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Asymmetric Somatic Hybrids

  • Chapter
Book cover Somatic Hybridization in Crop Improvement I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 27))

Abstract

In recent years, several methods for somatic gene transfer in plants have been developed (see Bajaj 1993). If the gene of interest has been cloned, advanced transformation systems based on viral or bacterial vectors provide an efficient way of introducing it into a desired recipient genome (Potrykus 1990). Unfortunately, the genetic and biochemical basis of many economically important traits such as disease resistance, stress tolerance, yield increase, etc. are largely unknown, and these traits might be subjected to complex regulatory mechanisms which will complicate the molecular biological approaches to elucidating their genetic and biochemical basis. Therefore, although very powerful cloning methodologies are available, the isolation and characterization of these traits should be anticipated on a relatively long-term basis. For the transfer of genes which are not available as cloned DNA sequences or for transferring “blocks” of genes of agronomic interest, somatic cell hybridization via protoplast fusion represents a good alternative method (Negrutiu et al. 1989a, b; Glimelius et al. 1991).

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  1. Institute for Molecular Biology, Plant Genetics, Free University of Brussels, Paardenstraat, 65 1640, St-Genesius-Rode, Belgium

    S. Hinnisdaels, M. Jacobs & I. Negrutiu

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  1. S. Hinnisdaels
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  2. M. Jacobs
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  3. I. Negrutiu
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  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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Hinnisdaels, S., Jacobs, M., Negrutiu, I. (1994). Asymmetric Somatic Hybrids. In: Bajaj, Y.P.S. (eds) Somatic Hybridization in Crop Improvement I. Biotechnology in Agriculture and Forestry, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-57945-5_4

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