khalifa M (1995) Cytogenetic and cryobiology of human cocultured embryos: a 3-year experience. J Assist Reprod Genet 12:35–40
Mikkelsen AL (1999) IVM research bears fruit. FertiNet 2:http://ferti.net, special interest
Morgan K, Wiemer K, Steuerwald N, Hoffman D, Maxson W, Godke R (1995) Use of videocinematography to assess morphological qualities of conventionally cultured and cocultured embryos. Hum Reprod 10:2371–2376
Nakatsuka M, Yoshida N, Kudo T (1992) Platelet activating factor in culture media as an indicator of human embryonic development after in-vitro fertilization. Hum Reprod 7:1435–1439
O’Neill C, Gidley-Baird AA, Pike IL, Saunders DM (1987) A bio-assay for embryo-derived platelet activating factor as a means of assessing quality and pregnancy potential of human embryos. Fertil Steril 47:969–975
Palermo G, Joris H, Derde M-P, Camus M, Devroey P, Van Steirteghem AC (1993) Sperm characteristics and outcome of human assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Fertil Steril 59:826–835
Peak JG, Pilas B, Dudek EJ, Peak MJ (1991) DNA breaks caused by monochromatic 365 nm ultraviolet-A radiation or hydrogen peroxide and their repair in human epithelioid and xeroderma pigmentosum cells. Photochem Photobiol 54:197–203
Plachot M, Mandelbaum J (1990) Oocyte maturation, fertilization and embryonic growth in vitro. Br Med Bull 46:675–694
Plachot M, Crozet N (1992) Fertilization abnormalities in human in vitro fertilization. Hum Reprod 7 [Suppl]:89–94
Plachot M, de Grouchy J, Junca AM, Mandelbaum J, Turleau C, Couillin P, Cohen J, SalatBaroux J (1987) From oocyte to embryo: a model, deduced from in vitro fertilization, for natural selection against chromosome abnormalities. Ann Genet 30:22–32
Plachot M, Mandelbaum J, Junca A-M, de Grouchy J, Salat-Baroux J, Cohen J (1989) Cytogenetic analysis and developmental capacity of normal and abnormal embryos after IVF. Hum Reprod 4 [Suppl]:99–103
Puissant F, Van Rysselberge M, Barlow P, Deweze J, Leroy F (1987) Embryo scoring as a prognostic tool in IVF treatment. Hum Reprod 2:705–708
Rankin T, Familari M, Lee E, Ginsberg A, Dwyer N, Blanchette-Mackie J, Drago J, Westphal H, Dean J (1996) Mice homozygous for an insertional mutation in the Zp3 gene lack a zona pellucida and are infertile. Development 122:2903–2910
Rankin TL, Tong ZB, Castle PE, Lee E, Gore-Langton R, Nelson LM, Dean J (1998) Human Zp3 restores fertility in Zp3 null mice without affecting order-specific sperm binding. Development 125:2415–2424
Roux C, Borodkine R, Joanne C, Bresson JL, Agnani G (1995) Morphological classification of human in-vitro fertilization embryos based on the regularity of the asynchronous division process. Hum Reprod Update 1:488–496
Sakkas D (1999) The use of blastocyst culture to avoid inheritance of an abnormal paternal genome after ICS1. Hum Reprod 14:4–5
Salha O, Abusheika N, Sharma V (1998) Dynamics of human follicular growth and in-vitro oocyte maturation. Hum Reprod Update 4:816–832
Saling MP (1989) Mammalian sperm interaction with extracellular matrices of the egg. In: Milligan SR (ed) Oxford reviews of reproductive biology 11. Oxford University Press, Oxford, pp 339–388
Sathananthan AH, Ng SC, Chia CM, Lay HY, Edirisinghe WR, Raiman SS (1985) The origin and distribution of cortical granules in human oocytes with reference to Golgi nucleolar and microfilament activity. Ann NY Acad Sci 442:251–264
Scholtes MCW, Zeilmaker GH (1998) Blastocyst transfer in day-5 embryo transfer depends primarily on the number of oocytes retrieved and not on age. Fertil Steril 69:78–83
Schumacher A, Fischer B (1988) Influence of visible light and room temperature on cell proliferation in preimplantation rabbit embryos. J Reprod Fertil 84:197–204
Scott LA, Smith S (1998) The successful use of pronuclear embryo transfers the day following oocyte retrieval. Hum Reprod 13:1003–1013
Steer CV, Mills CL, Tan SL, Campbell S, Edwards RG (1992) The cumulative embryo score: a predictive embryo scoring technique to select the optimal number of embryos to transfer in an in-vitro fertilization and embryo transfer programme Hum Reprod 7:117–119
Swann K, Homa S, Carroll J (1994) An inside job: the results of injecting whole sperm into eggs support one view of signal transduction at fertilization. Hum Reprod 9:978–980
Tarin JJ, Handyside AH (1993) Embryo biopsy strategies for preimplantation diagnosis. Fertil Steril 59:943–952
Thompson JG (1997) Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants. Reprod Fertil Dev 9:341–354
Thompson JG, Gardner DK, Pugh PA, McMillan WH, Tervit HR (1995) Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos. Biol Reprod 53:1385–1391
Tsirigotis M (1998) Blastocyst stage transfer: pitfalls and benefits. Too soon to abandon current practice. Hum Reprod 13:3285–3289
Van der Ven NH, Al-Hasani S, Dietrich K, Hamerich U, Lehmann F, Krebs D (1985) Poly-spermy in in vitro fertilization of human oocytes: frequency and possible causes. Ann NY Acad Sci 442:88–95
Veeck L (ed) (1986) Atlas of the human oocyte and early conceptus. Williams & Wilkins, Baltimore, pp 1–331
Walker SK, Heard TM, Seamark RF (1992) In-vitro culture of sheep embryos without co-culture: successes and perspectives. Theriogenology 37:111–126
Wang RJ, Nixon BR (1978) Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light. In Vitro 14:715–722
Warner CM, Cao W, Exley GE, McElhinny AS, Alikani M, Cohen J, Scott RT, Brenner CA (1998) Genetic regulation of egg and embryo survival. Hum Reprod 13 [Supp13]:178–190
Wassarman PM (1987) Early events in mammalian fertilization. Annu Rev Cell Biol 3:109–142
Wolf DP, Byrd W, Dandkar P, Quigley MM (1984) Sperm concentration and the fertilization of human eggs in vitro. Biol Reprod 31:837–848
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Herrler, A., Beier, H.M. (2000). Assessment of Oocyte and Early Embryo Morphology with Regard to Embryonic Development and the Outcome of Assisted Reproduction. In: Rabe, T., Diedrich, K., Strowitzki, T. (eds) Manual on Assisted Reproduction. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-58341-4_11
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