Immunodiagnostic Aspects of Autoantibodies Against Myeloperoxidase
The past two decades have seen an explosion of data on the new group of autoantibodies known collectively as antineutrophil cytoplasmic antibodies (ANCA) which are specific for antigens in the cytoplasm (mainly primary granules) of neutrophils and monocytes. These autoantibodies were originally detected by indirect immunofluorescence (IIF) on alcohol-fixed neutrophils and two different staining patterns were described: the cytoplasmic (classic) cANCA and the perinuclear pANCA (Fig.25.1). cANCA is induced by autoantibodies directed against proteinase 3 (PR3) and pANCA is usually directed against myeloperoxidase (MPO) although other antigenic targets exist.
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